Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT4700

Experiment: BHIS with Rifampicin 0.0000244140625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT4699 and BT4700 overlap by 4 nucleotidesBT4700 and BT4701 are separated by 19 nucleotides BT4699: BT4699 - putative hydrolase, haloacid dehalogenase-like hydrolase (NCBI ptt file), at 6,167,989 to 6,169,221 BT4699 BT4700: BT4700 - isochorismate synthase entC (NCBI ptt file), at 6,169,218 to 6,170,330 BT4700 BT4701: BT4701 - 2-oxoglutarate decarboxylase and 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (NCBI ptt file), at 6,170,350 to 6,172,017 BT4701 Position (kb) 6169 6170 6171Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 6168.336 kb on + strand, within BT4699at 6168.641 kb on + strand, within BT4699at 6168.736 kb on + strand, within BT4699at 6168.736 kb on + strand, within BT4699at 6168.882 kb on + strand, within BT4699at 6168.886 kb on + strand, within BT4699at 6168.969 kb on + strand, within BT4699at 6169.247 kb on + strandat 6169.308 kb on + strandat 6169.308 kb on + strandat 6169.412 kb on + strand, within BT4700at 6169.697 kb on + strand, within BT4700at 6169.701 kb on + strand, within BT4700at 6169.881 kb on + strand, within BT4700at 6170.112 kb on + strand, within BT4700

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Rifampicin 0.0000244140625 mM
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6,168,336 + BT4699 0.28 -1.6
6,168,641 + BT4699 0.53 -1.2
6,168,736 + BT4699 0.61 -2.2
6,168,736 + BT4699 0.61 +0.4
6,168,882 + BT4699 0.72 +0.6
6,168,886 + BT4699 0.73 -0.6
6,168,969 + BT4699 0.79 +0.4
6,169,247 + -1.2
6,169,308 + -0.6
6,169,308 + -1.1
6,169,412 + BT4700 0.17 -0.4
6,169,697 + BT4700 0.43 -2.0
6,169,701 + BT4700 0.43 -2.0
6,169,881 + BT4700 0.60 -3.9
6,170,112 + BT4700 0.80 -2.2

Or see this region's nucleotide sequence