Experiment: 4-Hydroxybenzoic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2287 and Pf6N2E2_2288 are separated by 115 nucleotides Pf6N2E2_2288 and Pf6N2E2_2289 are separated by 108 nucleotides Pf6N2E2_2289 and Pf6N2E2_2290 are separated by 54 nucleotides
Pf6N2E2_2287 - DNA topoisomerase I (EC 5.99.1.2), at 2,704,969 to 2,707,581
_2287
Pf6N2E2_2288 - FIG00953423: hypothetical protein, at 2,707,697 to 2,707,933
_2288
Pf6N2E2_2289 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 2,708,042 to 2,709,193
_2289
Pf6N2E2_2290 - Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 2,709,248 to 2,711,395
_2290
Position (kb)
2708
2709
2710 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2707.644 kb on - strand at 2707.644 kb on - strand at 2707.644 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.650 kb on - strand at 2707.650 kb on - strand at 2707.650 kb on - strand at 2707.650 kb on - strand at 2707.833 kb on + strand, within Pf6N2E2_2288 at 2707.880 kb on + strand, within Pf6N2E2_2288 at 2707.902 kb on + strand, within Pf6N2E2_2288 at 2707.902 kb on + strand, within Pf6N2E2_2288 at 2707.903 kb on - strand, within Pf6N2E2_2288 at 2707.903 kb on - strand, within Pf6N2E2_2288 at 2707.903 kb on - strand, within Pf6N2E2_2288 at 2707.903 kb on - strand, within Pf6N2E2_2288 at 2707.911 kb on + strand at 2707.925 kb on + strand at 2707.925 kb on + strand at 2707.926 kb on - strand at 2707.982 kb on - strand at 2707.984 kb on + strand at 2707.985 kb on - strand at 2707.985 kb on - strand at 2708.043 kb on + strand at 2708.044 kb on - strand at 2708.044 kb on - strand at 2708.362 kb on - strand, within Pf6N2E2_2289 at 2708.791 kb on - strand, within Pf6N2E2_2289 at 2709.203 kb on - strand at 2709.217 kb on + strand at 2709.218 kb on - strand at 2709.218 kb on - strand at 2709.218 kb on - strand at 2709.218 kb on - strand at 2709.218 kb on - strand at 2709.218 kb on - strand at 2709.376 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Hydroxybenzoic Acid (C) remove 2,707,644 - -0.1 2,707,644 - +1.3 2,707,644 - +1.0 2,707,646 - -0.1 2,707,646 - +1.4 2,707,646 - +0.2 2,707,646 - -1.2 2,707,646 - +0.9 2,707,646 - +0.7 2,707,646 - +0.6 2,707,646 - +1.7 2,707,646 - +0.5 2,707,646 - +2.5 2,707,646 - +0.9 2,707,646 - -0.1 2,707,646 - -0.2 2,707,646 - -1.3 2,707,646 - +0.2 2,707,646 - +0.6 2,707,646 - -0.8 2,707,646 - +0.3 2,707,646 - +1.2 2,707,646 - +1.4 2,707,648 - +1.3 2,707,648 - +0.2 2,707,648 - -0.0 2,707,648 - +0.6 2,707,648 - +1.4 2,707,648 - -0.8 2,707,648 - +1.9 2,707,648 - -0.2 2,707,648 - -1.9 2,707,648 - -0.6 2,707,648 - +0.0 2,707,648 - -0.5 2,707,648 - +0.3 2,707,648 - +1.7 2,707,648 - +0.7 2,707,650 - -0.2 2,707,650 - +0.4 2,707,650 - -0.3 2,707,650 - +0.2 2,707,833 + Pf6N2E2_2288 0.57 -0.8 2,707,880 + Pf6N2E2_2288 0.77 -0.6 2,707,902 + Pf6N2E2_2288 0.86 -1.3 2,707,902 + Pf6N2E2_2288 0.86 -0.8 2,707,903 - Pf6N2E2_2288 0.87 +0.4 2,707,903 - Pf6N2E2_2288 0.87 +2.1 2,707,903 - Pf6N2E2_2288 0.87 +1.5 2,707,903 - Pf6N2E2_2288 0.87 -0.2 2,707,911 + -0.5 2,707,925 + +1.0 2,707,925 + -1.5 2,707,926 - -0.3 2,707,982 - -0.2 2,707,984 + +0.6 2,707,985 - -1.6 2,707,985 - +0.9 2,708,043 + +0.9 2,708,044 - -0.1 2,708,044 - +0.3 2,708,362 - Pf6N2E2_2289 0.28 -1.2 2,708,791 - Pf6N2E2_2289 0.65 -1.3 2,709,203 - -4.2 2,709,217 + -2.5 2,709,218 - -1.3 2,709,218 - -2.6 2,709,218 - -2.1 2,709,218 - -2.1 2,709,218 - +1.4 2,709,218 - -0.2 2,709,376 - +0.7
Or see this region's nucleotide sequence