Experiment: library passed through aphids in food, 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0221 and Psyr_0222 are separated by 96 nucleotides Psyr_0222 and Psyr_0223 are separated by 133 nucleotides Psyr_0223 and Psyr_0224 are separated by 364 nucleotides Psyr_0224 and Psyr_0225 are separated by 11 nucleotides Psyr_0225 and Psyr_0226 are separated by 81 nucleotides
Psyr_0221: Psyr_0221 - Phosphopantothenate-cysteine ligase / Phosphopantothenoylcysteine decarboxylase, at 235,108 to 236,355
_0221
Psyr_0222: Psyr_0222 - DNA replication and repair protein RadC, at 236,452 to 237,126
_0222
Psyr_0223: Psyr_0223 - extracellular solute-binding protein, family 5, at 237,260 to 238,846
_0223
Psyr_0224: Psyr_0224 - LSU ribosomal protein L28P, at 239,211 to 239,444
_0224
Psyr_0225: Psyr_0225 - LSU ribosomal protein L33P, at 239,456 to 239,611
_0225
Psyr_0226: Psyr_0226 - Protein of unknown function DUF861, at 239,693 to 240,061
_0226
Position (kb) 237
238
239 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 236.403 kb on - strand at 236.451 kb on + strand at 236.451 kb on + strand at 236.451 kb on + strand at 236.452 kb on - strand at 236.452 kb on - strand at 236.452 kb on - strand at 236.452 kb on - strand at 236.457 kb on + strand at 236.582 kb on + strand, within Psyr_0222 at 236.583 kb on - strand, within Psyr_0222 at 236.583 kb on - strand, within Psyr_0222 at 236.785 kb on + strand, within Psyr_0222 at 236.785 kb on + strand, within Psyr_0222 at 236.785 kb on + strand, within Psyr_0222 at 236.785 kb on + strand, within Psyr_0222 at 236.785 kb on + strand, within Psyr_0222 at 236.786 kb on - strand, within Psyr_0222 at 236.786 kb on - strand, within Psyr_0222 at 236.786 kb on - strand, within Psyr_0222 at 236.786 kb on - strand, within Psyr_0222 at 236.873 kb on + strand, within Psyr_0222 at 236.874 kb on - strand, within Psyr_0222 at 236.874 kb on - strand, within Psyr_0222 at 236.874 kb on - strand, within Psyr_0222 at 236.874 kb on - strand, within Psyr_0222 at 236.874 kb on - strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.906 kb on + strand, within Psyr_0222 at 236.906 kb on + strand, within Psyr_0222 at 236.906 kb on + strand, within Psyr_0222 at 236.906 kb on + strand, within Psyr_0222 at 236.906 kb on + strand, within Psyr_0222 at 236.906 kb on + strand, within Psyr_0222 at 236.906 kb on + strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 237.124 kb on + strand at 237.124 kb on + strand at 237.124 kb on + strand at 237.124 kb on + strand at 237.125 kb on - strand at 237.125 kb on - strand at 237.143 kb on + strand at 237.261 kb on + strand at 237.261 kb on + strand at 237.261 kb on + strand at 237.261 kb on + strand at 237.261 kb on + strand at 237.261 kb on + strand at 237.261 kb on + strand at 237.261 kb on + strand at 237.261 kb on + strand at 237.261 kb on + strand at 237.262 kb on - strand at 237.262 kb on - strand at 237.262 kb on - strand at 237.262 kb on - strand at 237.262 kb on - strand at 237.280 kb on - strand at 237.408 kb on + strand at 237.408 kb on + strand at 237.408 kb on + strand at 237.409 kb on - strand at 237.446 kb on - strand, within Psyr_0223 at 237.483 kb on + strand, within Psyr_0223 at 237.742 kb on + strand, within Psyr_0223 at 237.743 kb on - strand, within Psyr_0223 at 237.743 kb on - strand, within Psyr_0223 at 237.743 kb on - strand, within Psyr_0223 at 237.743 kb on - strand, within Psyr_0223 at 237.743 kb on - strand, within Psyr_0223 at 237.834 kb on + strand, within Psyr_0223 at 237.903 kb on + strand, within Psyr_0223 at 237.903 kb on + strand, within Psyr_0223 at 237.903 kb on + strand, within Psyr_0223 at 237.903 kb on + strand, within Psyr_0223 at 237.903 kb on + strand, within Psyr_0223 at 237.904 kb on - strand, within Psyr_0223 at 237.904 kb on - strand, within Psyr_0223 at 238.137 kb on + strand, within Psyr_0223 at 238.173 kb on + strand, within Psyr_0223 at 238.173 kb on + strand, within Psyr_0223 at 238.173 kb on + strand, within Psyr_0223 at 238.174 kb on - strand, within Psyr_0223 at 238.174 kb on - strand, within Psyr_0223 at 238.191 kb on + strand, within Psyr_0223 at 238.191 kb on + strand, within Psyr_0223 at 238.191 kb on + strand, within Psyr_0223 at 238.192 kb on - strand, within Psyr_0223 at 238.297 kb on + strand, within Psyr_0223 at 238.297 kb on + strand, within Psyr_0223 at 238.298 kb on - strand, within Psyr_0223 at 238.298 kb on - strand, within Psyr_0223 at 238.298 kb on - strand, within Psyr_0223 at 238.298 kb on - strand, within Psyr_0223 at 238.298 kb on - strand, within Psyr_0223 at 238.299 kb on + strand, within Psyr_0223 at 238.299 kb on + strand, within Psyr_0223 at 238.299 kb on + strand, within Psyr_0223 at 238.300 kb on - strand, within Psyr_0223 at 238.311 kb on + strand, within Psyr_0223 at 238.311 kb on + strand, within Psyr_0223 at 238.312 kb on - strand, within Psyr_0223 at 238.312 kb on - strand, within Psyr_0223 at 238.312 kb on - strand, within Psyr_0223 at 238.312 kb on - strand, within Psyr_0223 at 238.552 kb on - strand, within Psyr_0223 at 238.597 kb on - strand, within Psyr_0223 at 238.597 kb on - strand, within Psyr_0223 at 238.627 kb on + strand, within Psyr_0223 at 238.628 kb on - strand, within Psyr_0223 at 238.628 kb on - strand, within Psyr_0223 at 238.628 kb on - strand, within Psyr_0223 at 238.673 kb on + strand, within Psyr_0223 at 238.674 kb on - strand, within Psyr_0223 at 238.674 kb on - strand, within Psyr_0223 at 238.674 kb on - strand, within Psyr_0223 at 238.728 kb on + strand at 238.728 kb on + strand at 238.728 kb on + strand at 238.728 kb on + strand at 238.728 kb on + strand at 238.728 kb on + strand at 238.729 kb on - strand at 238.729 kb on - strand at 238.729 kb on - strand at 238.729 kb on - strand at 238.729 kb on - strand at 238.729 kb on - strand at 238.729 kb on - strand at 238.733 kb on + strand at 238.733 kb on + strand at 238.733 kb on + strand at 238.733 kb on + strand at 238.733 kb on + strand at 238.733 kb on + strand at 238.733 kb on + strand at 238.733 kb on + strand at 238.733 kb on + strand at 238.733 kb on + strand at 238.734 kb on - strand at 238.734 kb on - strand at 238.734 kb on - strand at 238.734 kb on - strand at 238.734 kb on - strand at 238.821 kb on - strand at 238.838 kb on + strand at 238.839 kb on - strand at 238.839 kb on - strand at 238.839 kb on - strand at 238.839 kb on - strand at 238.853 kb on - strand at 238.853 kb on - strand at 238.853 kb on - strand at 238.909 kb on + strand at 238.910 kb on - strand at 238.910 kb on - strand at 238.918 kb on + strand at 238.918 kb on + strand at 238.918 kb on + strand at 238.918 kb on + strand at 238.918 kb on + strand at 238.918 kb on + strand at 238.918 kb on + strand at 238.918 kb on + strand at 238.919 kb on - strand at 239.690 kb on + strand at 239.691 kb on - strand at 239.694 kb on + strand at 239.694 kb on + strand at 239.694 kb on + strand at 239.694 kb on + strand at 239.694 kb on + strand at 239.694 kb on + strand at 239.695 kb on - strand at 239.695 kb on - strand at 239.695 kb on - strand at 239.695 kb on - strand at 239.695 kb on - strand at 239.695 kb on - strand at 239.698 kb on + strand at 239.698 kb on + strand at 239.699 kb on - strand at 239.699 kb on - strand at 239.724 kb on + strand at 239.724 kb on + strand at 239.724 kb on + strand at 239.725 kb on - strand at 239.725 kb on - strand at 239.725 kb on - strand at 239.725 kb on - strand at 239.725 kb on - strand at 239.725 kb on - strand at 239.758 kb on + strand, within Psyr_0226 at 239.758 kb on + strand, within Psyr_0226 at 239.759 kb on - strand, within Psyr_0226 at 239.759 kb on - strand, within Psyr_0226 at 239.759 kb on - strand, within Psyr_0226
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 24 hours remove 236,403 - +0.1 236,451 + -0.7 236,451 + +0.2 236,451 + +0.1 236,452 - -0.1 236,452 - -0.8 236,452 - +0.2 236,452 - +0.4 236,457 + -0.5 236,582 + Psyr_0222 0.19 +0.4 236,583 - Psyr_0222 0.19 +0.1 236,583 - Psyr_0222 0.19 -0.0 236,785 + Psyr_0222 0.49 -2.3 236,785 + Psyr_0222 0.49 +1.0 236,785 + Psyr_0222 0.49 +0.4 236,785 + Psyr_0222 0.49 +0.3 236,785 + Psyr_0222 0.49 +0.6 236,786 - Psyr_0222 0.49 +0.5 236,786 - Psyr_0222 0.49 -0.8 236,786 - Psyr_0222 0.49 +0.6 236,786 - Psyr_0222 0.49 +0.8 236,873 + Psyr_0222 0.62 +0.6 236,874 - Psyr_0222 0.63 +0.0 236,874 - Psyr_0222 0.63 +0.4 236,874 - Psyr_0222 0.63 -0.8 236,874 - Psyr_0222 0.63 -0.2 236,874 - Psyr_0222 0.63 +0.0 236,902 + Psyr_0222 0.67 -0.1 236,902 + Psyr_0222 0.67 +0.4 236,902 + Psyr_0222 0.67 -2.9 236,902 + Psyr_0222 0.67 +0.6 236,902 + Psyr_0222 0.67 +0.0 236,902 + Psyr_0222 0.67 -0.9 236,902 + Psyr_0222 0.67 -0.3 236,902 + Psyr_0222 0.67 +0.6 236,902 + Psyr_0222 0.67 -0.9 236,902 + Psyr_0222 0.67 -0.3 236,902 + Psyr_0222 0.67 -0.3 236,902 + Psyr_0222 0.67 +0.0 236,903 - Psyr_0222 0.67 +0.6 236,903 - Psyr_0222 0.67 +0.2 236,903 - Psyr_0222 0.67 -1.3 236,903 - Psyr_0222 0.67 -0.3 236,903 - Psyr_0222 0.67 -2.2 236,903 - Psyr_0222 0.67 +0.3 236,903 - Psyr_0222 0.67 -0.1 236,903 - Psyr_0222 0.67 -0.2 236,903 - Psyr_0222 0.67 -0.5 236,903 - Psyr_0222 0.67 +0.5 236,903 - Psyr_0222 0.67 +0.1 236,903 - Psyr_0222 0.67 -1.8 236,906 + Psyr_0222 0.67 +0.4 236,906 + Psyr_0222 0.67 -0.1 236,906 + Psyr_0222 0.67 +0.4 236,906 + Psyr_0222 0.67 -0.1 236,906 + Psyr_0222 0.67 +0.2 236,906 + Psyr_0222 0.67 -0.5 236,906 + Psyr_0222 0.67 +0.2 236,907 - Psyr_0222 0.67 -0.0 236,907 - Psyr_0222 0.67 -0.3 236,907 - Psyr_0222 0.67 -1.1 236,907 - Psyr_0222 0.67 -0.2 236,907 - Psyr_0222 0.67 +0.4 236,907 - Psyr_0222 0.67 +0.7 236,907 - Psyr_0222 0.67 -1.1 236,907 - Psyr_0222 0.67 +0.2 236,907 - Psyr_0222 0.67 -0.2 236,907 - Psyr_0222 0.67 +0.3 237,124 + +0.3 237,124 + -0.2 237,124 + -0.8 237,124 + +0.5 237,125 - -0.2 237,125 - -1.0 237,143 + -0.3 237,261 + -1.3 237,261 + -0.5 237,261 + -1.1 237,261 + -0.3 237,261 + -0.6 237,261 + -0.4 237,261 + -0.6 237,261 + +0.3 237,261 + -1.5 237,261 + +0.6 237,262 - -0.3 237,262 - +0.7 237,262 - +0.8 237,262 - +0.3 237,262 - +0.1 237,280 - +0.7 237,408 + -0.4 237,408 + -1.4 237,408 + -0.9 237,409 - +1.1 237,446 - Psyr_0223 0.12 -0.5 237,483 + Psyr_0223 0.14 +0.4 237,742 + Psyr_0223 0.30 -0.7 237,743 - Psyr_0223 0.30 -0.1 237,743 - Psyr_0223 0.30 -0.5 237,743 - Psyr_0223 0.30 +0.2 237,743 - Psyr_0223 0.30 -0.7 237,743 - Psyr_0223 0.30 -0.8 237,834 + Psyr_0223 0.36 +0.3 237,903 + Psyr_0223 0.41 +0.3 237,903 + Psyr_0223 0.41 -0.2 237,903 + Psyr_0223 0.41 +0.3 237,903 + Psyr_0223 0.41 -0.7 237,903 + Psyr_0223 0.41 -0.4 237,904 - Psyr_0223 0.41 -0.7 237,904 - Psyr_0223 0.41 -1.3 238,137 + Psyr_0223 0.55 +0.1 238,173 + Psyr_0223 0.58 -0.3 238,173 + Psyr_0223 0.58 +0.2 238,173 + Psyr_0223 0.58 +0.6 238,174 - Psyr_0223 0.58 -0.8 238,174 - Psyr_0223 0.58 +0.3 238,191 + Psyr_0223 0.59 -1.7 238,191 + Psyr_0223 0.59 -1.7 238,191 + Psyr_0223 0.59 -0.3 238,192 - Psyr_0223 0.59 -0.6 238,297 + Psyr_0223 0.65 -0.3 238,297 + Psyr_0223 0.65 +0.0 238,298 - Psyr_0223 0.65 +0.9 238,298 - Psyr_0223 0.65 +0.2 238,298 - Psyr_0223 0.65 -0.2 238,298 - Psyr_0223 0.65 -0.2 238,298 - Psyr_0223 0.65 +0.3 238,299 + Psyr_0223 0.65 -0.2 238,299 + Psyr_0223 0.65 -1.3 238,299 + Psyr_0223 0.65 +0.4 238,300 - Psyr_0223 0.66 -0.3 238,311 + Psyr_0223 0.66 -0.1 238,311 + Psyr_0223 0.66 +0.6 238,312 - Psyr_0223 0.66 -0.8 238,312 - Psyr_0223 0.66 +0.3 238,312 - Psyr_0223 0.66 +0.2 238,312 - Psyr_0223 0.66 -0.6 238,552 - Psyr_0223 0.81 -1.2 238,597 - Psyr_0223 0.84 -0.3 238,597 - Psyr_0223 0.84 -1.3 238,627 + Psyr_0223 0.86 -0.5 238,628 - Psyr_0223 0.86 +0.3 238,628 - Psyr_0223 0.86 -1.2 238,628 - Psyr_0223 0.86 +0.6 238,673 + Psyr_0223 0.89 +0.6 238,674 - Psyr_0223 0.89 -0.2 238,674 - Psyr_0223 0.89 -0.0 238,674 - Psyr_0223 0.89 -0.2 238,728 + +0.8 238,728 + +1.2 238,728 + +0.2 238,728 + +0.1 238,728 + +0.0 238,728 + -0.9 238,729 - +0.1 238,729 - -0.3 238,729 - +0.2 238,729 - -0.2 238,729 - +0.9 238,729 - -0.0 238,729 - +0.0 238,733 + -0.3 238,733 + -1.1 238,733 + +0.9 238,733 + -0.2 238,733 + -0.2 238,733 + +0.2 238,733 + +0.1 238,733 + +0.7 238,733 + +0.9 238,733 + -0.2 238,734 - -0.3 238,734 - -1.1 238,734 - +0.3 238,734 - +0.1 238,734 - +0.0 238,821 - -2.4 238,838 + +0.7 238,839 - +0.1 238,839 - -1.2 238,839 - -0.8 238,839 - -0.1 238,853 - -1.0 238,853 - -1.3 238,853 - -1.3 238,909 + -0.7 238,910 - -1.0 238,910 - +0.4 238,918 + -0.2 238,918 + -1.5 238,918 + -0.6 238,918 + -0.4 238,918 + -0.5 238,918 + -0.0 238,918 + +0.2 238,918 + +0.3 238,919 - +1.4 239,690 + -2.0 239,691 - -0.4 239,694 + -0.4 239,694 + -0.3 239,694 + -0.7 239,694 + -1.6 239,694 + +0.4 239,694 + +0.0 239,695 - +0.0 239,695 - -1.1 239,695 - +0.5 239,695 - -1.7 239,695 - -0.8 239,695 - -0.1 239,698 + +0.0 239,698 + -0.1 239,699 - +0.8 239,699 - +0.1 239,724 + +0.2 239,724 + +0.1 239,724 + +1.1 239,725 - -0.3 239,725 - +0.6 239,725 - -0.8 239,725 - -0.5 239,725 - +0.8 239,725 - +0.4 239,758 + Psyr_0226 0.18 -1.0 239,758 + Psyr_0226 0.18 +0.2 239,759 - Psyr_0226 0.18 +0.1 239,759 - Psyr_0226 0.18 -0.1 239,759 - Psyr_0226 0.18 +1.2
Or see this region's nucleotide sequence