Experiment: 4-Hydroxybenzoic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2828 and Pf6N2E2_2829 are separated by 206 nucleotides Pf6N2E2_2829 and Pf6N2E2_2830 are separated by 10 nucleotides Pf6N2E2_2830 and Pf6N2E2_2831 are separated by 12 nucleotides
Pf6N2E2_2828 - PhaK-like protein, at 3,317,105 to 3,318,358
_2828
Pf6N2E2_2829 - 4-carboxymuconolactone decarboxylase (EC 4.1.1.44), at 3,318,565 to 3,318,957
_2829
Pf6N2E2_2830 - Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24), at 3,318,968 to 3,319,768
_2830
Pf6N2E2_2831 - 3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2), at 3,319,781 to 3,321,145
_2831
Position (kb)
3318
3319 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3317.621 kb on + strand, within Pf6N2E2_2828 at 3317.621 kb on + strand, within Pf6N2E2_2828 at 3317.622 kb on - strand, within Pf6N2E2_2828 at 3317.622 kb on - strand, within Pf6N2E2_2828 at 3317.622 kb on - strand, within Pf6N2E2_2828 at 3317.736 kb on - strand, within Pf6N2E2_2828 at 3317.736 kb on - strand, within Pf6N2E2_2828 at 3318.050 kb on + strand, within Pf6N2E2_2828 at 3318.050 kb on + strand, within Pf6N2E2_2828 at 3318.050 kb on + strand, within Pf6N2E2_2828 at 3318.050 kb on + strand, within Pf6N2E2_2828 at 3318.050 kb on + strand, within Pf6N2E2_2828 at 3318.050 kb on + strand, within Pf6N2E2_2828 at 3318.062 kb on + strand, within Pf6N2E2_2828 at 3318.093 kb on + strand, within Pf6N2E2_2828 at 3318.093 kb on + strand, within Pf6N2E2_2828 at 3318.094 kb on - strand, within Pf6N2E2_2828 at 3318.094 kb on - strand, within Pf6N2E2_2828 at 3318.153 kb on - strand, within Pf6N2E2_2828 at 3318.227 kb on + strand, within Pf6N2E2_2828 at 3318.228 kb on - strand, within Pf6N2E2_2828 at 3318.296 kb on + strand at 3318.296 kb on + strand at 3318.297 kb on - strand at 3318.301 kb on + strand at 3318.301 kb on + strand at 3318.302 kb on - strand at 3318.302 kb on - strand at 3318.302 kb on - strand at 3318.356 kb on + strand at 3318.356 kb on + strand at 3318.356 kb on + strand at 3318.357 kb on - strand at 3318.357 kb on - strand at 3318.383 kb on - strand at 3318.405 kb on + strand at 3318.405 kb on + strand at 3318.463 kb on + strand at 3318.463 kb on + strand at 3318.463 kb on + strand at 3318.463 kb on + strand at 3318.464 kb on - strand at 3318.647 kb on + strand, within Pf6N2E2_2829 at 3318.647 kb on + strand, within Pf6N2E2_2829 at 3318.647 kb on + strand, within Pf6N2E2_2829 at 3318.648 kb on - strand, within Pf6N2E2_2829 at 3318.648 kb on - strand, within Pf6N2E2_2829 at 3318.648 kb on - strand, within Pf6N2E2_2829 at 3318.768 kb on + strand, within Pf6N2E2_2829 at 3318.769 kb on - strand, within Pf6N2E2_2829 at 3318.769 kb on - strand, within Pf6N2E2_2829 at 3318.919 kb on + strand at 3318.920 kb on - strand at 3318.938 kb on + strand at 3318.938 kb on + strand at 3319.054 kb on - strand, within Pf6N2E2_2830 at 3319.065 kb on + strand, within Pf6N2E2_2830 at 3319.331 kb on + strand, within Pf6N2E2_2830 at 3319.359 kb on - strand, within Pf6N2E2_2830 at 3319.431 kb on + strand, within Pf6N2E2_2830 at 3319.431 kb on + strand, within Pf6N2E2_2830 at 3319.431 kb on + strand, within Pf6N2E2_2830 at 3319.507 kb on + strand, within Pf6N2E2_2830 at 3319.507 kb on + strand, within Pf6N2E2_2830 at 3319.731 kb on + strand at 3319.731 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Hydroxybenzoic Acid (C) remove 3,317,621 + Pf6N2E2_2828 0.41 +0.8 3,317,621 + Pf6N2E2_2828 0.41 -0.6 3,317,622 - Pf6N2E2_2828 0.41 -0.5 3,317,622 - Pf6N2E2_2828 0.41 -1.2 3,317,622 - Pf6N2E2_2828 0.41 -0.5 3,317,736 - Pf6N2E2_2828 0.50 +0.8 3,317,736 - Pf6N2E2_2828 0.50 +0.0 3,318,050 + Pf6N2E2_2828 0.75 +0.7 3,318,050 + Pf6N2E2_2828 0.75 -3.5 3,318,050 + Pf6N2E2_2828 0.75 +0.6 3,318,050 + Pf6N2E2_2828 0.75 +0.5 3,318,050 + Pf6N2E2_2828 0.75 +0.5 3,318,050 + Pf6N2E2_2828 0.75 -0.4 3,318,062 + Pf6N2E2_2828 0.76 -1.1 3,318,093 + Pf6N2E2_2828 0.79 -0.5 3,318,093 + Pf6N2E2_2828 0.79 +0.6 3,318,094 - Pf6N2E2_2828 0.79 +0.4 3,318,094 - Pf6N2E2_2828 0.79 +0.2 3,318,153 - Pf6N2E2_2828 0.84 +0.3 3,318,227 + Pf6N2E2_2828 0.89 +0.8 3,318,228 - Pf6N2E2_2828 0.90 +0.4 3,318,296 + -0.2 3,318,296 + -0.1 3,318,297 - -0.3 3,318,301 + -0.3 3,318,301 + +0.6 3,318,302 - +1.3 3,318,302 - +2.3 3,318,302 - -0.5 3,318,356 + -0.5 3,318,356 + +0.3 3,318,356 + -1.0 3,318,357 - +0.4 3,318,357 - -0.2 3,318,383 - +1.5 3,318,405 + -0.6 3,318,405 + +0.0 3,318,463 + +0.1 3,318,463 + -0.6 3,318,463 + +1.7 3,318,463 + +0.5 3,318,464 - -0.0 3,318,647 + Pf6N2E2_2829 0.21 -1.0 3,318,647 + Pf6N2E2_2829 0.21 -3.1 3,318,647 + Pf6N2E2_2829 0.21 -2.7 3,318,648 - Pf6N2E2_2829 0.21 -2.8 3,318,648 - Pf6N2E2_2829 0.21 -2.7 3,318,648 - Pf6N2E2_2829 0.21 -2.3 3,318,768 + Pf6N2E2_2829 0.52 -3.7 3,318,769 - Pf6N2E2_2829 0.52 -2.4 3,318,769 - Pf6N2E2_2829 0.52 -1.5 3,318,919 + -3.7 3,318,920 - -1.8 3,318,938 + -3.2 3,318,938 + -1.0 3,319,054 - Pf6N2E2_2830 0.11 -2.1 3,319,065 + Pf6N2E2_2830 0.12 +0.0 3,319,331 + Pf6N2E2_2830 0.45 -2.7 3,319,359 - Pf6N2E2_2830 0.49 -3.0 3,319,431 + Pf6N2E2_2830 0.58 -2.9 3,319,431 + Pf6N2E2_2830 0.58 -3.4 3,319,431 + Pf6N2E2_2830 0.58 -3.1 3,319,507 + Pf6N2E2_2830 0.67 -1.2 3,319,507 + Pf6N2E2_2830 0.67 -2.1 3,319,731 + -3.0 3,319,731 + -3.6
Or see this region's nucleotide sequence