Experiment: 4-Hydroxybenzoic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5668 and Pf6N2E2_5669 are separated by 130 nucleotides Pf6N2E2_5669 and Pf6N2E2_5670 are separated by 69 nucleotides Pf6N2E2_5670 and Pf6N2E2_5671 are separated by 12 nucleotides
Pf6N2E2_5668 - Succinylglutamic semialdehyde dehydrogenase (EC 1.2.1.71), at 6,480,751 to 6,482,217
_5668
Pf6N2E2_5669 - Succinylarginine dihydrolase (EC 3.5.3.23), at 6,482,348 to 6,483,694
_5669
Pf6N2E2_5670 - Putative GTPases (G3E family), at 6,483,764 to 6,484,051
_5670
Pf6N2E2_5671 - Succinylglutamate desuccinylase (EC 3.5.1.96), at 6,484,064 to 6,485,071
_5671
Position (kb)
6482
6483
6484 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6481.540 kb on + strand, within Pf6N2E2_5668 at 6481.540 kb on + strand, within Pf6N2E2_5668 at 6481.549 kb on - strand, within Pf6N2E2_5668 at 6481.910 kb on - strand, within Pf6N2E2_5668 at 6481.947 kb on + strand, within Pf6N2E2_5668 at 6481.948 kb on - strand, within Pf6N2E2_5668 at 6481.948 kb on - strand, within Pf6N2E2_5668 at 6481.954 kb on + strand, within Pf6N2E2_5668 at 6481.955 kb on - strand, within Pf6N2E2_5668 at 6482.119 kb on + strand at 6482.123 kb on - strand at 6482.123 kb on - strand at 6482.134 kb on + strand at 6482.305 kb on + strand at 6482.306 kb on - strand at 6482.316 kb on + strand at 6482.317 kb on - strand at 6482.317 kb on - strand at 6482.318 kb on + strand at 6482.358 kb on - strand at 6482.380 kb on - strand at 6482.380 kb on - strand at 6482.395 kb on + strand at 6482.395 kb on + strand at 6482.396 kb on - strand at 6482.396 kb on - strand at 6482.399 kb on + strand at 6482.399 kb on + strand at 6482.400 kb on - strand at 6482.400 kb on - strand at 6482.400 kb on - strand at 6482.400 kb on - strand at 6482.753 kb on + strand, within Pf6N2E2_5669 at 6482.754 kb on - strand, within Pf6N2E2_5669 at 6482.754 kb on - strand, within Pf6N2E2_5669 at 6482.754 kb on - strand, within Pf6N2E2_5669 at 6482.754 kb on - strand, within Pf6N2E2_5669 at 6482.754 kb on - strand, within Pf6N2E2_5669 at 6482.754 kb on - strand, within Pf6N2E2_5669 at 6482.978 kb on + strand, within Pf6N2E2_5669 at 6482.979 kb on - strand, within Pf6N2E2_5669 at 6483.053 kb on + strand, within Pf6N2E2_5669 at 6483.054 kb on - strand, within Pf6N2E2_5669 at 6483.137 kb on + strand, within Pf6N2E2_5669 at 6483.138 kb on - strand, within Pf6N2E2_5669 at 6483.138 kb on - strand, within Pf6N2E2_5669 at 6483.138 kb on - strand, within Pf6N2E2_5669 at 6483.138 kb on - strand, within Pf6N2E2_5669 at 6483.283 kb on + strand, within Pf6N2E2_5669 at 6483.365 kb on + strand, within Pf6N2E2_5669 at 6483.366 kb on - strand, within Pf6N2E2_5669 at 6483.366 kb on - strand, within Pf6N2E2_5669 at 6483.536 kb on + strand, within Pf6N2E2_5669 at 6483.536 kb on + strand, within Pf6N2E2_5669 at 6483.659 kb on - strand at 6483.713 kb on + strand at 6483.713 kb on + strand at 6483.713 kb on + strand at 6483.757 kb on + strand at 6483.757 kb on + strand at 6483.757 kb on + strand at 6483.758 kb on - strand at 6483.758 kb on - strand at 6483.772 kb on + strand at 6483.773 kb on - strand at 6483.930 kb on - strand, within Pf6N2E2_5670 at 6484.050 kb on - strand at 6484.505 kb on + strand, within Pf6N2E2_5671 at 6484.506 kb on - strand, within Pf6N2E2_5671 at 6484.668 kb on - strand, within Pf6N2E2_5671 at 6484.673 kb on + strand, within Pf6N2E2_5671 at 6484.674 kb on - strand, within Pf6N2E2_5671 at 6484.674 kb on - strand, within Pf6N2E2_5671
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Hydroxybenzoic Acid (C) remove 6,481,540 + Pf6N2E2_5668 0.54 +1.0 6,481,540 + Pf6N2E2_5668 0.54 -1.1 6,481,549 - Pf6N2E2_5668 0.54 -2.1 6,481,910 - Pf6N2E2_5668 0.79 -2.1 6,481,947 + Pf6N2E2_5668 0.82 -0.1 6,481,948 - Pf6N2E2_5668 0.82 +0.1 6,481,948 - Pf6N2E2_5668 0.82 -0.5 6,481,954 + Pf6N2E2_5668 0.82 +0.2 6,481,955 - Pf6N2E2_5668 0.82 -0.1 6,482,119 + -0.2 6,482,123 - +1.0 6,482,123 - +0.5 6,482,134 + +0.8 6,482,305 + +1.0 6,482,306 - -0.1 6,482,316 + +0.6 6,482,317 - -0.5 6,482,317 - -1.5 6,482,318 + +0.9 6,482,358 - -0.5 6,482,380 - -0.0 6,482,380 - -2.3 6,482,395 + -0.3 6,482,395 + +0.8 6,482,396 - -0.5 6,482,396 - +1.4 6,482,399 + -0.8 6,482,399 + -0.5 6,482,400 - +0.0 6,482,400 - -1.0 6,482,400 - -2.1 6,482,400 - -0.7 6,482,753 + Pf6N2E2_5669 0.30 -2.0 6,482,754 - Pf6N2E2_5669 0.30 +1.0 6,482,754 - Pf6N2E2_5669 0.30 -0.1 6,482,754 - Pf6N2E2_5669 0.30 -1.9 6,482,754 - Pf6N2E2_5669 0.30 -0.2 6,482,754 - Pf6N2E2_5669 0.30 -0.3 6,482,754 - Pf6N2E2_5669 0.30 -0.4 6,482,978 + Pf6N2E2_5669 0.47 -0.7 6,482,979 - Pf6N2E2_5669 0.47 -1.0 6,483,053 + Pf6N2E2_5669 0.52 -0.8 6,483,054 - Pf6N2E2_5669 0.52 -1.3 6,483,137 + Pf6N2E2_5669 0.59 -0.8 6,483,138 - Pf6N2E2_5669 0.59 +0.5 6,483,138 - Pf6N2E2_5669 0.59 -0.7 6,483,138 - Pf6N2E2_5669 0.59 -1.0 6,483,138 - Pf6N2E2_5669 0.59 -3.0 6,483,283 + Pf6N2E2_5669 0.69 -2.1 6,483,365 + Pf6N2E2_5669 0.76 -2.5 6,483,366 - Pf6N2E2_5669 0.76 -0.5 6,483,366 - Pf6N2E2_5669 0.76 +2.0 6,483,536 + Pf6N2E2_5669 0.88 -1.5 6,483,536 + Pf6N2E2_5669 0.88 -0.5 6,483,659 - +1.2 6,483,713 + -0.1 6,483,713 + -0.7 6,483,713 + +0.2 6,483,757 + -0.3 6,483,757 + -1.3 6,483,757 + +1.2 6,483,758 - -0.8 6,483,758 - +1.0 6,483,772 + -0.6 6,483,773 - +2.0 6,483,930 - Pf6N2E2_5670 0.58 +0.0 6,484,050 - -1.1 6,484,505 + Pf6N2E2_5671 0.44 +0.2 6,484,506 - Pf6N2E2_5671 0.44 -0.7 6,484,668 - Pf6N2E2_5671 0.60 -0.8 6,484,673 + Pf6N2E2_5671 0.60 +2.6 6,484,674 - Pf6N2E2_5671 0.61 -0.0 6,484,674 - Pf6N2E2_5671 0.61 +0.5
Or see this region's nucleotide sequence