Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16115 and BWI76_RS16120 are separated by 83 nucleotides BWI76_RS16120 and BWI76_RS16125 overlap by 11 nucleotides BWI76_RS16125 and BWI76_RS16130 overlap by 11 nucleotides
BWI76_RS16115: BWI76_RS16115 - fumarate hydratase, at 3,343,519 to 3,345,174
_RS16115
BWI76_RS16120: BWI76_RS16120 - 4-carboxymuconolactone decarboxylase, at 3,345,258 to 3,345,641
_RS16120
BWI76_RS16125: BWI76_RS16125 - 3-oxoadipate enol-lactonase, at 3,345,631 to 3,346,398
_RS16125
BWI76_RS16130: BWI76_RS16130 - 3-carboxy-cis,cis-muconate cycloisomerase, at 3,346,388 to 3,347,740
_RS16130
Position (kb)
3345
3346 Strain fitness (log2 ratio)
-1
0
1 at 3344.316 kb on - strand, within BWI76_RS16115 at 3344.316 kb on - strand, within BWI76_RS16115 at 3344.603 kb on + strand, within BWI76_RS16115 at 3344.637 kb on + strand, within BWI76_RS16115 at 3344.689 kb on - strand, within BWI76_RS16115 at 3344.746 kb on - strand, within BWI76_RS16115 at 3345.032 kb on + strand at 3345.032 kb on + strand at 3345.228 kb on + strand at 3345.392 kb on - strand, within BWI76_RS16120 at 3345.440 kb on + strand, within BWI76_RS16120 at 3345.585 kb on + strand, within BWI76_RS16120 at 3345.593 kb on - strand, within BWI76_RS16120 at 3345.654 kb on + strand at 3345.903 kb on + strand, within BWI76_RS16125 at 3346.488 kb on - strand at 3346.494 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x remove 3,344,316 - BWI76_RS16115 0.48 -0.2 3,344,316 - BWI76_RS16115 0.48 +0.1 3,344,603 + BWI76_RS16115 0.65 +0.2 3,344,637 + BWI76_RS16115 0.68 -0.0 3,344,689 - BWI76_RS16115 0.71 -0.3 3,344,746 - BWI76_RS16115 0.74 -0.6 3,345,032 + -0.2 3,345,032 + -0.2 3,345,228 + -0.3 3,345,392 - BWI76_RS16120 0.35 +0.1 3,345,440 + BWI76_RS16120 0.47 +0.1 3,345,585 + BWI76_RS16120 0.85 +0.6 3,345,593 - BWI76_RS16120 0.87 +0.3 3,345,654 + +0.4 3,345,903 + BWI76_RS16125 0.35 +0.7 3,346,488 - -1.0 3,346,494 + -0.1
Or see this region's nucleotide sequence