Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25995 and BWI76_RS26000 overlap by 11 nucleotides BWI76_RS26000 and BWI76_RS26005 are separated by 89 nucleotides BWI76_RS26005 and BWI76_RS26010 are separated by 31 nucleotides
BWI76_RS25995: BWI76_RS25995 - membrane protein, at 5,371,779 to 5,373,857
_RS25995
BWI76_RS26000: BWI76_RS26000 - aspartate aminotransferase family protein, at 5,373,847 to 5,375,067
_RS26000
BWI76_RS26005: BWI76_RS26005 - glutamine amidotransferase, at 5,375,157 to 5,375,720
_RS26005
BWI76_RS26010: BWI76_RS26010 - cell filamentation protein Fic, at 5,375,752 to 5,376,354
_RS26010
Position (kb) 5373
5374
5375
5376 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5372.885 kb on + strand, within BWI76_RS25995 at 5372.890 kb on + strand, within BWI76_RS25995 at 5372.890 kb on + strand, within BWI76_RS25995 at 5372.921 kb on - strand, within BWI76_RS25995 at 5372.936 kb on + strand, within BWI76_RS25995 at 5372.944 kb on - strand, within BWI76_RS25995 at 5373.073 kb on - strand, within BWI76_RS25995 at 5373.086 kb on - strand, within BWI76_RS25995 at 5373.093 kb on - strand, within BWI76_RS25995 at 5373.105 kb on - strand, within BWI76_RS25995 at 5373.110 kb on - strand, within BWI76_RS25995 at 5373.159 kb on - strand, within BWI76_RS25995 at 5373.174 kb on - strand, within BWI76_RS25995 at 5373.183 kb on + strand, within BWI76_RS25995 at 5373.372 kb on - strand, within BWI76_RS25995 at 5373.545 kb on + strand, within BWI76_RS25995 at 5373.553 kb on - strand, within BWI76_RS25995 at 5373.569 kb on - strand, within BWI76_RS25995 at 5373.618 kb on - strand, within BWI76_RS25995 at 5373.930 kb on + strand at 5373.948 kb on - strand at 5374.067 kb on + strand, within BWI76_RS26000 at 5374.262 kb on + strand, within BWI76_RS26000 at 5374.302 kb on + strand, within BWI76_RS26000 at 5374.312 kb on + strand, within BWI76_RS26000 at 5374.312 kb on + strand, within BWI76_RS26000 at 5374.315 kb on - strand, within BWI76_RS26000 at 5374.396 kb on + strand, within BWI76_RS26000 at 5374.418 kb on - strand, within BWI76_RS26000 at 5374.475 kb on - strand, within BWI76_RS26000 at 5374.649 kb on + strand, within BWI76_RS26000 at 5374.823 kb on - strand, within BWI76_RS26000 at 5374.970 kb on + strand at 5374.978 kb on - strand at 5375.294 kb on + strand, within BWI76_RS26005 at 5375.367 kb on - strand, within BWI76_RS26005 at 5375.781 kb on - strand at 5375.781 kb on - strand at 5375.850 kb on - strand, within BWI76_RS26010 at 5375.870 kb on - strand, within BWI76_RS26010 at 5375.982 kb on - strand, within BWI76_RS26010 at 5376.018 kb on + strand, within BWI76_RS26010
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x remove 5,372,885 + BWI76_RS25995 0.53 -0.3 5,372,890 + BWI76_RS25995 0.53 +0.6 5,372,890 + BWI76_RS25995 0.53 -0.5 5,372,921 - BWI76_RS25995 0.55 -0.1 5,372,936 + BWI76_RS25995 0.56 -0.0 5,372,944 - BWI76_RS25995 0.56 -0.6 5,373,073 - BWI76_RS25995 0.62 -0.6 5,373,086 - BWI76_RS25995 0.63 +0.2 5,373,093 - BWI76_RS25995 0.63 -0.4 5,373,105 - BWI76_RS25995 0.64 +0.2 5,373,110 - BWI76_RS25995 0.64 +0.0 5,373,159 - BWI76_RS25995 0.66 -0.4 5,373,174 - BWI76_RS25995 0.67 +0.3 5,373,183 + BWI76_RS25995 0.68 -0.1 5,373,372 - BWI76_RS25995 0.77 -0.5 5,373,545 + BWI76_RS25995 0.85 -0.4 5,373,553 - BWI76_RS25995 0.85 +1.0 5,373,569 - BWI76_RS25995 0.86 +0.3 5,373,618 - BWI76_RS25995 0.88 +0.3 5,373,930 + +1.1 5,373,948 - -3.4 5,374,067 + BWI76_RS26000 0.18 -2.8 5,374,262 + BWI76_RS26000 0.34 -2.0 5,374,302 + BWI76_RS26000 0.37 -2.9 5,374,312 + BWI76_RS26000 0.38 -3.1 5,374,312 + BWI76_RS26000 0.38 -4.4 5,374,315 - BWI76_RS26000 0.38 -2.5 5,374,396 + BWI76_RS26000 0.45 -3.4 5,374,418 - BWI76_RS26000 0.47 -2.5 5,374,475 - BWI76_RS26000 0.51 -3.5 5,374,649 + BWI76_RS26000 0.66 -1.4 5,374,823 - BWI76_RS26000 0.80 -2.5 5,374,970 + -2.5 5,374,978 - -3.3 5,375,294 + BWI76_RS26005 0.24 -0.0 5,375,367 - BWI76_RS26005 0.37 +0.0 5,375,781 - +0.1 5,375,781 - -0.4 5,375,850 - BWI76_RS26010 0.16 +0.3 5,375,870 - BWI76_RS26010 0.20 -0.4 5,375,982 - BWI76_RS26010 0.38 +0.1 5,376,018 + BWI76_RS26010 0.44 +0.1
Or see this region's nucleotide sequence