Experiment: library passed through aphids in food, 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2214 and Psyr_2215 overlap by 4 nucleotides Psyr_2215 and Psyr_2216 are separated by 64 nucleotides Psyr_2216 and Psyr_2217 are separated by 87 nucleotides Psyr_2217 and Psyr_2218 are separated by 123 nucleotides
Psyr_2214: Psyr_2214 - PAS, at 2,573,305 to 2,575,437
_2214
Psyr_2215: Psyr_2215 - CheW-like protein, at 2,575,434 to 2,576,912
_2215
Psyr_2216: Psyr_2216 - conserved hypothetical protein, at 2,576,977 to 2,577,102
_2216
Psyr_2217: Psyr_2217 - conserved hypothetical protein, at 2,577,190 to 2,577,588
_2217
Psyr_2218: Psyr_2218 - Beta-lactamase-like protein, at 2,577,712 to 2,578,587
_2218
Position (kb)
2575
2576
2577 Strain fitness (log2 ratio)
-1
0
1 at 2574.597 kb on + strand, within Psyr_2214 at 2574.616 kb on + strand, within Psyr_2214 at 2574.616 kb on + strand, within Psyr_2214 at 2574.616 kb on + strand, within Psyr_2214 at 2574.616 kb on + strand, within Psyr_2214 at 2574.616 kb on + strand, within Psyr_2214 at 2574.617 kb on - strand, within Psyr_2214 at 2574.617 kb on - strand, within Psyr_2214 at 2574.617 kb on - strand, within Psyr_2214 at 2574.617 kb on - strand, within Psyr_2214 at 2574.674 kb on - strand, within Psyr_2214 at 2574.703 kb on + strand, within Psyr_2214 at 2574.703 kb on + strand, within Psyr_2214 at 2574.734 kb on - strand, within Psyr_2214 at 2574.734 kb on - strand, within Psyr_2214 at 2574.792 kb on + strand, within Psyr_2214 at 2574.793 kb on - strand, within Psyr_2214 at 2574.883 kb on + strand, within Psyr_2214 at 2574.883 kb on + strand, within Psyr_2214 at 2575.312 kb on - strand at 2575.375 kb on - strand at 2575.393 kb on + strand at 2575.415 kb on + strand at 2575.415 kb on + strand at 2575.480 kb on - strand at 2575.577 kb on + strand at 2575.577 kb on + strand at 2575.677 kb on + strand, within Psyr_2215 at 2575.677 kb on + strand, within Psyr_2215 at 2575.677 kb on + strand, within Psyr_2215 at 2575.689 kb on + strand, within Psyr_2215 at 2575.827 kb on + strand, within Psyr_2215 at 2575.828 kb on - strand, within Psyr_2215 at 2575.828 kb on - strand, within Psyr_2215 at 2575.828 kb on - strand, within Psyr_2215 at 2575.828 kb on - strand, within Psyr_2215 at 2575.828 kb on - strand, within Psyr_2215 at 2575.867 kb on + strand, within Psyr_2215 at 2575.867 kb on + strand, within Psyr_2215 at 2575.868 kb on - strand, within Psyr_2215 at 2575.868 kb on - strand, within Psyr_2215 at 2575.942 kb on - strand, within Psyr_2215 at 2575.942 kb on - strand, within Psyr_2215 at 2575.942 kb on - strand, within Psyr_2215 at 2576.364 kb on + strand, within Psyr_2215 at 2576.376 kb on + strand, within Psyr_2215 at 2576.945 kb on + strand at 2576.946 kb on - strand at 2577.625 kb on + strand at 2577.625 kb on + strand at 2577.625 kb on + strand at 2577.625 kb on + strand at 2577.626 kb on - strand at 2577.626 kb on - strand at 2577.637 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 24 hours remove 2,574,597 + Psyr_2214 0.61 -0.2 2,574,616 + Psyr_2214 0.61 +0.1 2,574,616 + Psyr_2214 0.61 -1.0 2,574,616 + Psyr_2214 0.61 +0.5 2,574,616 + Psyr_2214 0.61 -0.4 2,574,616 + Psyr_2214 0.61 +0.6 2,574,617 - Psyr_2214 0.62 +0.7 2,574,617 - Psyr_2214 0.62 -0.8 2,574,617 - Psyr_2214 0.62 -0.4 2,574,617 - Psyr_2214 0.62 -0.9 2,574,674 - Psyr_2214 0.64 +1.1 2,574,703 + Psyr_2214 0.66 -0.2 2,574,703 + Psyr_2214 0.66 +0.2 2,574,734 - Psyr_2214 0.67 +0.8 2,574,734 - Psyr_2214 0.67 +0.3 2,574,792 + Psyr_2214 0.70 -0.5 2,574,793 - Psyr_2214 0.70 +0.4 2,574,883 + Psyr_2214 0.74 -0.1 2,574,883 + Psyr_2214 0.74 +0.3 2,575,312 - +0.1 2,575,375 - +0.7 2,575,393 + +0.7 2,575,415 + -0.0 2,575,415 + +0.4 2,575,480 - -0.1 2,575,577 + -0.8 2,575,577 + +0.6 2,575,677 + Psyr_2215 0.16 -0.5 2,575,677 + Psyr_2215 0.16 -1.1 2,575,677 + Psyr_2215 0.16 +0.4 2,575,689 + Psyr_2215 0.17 -0.5 2,575,827 + Psyr_2215 0.27 +0.3 2,575,828 - Psyr_2215 0.27 +0.1 2,575,828 - Psyr_2215 0.27 +0.3 2,575,828 - Psyr_2215 0.27 -0.7 2,575,828 - Psyr_2215 0.27 -0.3 2,575,828 - Psyr_2215 0.27 -0.5 2,575,867 + Psyr_2215 0.29 -0.3 2,575,867 + Psyr_2215 0.29 -0.7 2,575,868 - Psyr_2215 0.29 +0.6 2,575,868 - Psyr_2215 0.29 +0.9 2,575,942 - Psyr_2215 0.34 +1.0 2,575,942 - Psyr_2215 0.34 +0.6 2,575,942 - Psyr_2215 0.34 +0.6 2,576,364 + Psyr_2215 0.63 -0.9 2,576,376 + Psyr_2215 0.64 +0.6 2,576,945 + +0.7 2,576,946 - +1.0 2,577,625 + -0.1 2,577,625 + -1.1 2,577,625 + -0.5 2,577,625 + +0.1 2,577,626 - -1.4 2,577,626 - +0.1 2,577,637 + +0.4
Or see this region's nucleotide sequence