Experiment: 4-Hydroxybenzoic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2829 and Pf6N2E2_2830 are separated by 10 nucleotides Pf6N2E2_2830 and Pf6N2E2_2831 are separated by 12 nucleotides Pf6N2E2_2831 and Pf6N2E2_2832 are separated by 152 nucleotides
Pf6N2E2_2829 - 4-carboxymuconolactone decarboxylase (EC 4.1.1.44), at 3,318,565 to 3,318,957
_2829
Pf6N2E2_2830 - Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24), at 3,318,968 to 3,319,768
_2830
Pf6N2E2_2831 - 3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2), at 3,319,781 to 3,321,145
_2831
Pf6N2E2_2832 - dicarboxylic acid transporter PcaT, at 3,321,298 to 3,322,596
_2832
Position (kb)
3319
3320
3321
3322 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3318.919 kb on + strand at 3318.920 kb on - strand at 3318.938 kb on + strand at 3318.938 kb on + strand at 3319.054 kb on - strand, within Pf6N2E2_2830 at 3319.065 kb on + strand, within Pf6N2E2_2830 at 3319.331 kb on + strand, within Pf6N2E2_2830 at 3319.359 kb on - strand, within Pf6N2E2_2830 at 3319.431 kb on + strand, within Pf6N2E2_2830 at 3319.431 kb on + strand, within Pf6N2E2_2830 at 3319.431 kb on + strand, within Pf6N2E2_2830 at 3319.507 kb on + strand, within Pf6N2E2_2830 at 3319.507 kb on + strand, within Pf6N2E2_2830 at 3319.731 kb on + strand at 3319.731 kb on + strand at 3320.706 kb on + strand, within Pf6N2E2_2831 at 3320.706 kb on + strand, within Pf6N2E2_2831 at 3320.828 kb on + strand, within Pf6N2E2_2831 at 3320.828 kb on + strand, within Pf6N2E2_2831 at 3320.838 kb on + strand, within Pf6N2E2_2831 at 3320.839 kb on - strand, within Pf6N2E2_2831 at 3320.839 kb on - strand, within Pf6N2E2_2831 at 3320.839 kb on - strand, within Pf6N2E2_2831 at 3320.950 kb on - strand, within Pf6N2E2_2831 at 3321.108 kb on + strand at 3321.108 kb on + strand at 3321.109 kb on - strand at 3321.109 kb on - strand at 3321.109 kb on - strand at 3321.145 kb on + strand at 3321.145 kb on + strand at 3321.145 kb on + strand at 3321.165 kb on - strand at 3321.165 kb on - strand at 3321.165 kb on - strand at 3321.165 kb on - strand at 3321.165 kb on - strand at 3321.165 kb on - strand at 3321.165 kb on - strand at 3321.165 kb on - strand at 3321.317 kb on + strand at 3321.317 kb on + strand at 3321.380 kb on + strand at 3321.380 kb on + strand at 3321.380 kb on + strand at 3321.380 kb on + strand at 3321.381 kb on - strand at 3321.381 kb on - strand at 3321.381 kb on - strand at 3321.381 kb on - strand at 3321.431 kb on + strand, within Pf6N2E2_2832 at 3321.431 kb on + strand, within Pf6N2E2_2832 at 3321.431 kb on + strand, within Pf6N2E2_2832 at 3321.431 kb on + strand, within Pf6N2E2_2832 at 3321.432 kb on - strand, within Pf6N2E2_2832 at 3321.432 kb on - strand, within Pf6N2E2_2832 at 3321.432 kb on - strand, within Pf6N2E2_2832 at 3321.470 kb on + strand, within Pf6N2E2_2832 at 3321.471 kb on - strand, within Pf6N2E2_2832 at 3321.481 kb on + strand, within Pf6N2E2_2832 at 3321.481 kb on + strand, within Pf6N2E2_2832 at 3321.481 kb on + strand, within Pf6N2E2_2832 at 3321.482 kb on - strand, within Pf6N2E2_2832 at 3321.482 kb on - strand, within Pf6N2E2_2832 at 3321.482 kb on - strand, within Pf6N2E2_2832 at 3321.545 kb on + strand, within Pf6N2E2_2832 at 3321.545 kb on + strand, within Pf6N2E2_2832 at 3321.545 kb on + strand, within Pf6N2E2_2832 at 3321.546 kb on - strand, within Pf6N2E2_2832 at 3321.546 kb on - strand, within Pf6N2E2_2832 at 3321.546 kb on - strand, within Pf6N2E2_2832 at 3321.546 kb on - strand, within Pf6N2E2_2832 at 3321.546 kb on - strand, within Pf6N2E2_2832 at 3321.546 kb on - strand, within Pf6N2E2_2832 at 3321.676 kb on - strand, within Pf6N2E2_2832 at 3321.728 kb on + strand, within Pf6N2E2_2832 at 3321.728 kb on + strand, within Pf6N2E2_2832 at 3321.728 kb on + strand, within Pf6N2E2_2832 at 3321.728 kb on + strand, within Pf6N2E2_2832 at 3321.728 kb on + strand, within Pf6N2E2_2832 at 3321.728 kb on + strand, within Pf6N2E2_2832 at 3321.729 kb on - strand at 3321.729 kb on - strand, within Pf6N2E2_2832 at 3321.755 kb on + strand, within Pf6N2E2_2832 at 3321.755 kb on + strand, within Pf6N2E2_2832 at 3321.755 kb on + strand, within Pf6N2E2_2832 at 3321.756 kb on - strand, within Pf6N2E2_2832 at 3321.756 kb on - strand, within Pf6N2E2_2832 at 3321.756 kb on - strand, within Pf6N2E2_2832 at 3321.797 kb on + strand, within Pf6N2E2_2832 at 3321.797 kb on + strand, within Pf6N2E2_2832 at 3321.798 kb on - strand, within Pf6N2E2_2832 at 3321.798 kb on - strand, within Pf6N2E2_2832 at 3321.798 kb on - strand, within Pf6N2E2_2832 at 3321.809 kb on + strand, within Pf6N2E2_2832 at 3321.809 kb on + strand, within Pf6N2E2_2832 at 3321.809 kb on + strand, within Pf6N2E2_2832 at 3321.809 kb on + strand, within Pf6N2E2_2832 at 3321.809 kb on + strand, within Pf6N2E2_2832 at 3321.809 kb on + strand, within Pf6N2E2_2832 at 3321.809 kb on + strand, within Pf6N2E2_2832 at 3321.809 kb on + strand, within Pf6N2E2_2832 at 3321.809 kb on + strand, within Pf6N2E2_2832 at 3321.809 kb on + strand, within Pf6N2E2_2832 at 3321.809 kb on + strand, within Pf6N2E2_2832 at 3321.810 kb on - strand, within Pf6N2E2_2832 at 3321.810 kb on - strand, within Pf6N2E2_2832 at 3321.810 kb on - strand, within Pf6N2E2_2832 at 3321.810 kb on - strand, within Pf6N2E2_2832 at 3322.022 kb on + strand, within Pf6N2E2_2832 at 3322.023 kb on - strand, within Pf6N2E2_2832 at 3322.023 kb on - strand, within Pf6N2E2_2832 at 3322.109 kb on + strand, within Pf6N2E2_2832 at 3322.109 kb on + strand, within Pf6N2E2_2832
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Hydroxybenzoic Acid (C) remove 3,318,919 + -3.7 3,318,920 - -1.8 3,318,938 + -3.2 3,318,938 + -1.0 3,319,054 - Pf6N2E2_2830 0.11 -2.1 3,319,065 + Pf6N2E2_2830 0.12 +0.0 3,319,331 + Pf6N2E2_2830 0.45 -2.7 3,319,359 - Pf6N2E2_2830 0.49 -3.0 3,319,431 + Pf6N2E2_2830 0.58 -2.9 3,319,431 + Pf6N2E2_2830 0.58 -3.4 3,319,431 + Pf6N2E2_2830 0.58 -3.1 3,319,507 + Pf6N2E2_2830 0.67 -1.2 3,319,507 + Pf6N2E2_2830 0.67 -2.1 3,319,731 + -3.0 3,319,731 + -3.6 3,320,706 + Pf6N2E2_2831 0.68 -0.1 3,320,706 + Pf6N2E2_2831 0.68 -3.2 3,320,828 + Pf6N2E2_2831 0.77 -2.0 3,320,828 + Pf6N2E2_2831 0.77 -3.0 3,320,838 + Pf6N2E2_2831 0.77 -2.9 3,320,839 - Pf6N2E2_2831 0.78 -3.8 3,320,839 - Pf6N2E2_2831 0.78 -3.0 3,320,839 - Pf6N2E2_2831 0.78 -3.5 3,320,950 - Pf6N2E2_2831 0.86 -1.5 3,321,108 + -3.5 3,321,108 + -0.7 3,321,109 - -4.5 3,321,109 - -0.5 3,321,109 - -4.4 3,321,145 + -3.0 3,321,145 + -4.1 3,321,145 + -2.3 3,321,165 - +0.4 3,321,165 - +0.7 3,321,165 - +1.4 3,321,165 - +0.6 3,321,165 - -0.3 3,321,165 - +1.4 3,321,165 - -0.5 3,321,165 - +0.6 3,321,317 + -3.0 3,321,317 + -4.5 3,321,380 + -2.1 3,321,380 + -2.0 3,321,380 + -2.9 3,321,380 + -4.1 3,321,381 - -0.4 3,321,381 - +0.5 3,321,381 - -0.4 3,321,381 - -2.2 3,321,431 + Pf6N2E2_2832 0.10 -2.1 3,321,431 + Pf6N2E2_2832 0.10 -1.7 3,321,431 + Pf6N2E2_2832 0.10 -1.8 3,321,431 + Pf6N2E2_2832 0.10 -4.1 3,321,432 - Pf6N2E2_2832 0.10 -1.5 3,321,432 - Pf6N2E2_2832 0.10 +0.0 3,321,432 - Pf6N2E2_2832 0.10 -0.5 3,321,470 + Pf6N2E2_2832 0.13 -3.2 3,321,471 - Pf6N2E2_2832 0.13 +1.0 3,321,481 + Pf6N2E2_2832 0.14 -4.5 3,321,481 + Pf6N2E2_2832 0.14 -2.1 3,321,481 + Pf6N2E2_2832 0.14 -4.0 3,321,482 - Pf6N2E2_2832 0.14 +1.0 3,321,482 - Pf6N2E2_2832 0.14 +0.8 3,321,482 - Pf6N2E2_2832 0.14 +0.2 3,321,545 + Pf6N2E2_2832 0.19 -3.7 3,321,545 + Pf6N2E2_2832 0.19 -3.3 3,321,545 + Pf6N2E2_2832 0.19 -3.2 3,321,546 - Pf6N2E2_2832 0.19 -1.3 3,321,546 - Pf6N2E2_2832 0.19 -0.9 3,321,546 - Pf6N2E2_2832 0.19 +1.0 3,321,546 - Pf6N2E2_2832 0.19 -0.5 3,321,546 - Pf6N2E2_2832 0.19 -0.2 3,321,546 - Pf6N2E2_2832 0.19 -0.6 3,321,676 - Pf6N2E2_2832 0.29 +0.9 3,321,728 + Pf6N2E2_2832 0.33 -3.9 3,321,728 + Pf6N2E2_2832 0.33 -3.8 3,321,728 + Pf6N2E2_2832 0.33 -3.0 3,321,728 + Pf6N2E2_2832 0.33 -1.9 3,321,728 + Pf6N2E2_2832 0.33 -2.7 3,321,728 + Pf6N2E2_2832 0.33 -3.0 3,321,729 - -0.5 3,321,729 - Pf6N2E2_2832 0.33 -1.0 3,321,755 + Pf6N2E2_2832 0.35 -3.2 3,321,755 + Pf6N2E2_2832 0.35 -3.5 3,321,755 + Pf6N2E2_2832 0.35 -3.3 3,321,756 - Pf6N2E2_2832 0.35 -1.4 3,321,756 - Pf6N2E2_2832 0.35 -0.8 3,321,756 - Pf6N2E2_2832 0.35 -1.3 3,321,797 + Pf6N2E2_2832 0.38 -3.9 3,321,797 + Pf6N2E2_2832 0.38 -2.4 3,321,798 - Pf6N2E2_2832 0.38 -2.0 3,321,798 - Pf6N2E2_2832 0.38 +0.0 3,321,798 - Pf6N2E2_2832 0.38 -0.3 3,321,809 + Pf6N2E2_2832 0.39 -4.2 3,321,809 + Pf6N2E2_2832 0.39 -4.5 3,321,809 + Pf6N2E2_2832 0.39 -3.7 3,321,809 + Pf6N2E2_2832 0.39 -4.2 3,321,809 + Pf6N2E2_2832 0.39 -2.0 3,321,809 + Pf6N2E2_2832 0.39 -2.3 3,321,809 + Pf6N2E2_2832 0.39 -1.5 3,321,809 + Pf6N2E2_2832 0.39 -3.1 3,321,809 + Pf6N2E2_2832 0.39 -2.8 3,321,809 + Pf6N2E2_2832 0.39 -1.4 3,321,809 + Pf6N2E2_2832 0.39 -3.0 3,321,810 - Pf6N2E2_2832 0.39 -2.6 3,321,810 - Pf6N2E2_2832 0.39 +2.0 3,321,810 - Pf6N2E2_2832 0.39 -0.2 3,321,810 - Pf6N2E2_2832 0.39 +0.0 3,322,022 + Pf6N2E2_2832 0.56 -2.0 3,322,023 - Pf6N2E2_2832 0.56 -1.1 3,322,023 - Pf6N2E2_2832 0.56 +0.3 3,322,109 + Pf6N2E2_2832 0.62 -4.3 3,322,109 + Pf6N2E2_2832 0.62 -2.0
Or see this region's nucleotide sequence