Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00770

Experiment: RCH2_defined_glucose with Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00765 and BWI76_RS00770 are separated by 150 nucleotidesBWI76_RS00770 and BWI76_RS00775 are separated by 241 nucleotidesBWI76_RS00775 and BWI76_RS00780 are separated by 94 nucleotides BWI76_RS00765: BWI76_RS00765 - DNA-binding transcriptional regulator CytR, at 155,303 to 156,331 _RS00765 BWI76_RS00770: BWI76_RS00770 - primosomal protein N', at 156,482 to 158,677 _RS00770 BWI76_RS00775: BWI76_RS00775 - 50S ribosomal protein L31, at 158,919 to 159,134 _RS00775 BWI76_RS00780: BWI76_RS00780 - met repressor, at 159,229 to 159,546 _RS00780 Position (kb) 156 157 158 159Strain fitness (log2 ratio) -1 0 1at 155.521 kb on + strand, within BWI76_RS00765at 155.521 kb on + strand, within BWI76_RS00765at 155.973 kb on - strand, within BWI76_RS00765at 156.005 kb on + strand, within BWI76_RS00765at 156.005 kb on + strand, within BWI76_RS00765at 156.005 kb on + strand, within BWI76_RS00765at 156.041 kb on + strand, within BWI76_RS00765at 156.059 kb on + strand, within BWI76_RS00765at 156.362 kb on + strandat 156.487 kb on - strandat 159.493 kb on + strand, within BWI76_RS00780at 159.623 kb on - strandat 159.654 kb on + strandat 159.654 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
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155,521 + BWI76_RS00765 0.21 -0.1
155,521 + BWI76_RS00765 0.21 -0.2
155,973 - BWI76_RS00765 0.65 -0.5
156,005 + BWI76_RS00765 0.68 +0.5
156,005 + BWI76_RS00765 0.68 -0.6
156,005 + BWI76_RS00765 0.68 -0.7
156,041 + BWI76_RS00765 0.72 -0.3
156,059 + BWI76_RS00765 0.73 +0.1
156,362 + -0.6
156,487 - +0.1
159,493 + BWI76_RS00780 0.83 +0.4
159,623 - -0.5
159,654 + -0.9
159,654 + -0.1

Or see this region's nucleotide sequence