Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20340 and BWI76_RS20345 are separated by 291 nucleotides BWI76_RS20345 and BWI76_RS20350 are separated by 136 nucleotides BWI76_RS20350 and BWI76_RS20355 are separated by 33 nucleotides BWI76_RS20355 and BWI76_RS20360 are separated by 199 nucleotides
BWI76_RS20340: BWI76_RS20340 - histidine ABC transporter substrate-binding protein HisJ, at 4,258,059 to 4,258,841
_RS20340
BWI76_RS20345: BWI76_RS20345 - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase, at 4,259,133 to 4,259,702
_RS20345
BWI76_RS20350: BWI76_RS20350 - amidophosphoribosyltransferase, at 4,259,839 to 4,261,356
_RS20350
BWI76_RS20355: BWI76_RS20355 - colicin V production protein, at 4,261,390 to 4,261,878
_RS20355
BWI76_RS20360: BWI76_RS20360 - cell division protein DedD, at 4,262,078 to 4,262,767
_RS20360
Position (kb) 4259
4260
4261
4262 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 4258.848 kb on + strand at 4259.966 kb on + strand at 4259.966 kb on + strand at 4260.090 kb on + strand, within BWI76_RS20350 at 4260.225 kb on + strand, within BWI76_RS20350 at 4260.314 kb on - strand, within BWI76_RS20350 at 4260.658 kb on + strand, within BWI76_RS20350 at 4260.664 kb on - strand, within BWI76_RS20350 at 4261.070 kb on - strand, within BWI76_RS20350 at 4261.323 kb on + strand at 4261.470 kb on + strand, within BWI76_RS20355 at 4261.551 kb on - strand, within BWI76_RS20355 at 4261.570 kb on + strand, within BWI76_RS20355 at 4262.158 kb on + strand, within BWI76_RS20360
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x remove 4,258,848 + +0.2 4,259,966 + -4.3 4,259,966 + -4.1 4,260,090 + BWI76_RS20350 0.17 -4.6 4,260,225 + BWI76_RS20350 0.25 -5.0 4,260,314 - BWI76_RS20350 0.31 -4.2 4,260,658 + BWI76_RS20350 0.54 -5.3 4,260,664 - BWI76_RS20350 0.54 -6.6 4,261,070 - BWI76_RS20350 0.81 -5.4 4,261,323 + -2.1 4,261,470 + BWI76_RS20355 0.16 -5.8 4,261,551 - BWI76_RS20355 0.33 -4.7 4,261,570 + BWI76_RS20355 0.37 -4.7 4,262,158 + BWI76_RS20360 0.12 -0.4
Or see this region's nucleotide sequence