Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1727

Experiment: 4-Hydroxybenzoic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1726 and Pf6N2E2_1727 are separated by 162 nucleotidesPf6N2E2_1727 and Pf6N2E2_1728 are separated by 126 nucleotides Pf6N2E2_1726 - 3-methylmercaptopropionyl-CoA ligase (DmdB), at 2,034,356 to 2,035,978 _1726 Pf6N2E2_1727 - Acyl-CoA dehydrogenase (EC 1.3.8.7), at 2,036,141 to 2,037,913 _1727 Pf6N2E2_1728 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 2,038,040 to 2,041,051 _1728 Position (kb) 2036 2037 2038Strain fitness (log2 ratio) -2 -1 0 1 2at 2035.331 kb on + strand, within Pf6N2E2_1726at 2035.438 kb on - strand, within Pf6N2E2_1726at 2035.438 kb on - strand, within Pf6N2E2_1726at 2035.438 kb on - strand, within Pf6N2E2_1726at 2035.643 kb on + strand, within Pf6N2E2_1726at 2035.825 kb on + strandat 2035.825 kb on + strandat 2035.826 kb on - strandat 2036.390 kb on + strand, within Pf6N2E2_1727at 2036.390 kb on + strand, within Pf6N2E2_1727at 2036.399 kb on + strand, within Pf6N2E2_1727at 2036.399 kb on + strand, within Pf6N2E2_1727at 2036.399 kb on + strand, within Pf6N2E2_1727at 2036.476 kb on - strand, within Pf6N2E2_1727at 2036.495 kb on + strand, within Pf6N2E2_1727at 2036.495 kb on + strand, within Pf6N2E2_1727at 2036.496 kb on - strand, within Pf6N2E2_1727at 2036.591 kb on + strand, within Pf6N2E2_1727at 2036.606 kb on + strand, within Pf6N2E2_1727at 2036.607 kb on - strand, within Pf6N2E2_1727at 2037.023 kb on + strand, within Pf6N2E2_1727at 2037.180 kb on + strand, within Pf6N2E2_1727at 2037.181 kb on - strand, within Pf6N2E2_1727at 2037.240 kb on - strand, within Pf6N2E2_1727at 2037.388 kb on - strand, within Pf6N2E2_1727at 2037.404 kb on + strand, within Pf6N2E2_1727at 2037.405 kb on - strand, within Pf6N2E2_1727at 2037.444 kb on + strand, within Pf6N2E2_1727at 2037.445 kb on - strand, within Pf6N2E2_1727at 2037.445 kb on - strand, within Pf6N2E2_1727at 2037.461 kb on + strand, within Pf6N2E2_1727at 2037.462 kb on - strand, within Pf6N2E2_1727at 2037.462 kb on - strand, within Pf6N2E2_1727at 2037.462 kb on - strand, within Pf6N2E2_1727at 2037.462 kb on - strand, within Pf6N2E2_1727at 2037.714 kb on - strand, within Pf6N2E2_1727at 2037.729 kb on - strand, within Pf6N2E2_1727at 2037.781 kb on + strandat 2037.781 kb on + strandat 2037.781 kb on + strandat 2037.782 kb on - strandat 2037.782 kb on - strandat 2037.818 kb on + strandat 2038.141 kb on + strandat 2038.141 kb on + strandat 2038.411 kb on - strand, within Pf6N2E2_1728at 2038.530 kb on + strand, within Pf6N2E2_1728at 2038.530 kb on + strand, within Pf6N2E2_1728at 2038.678 kb on + strand, within Pf6N2E2_1728at 2038.836 kb on + strand, within Pf6N2E2_1728at 2038.913 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 4-Hydroxybenzoic Acid (C)
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2,035,331 + Pf6N2E2_1726 0.60 -0.5
2,035,438 - Pf6N2E2_1726 0.67 -1.6
2,035,438 - Pf6N2E2_1726 0.67 +0.2
2,035,438 - Pf6N2E2_1726 0.67 +0.7
2,035,643 + Pf6N2E2_1726 0.79 -1.6
2,035,825 + +1.4
2,035,825 + -0.4
2,035,826 - -0.4
2,036,390 + Pf6N2E2_1727 0.14 +0.1
2,036,390 + Pf6N2E2_1727 0.14 +1.0
2,036,399 + Pf6N2E2_1727 0.15 +0.8
2,036,399 + Pf6N2E2_1727 0.15 -0.3
2,036,399 + Pf6N2E2_1727 0.15 -0.8
2,036,476 - Pf6N2E2_1727 0.19 -1.0
2,036,495 + Pf6N2E2_1727 0.20 -1.6
2,036,495 + Pf6N2E2_1727 0.20 +1.6
2,036,496 - Pf6N2E2_1727 0.20 -0.5
2,036,591 + Pf6N2E2_1727 0.25 +0.0
2,036,606 + Pf6N2E2_1727 0.26 +1.4
2,036,607 - Pf6N2E2_1727 0.26 -0.6
2,037,023 + Pf6N2E2_1727 0.50 +1.1
2,037,180 + Pf6N2E2_1727 0.59 -0.1
2,037,181 - Pf6N2E2_1727 0.59 +0.2
2,037,240 - Pf6N2E2_1727 0.62 +0.4
2,037,388 - Pf6N2E2_1727 0.70 +1.0
2,037,404 + Pf6N2E2_1727 0.71 +1.8
2,037,405 - Pf6N2E2_1727 0.71 -0.6
2,037,444 + Pf6N2E2_1727 0.73 +0.4
2,037,445 - Pf6N2E2_1727 0.74 +0.2
2,037,445 - Pf6N2E2_1727 0.74 +0.7
2,037,461 + Pf6N2E2_1727 0.74 +0.3
2,037,462 - Pf6N2E2_1727 0.75 +0.6
2,037,462 - Pf6N2E2_1727 0.75 +0.7
2,037,462 - Pf6N2E2_1727 0.75 +0.1
2,037,462 - Pf6N2E2_1727 0.75 -0.9
2,037,714 - Pf6N2E2_1727 0.89 +0.3
2,037,729 - Pf6N2E2_1727 0.90 -2.0
2,037,781 + +0.3
2,037,781 + +0.9
2,037,781 + +0.2
2,037,782 - -2.4
2,037,782 - +0.8
2,037,818 + +0.6
2,038,141 + -0.2
2,038,141 + +0.2
2,038,411 - Pf6N2E2_1728 0.12 -1.2
2,038,530 + Pf6N2E2_1728 0.16 +0.4
2,038,530 + Pf6N2E2_1728 0.16 +0.2
2,038,678 + Pf6N2E2_1728 0.21 +0.6
2,038,836 + Pf6N2E2_1728 0.26 +0.4
2,038,913 - -0.6

Or see this region's nucleotide sequence