Experiment: 4-Hydroxybenzoic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1830 and Pf6N2E2_1831 are separated by 17 nucleotides Pf6N2E2_1831 and Pf6N2E2_1832 overlap by 4 nucleotides Pf6N2E2_1832 and Pf6N2E2_1833 overlap by 8 nucleotides
Pf6N2E2_1830 - Methylsuccinyl-CoA dehydrogenase, predicted by (Erb et al, 2007), at 2,167,717 to 2,169,387
_1830
Pf6N2E2_1831 - Citrate lyase beta chain (EC 4.1.3.6), at 2,169,405 to 2,170,304
_1831
Pf6N2E2_1832 - COGs COG3777, at 2,170,301 to 2,171,158
_1832
Pf6N2E2_1833 - 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / Enoyl CoA hydratase (EC 4.2.1.17), at 2,171,151 to 2,172,668
_1833
Position (kb)
2169
2170
2171 Strain fitness (log2 ratio)
-2
-1
0
1 at 2168.415 kb on + strand, within Pf6N2E2_1830 at 2168.419 kb on - strand, within Pf6N2E2_1830 at 2168.434 kb on + strand, within Pf6N2E2_1830 at 2168.434 kb on + strand, within Pf6N2E2_1830 at 2168.543 kb on + strand, within Pf6N2E2_1830 at 2168.836 kb on - strand, within Pf6N2E2_1830 at 2169.019 kb on + strand, within Pf6N2E2_1830 at 2169.019 kb on + strand, within Pf6N2E2_1830 at 2169.019 kb on + strand, within Pf6N2E2_1830 at 2169.020 kb on - strand, within Pf6N2E2_1830 at 2169.165 kb on + strand, within Pf6N2E2_1830 at 2169.245 kb on - strand at 2169.245 kb on - strand at 2169.387 kb on + strand at 2169.387 kb on + strand at 2169.387 kb on + strand at 2169.387 kb on + strand at 2169.388 kb on - strand at 2169.388 kb on - strand at 2169.410 kb on + strand at 2169.411 kb on - strand at 2169.411 kb on - strand at 2169.888 kb on + strand, within Pf6N2E2_1831 at 2169.904 kb on - strand, within Pf6N2E2_1831 at 2169.904 kb on - strand, within Pf6N2E2_1831 at 2170.088 kb on + strand, within Pf6N2E2_1831 at 2170.088 kb on + strand, within Pf6N2E2_1831 at 2170.088 kb on + strand, within Pf6N2E2_1831 at 2170.104 kb on + strand, within Pf6N2E2_1831 at 2170.104 kb on + strand, within Pf6N2E2_1831 at 2170.105 kb on - strand, within Pf6N2E2_1831 at 2170.105 kb on - strand, within Pf6N2E2_1831 at 2170.253 kb on + strand at 2170.406 kb on + strand, within Pf6N2E2_1832 at 2170.407 kb on - strand, within Pf6N2E2_1832 at 2170.407 kb on - strand, within Pf6N2E2_1832 at 2170.407 kb on - strand, within Pf6N2E2_1832 at 2170.463 kb on + strand, within Pf6N2E2_1832 at 2170.500 kb on - strand, within Pf6N2E2_1832 at 2170.572 kb on + strand, within Pf6N2E2_1832 at 2170.649 kb on - strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.904 kb on - strand, within Pf6N2E2_1832 at 2170.904 kb on - strand, within Pf6N2E2_1832 at 2170.996 kb on - strand, within Pf6N2E2_1832 at 2171.036 kb on - strand, within Pf6N2E2_1832 at 2171.036 kb on - strand, within Pf6N2E2_1832 at 2171.136 kb on + strand at 2171.136 kb on + strand at 2171.136 kb on + strand at 2171.137 kb on - strand at 2171.181 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Hydroxybenzoic Acid (C) remove 2,168,415 + Pf6N2E2_1830 0.42 +0.8 2,168,419 - Pf6N2E2_1830 0.42 -0.5 2,168,434 + Pf6N2E2_1830 0.43 -0.5 2,168,434 + Pf6N2E2_1830 0.43 -1.6 2,168,543 + Pf6N2E2_1830 0.49 -0.7 2,168,836 - Pf6N2E2_1830 0.67 -0.1 2,169,019 + Pf6N2E2_1830 0.78 -0.4 2,169,019 + Pf6N2E2_1830 0.78 -0.5 2,169,019 + Pf6N2E2_1830 0.78 +0.1 2,169,020 - Pf6N2E2_1830 0.78 -1.6 2,169,165 + Pf6N2E2_1830 0.87 +0.5 2,169,245 - +0.3 2,169,245 - -0.9 2,169,387 + -0.4 2,169,387 + +1.3 2,169,387 + +0.7 2,169,387 + -0.4 2,169,388 - +0.3 2,169,388 - -0.5 2,169,410 + +0.2 2,169,411 - -1.3 2,169,411 - +1.5 2,169,888 + Pf6N2E2_1831 0.54 -0.8 2,169,904 - Pf6N2E2_1831 0.55 -0.1 2,169,904 - Pf6N2E2_1831 0.55 -1.7 2,170,088 + Pf6N2E2_1831 0.76 -0.1 2,170,088 + Pf6N2E2_1831 0.76 -2.1 2,170,088 + Pf6N2E2_1831 0.76 -1.2 2,170,104 + Pf6N2E2_1831 0.78 -1.5 2,170,104 + Pf6N2E2_1831 0.78 +1.0 2,170,105 - Pf6N2E2_1831 0.78 -1.7 2,170,105 - Pf6N2E2_1831 0.78 -0.9 2,170,253 + -0.1 2,170,406 + Pf6N2E2_1832 0.12 +0.2 2,170,407 - Pf6N2E2_1832 0.12 +1.1 2,170,407 - Pf6N2E2_1832 0.12 +0.3 2,170,407 - Pf6N2E2_1832 0.12 +1.5 2,170,463 + Pf6N2E2_1832 0.19 -0.9 2,170,500 - Pf6N2E2_1832 0.23 -0.9 2,170,572 + Pf6N2E2_1832 0.32 +0.5 2,170,649 - Pf6N2E2_1832 0.41 +0.1 2,170,903 + Pf6N2E2_1832 0.70 +0.4 2,170,903 + Pf6N2E2_1832 0.70 -0.2 2,170,903 + Pf6N2E2_1832 0.70 +0.8 2,170,903 + Pf6N2E2_1832 0.70 -0.2 2,170,903 + Pf6N2E2_1832 0.70 +1.5 2,170,903 + Pf6N2E2_1832 0.70 -0.9 2,170,903 + Pf6N2E2_1832 0.70 +0.4 2,170,904 - Pf6N2E2_1832 0.70 -0.3 2,170,904 - Pf6N2E2_1832 0.70 -0.1 2,170,996 - Pf6N2E2_1832 0.81 -1.3 2,171,036 - Pf6N2E2_1832 0.86 +0.7 2,171,036 - Pf6N2E2_1832 0.86 -0.9 2,171,136 + -0.9 2,171,136 + -0.3 2,171,136 + -0.0 2,171,137 - +0.6 2,171,181 + +0.3
Or see this region's nucleotide sequence