Experiment: library passed through aphids in food, 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3462 and Psyr_3463 are separated by 24 nucleotides Psyr_3463 and Psyr_3464 are separated by 123 nucleotides Psyr_3464 and Psyr_3465 are separated by 83 nucleotides Psyr_3465 and Psyr_3466 are separated by 72 nucleotides
Psyr_3462: Psyr_3462 - conserved hypothetical protein, at 4,126,855 to 4,127,151
_3462
Psyr_3463: Psyr_3463 - Flagellar protein FliS, at 4,127,176 to 4,127,565
_3463
Psyr_3464: Psyr_3464 - Flagellar hook-associated protein 2, N-terminal:Flagellar hook-associated 2, C-terminal:Flagellin hook IN, at 4,127,689 to 4,129,140
_3464
Psyr_3465: Psyr_3465 - Flagellar protein FlaG protein, at 4,129,224 to 4,129,616
_3465
Psyr_3466: Psyr_3466 - Flagellin, C-terminal:Flagellin, N-terminal, at 4,129,689 to 4,130,537
_3466
Position (kb)
4127
4128
4129
4130 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4126.742 kb on + strand at 4126.742 kb on + strand at 4126.743 kb on - strand at 4126.743 kb on - strand at 4126.743 kb on - strand at 4126.744 kb on + strand at 4126.744 kb on + strand at 4126.745 kb on - strand at 4126.745 kb on - strand at 4126.769 kb on + strand at 4126.783 kb on + strand at 4126.783 kb on + strand at 4126.783 kb on + strand at 4126.783 kb on + strand at 4126.784 kb on - strand at 4126.784 kb on - strand at 4126.849 kb on + strand at 4126.871 kb on + strand at 4126.871 kb on + strand at 4126.872 kb on - strand at 4126.872 kb on - strand at 4126.873 kb on + strand at 4126.873 kb on + strand at 4126.873 kb on + strand at 4126.873 kb on + strand at 4126.874 kb on - strand at 4126.874 kb on - strand at 4126.964 kb on + strand, within Psyr_3462 at 4126.964 kb on + strand, within Psyr_3462 at 4126.965 kb on - strand, within Psyr_3462 at 4126.975 kb on + strand, within Psyr_3462 at 4127.152 kb on + strand at 4127.177 kb on + strand at 4127.294 kb on + strand, within Psyr_3463 at 4127.294 kb on + strand, within Psyr_3463 at 4127.294 kb on + strand, within Psyr_3463 at 4127.294 kb on + strand, within Psyr_3463 at 4127.294 kb on + strand, within Psyr_3463 at 4127.294 kb on + strand, within Psyr_3463 at 4127.295 kb on - strand, within Psyr_3463 at 4127.295 kb on - strand, within Psyr_3463 at 4127.295 kb on - strand, within Psyr_3463 at 4127.295 kb on - strand, within Psyr_3463 at 4127.295 kb on - strand, within Psyr_3463 at 4127.295 kb on - strand, within Psyr_3463 at 4127.552 kb on - strand at 4127.591 kb on + strand at 4127.591 kb on + strand at 4127.606 kb on + strand at 4127.636 kb on - strand at 4127.672 kb on - strand at 4127.733 kb on + strand at 4127.733 kb on + strand at 4127.733 kb on + strand at 4127.733 kb on + strand at 4127.733 kb on + strand at 4127.734 kb on - strand at 4127.734 kb on - strand at 4127.734 kb on - strand at 4127.734 kb on - strand at 4127.785 kb on - strand at 4127.785 kb on - strand at 4127.786 kb on + strand at 4127.786 kb on + strand at 4127.786 kb on + strand at 4127.786 kb on + strand at 4127.787 kb on - strand at 4127.787 kb on - strand at 4127.787 kb on - strand at 4127.818 kb on - strand at 4127.820 kb on + strand at 4127.820 kb on + strand at 4127.821 kb on - strand at 4127.821 kb on - strand at 4127.868 kb on + strand, within Psyr_3464 at 4127.869 kb on - strand, within Psyr_3464 at 4127.871 kb on + strand, within Psyr_3464 at 4127.872 kb on - strand, within Psyr_3464 at 4127.872 kb on - strand, within Psyr_3464 at 4127.872 kb on - strand, within Psyr_3464 at 4127.914 kb on - strand, within Psyr_3464 at 4128.130 kb on - strand, within Psyr_3464 at 4128.206 kb on + strand, within Psyr_3464 at 4128.206 kb on + strand, within Psyr_3464 at 4128.206 kb on + strand, within Psyr_3464 at 4128.206 kb on + strand, within Psyr_3464 at 4128.206 kb on + strand, within Psyr_3464 at 4128.207 kb on - strand, within Psyr_3464 at 4128.207 kb on - strand, within Psyr_3464 at 4128.210 kb on + strand, within Psyr_3464 at 4128.210 kb on + strand, within Psyr_3464 at 4128.211 kb on - strand, within Psyr_3464 at 4128.269 kb on + strand, within Psyr_3464 at 4128.269 kb on + strand, within Psyr_3464 at 4128.269 kb on + strand, within Psyr_3464 at 4128.269 kb on + strand, within Psyr_3464 at 4128.269 kb on + strand, within Psyr_3464 at 4128.270 kb on - strand, within Psyr_3464 at 4128.270 kb on - strand, within Psyr_3464 at 4128.528 kb on + strand, within Psyr_3464 at 4128.529 kb on - strand, within Psyr_3464 at 4128.529 kb on - strand, within Psyr_3464 at 4128.529 kb on - strand, within Psyr_3464 at 4128.719 kb on + strand, within Psyr_3464 at 4128.719 kb on + strand, within Psyr_3464 at 4128.719 kb on + strand, within Psyr_3464 at 4128.719 kb on + strand, within Psyr_3464 at 4128.720 kb on - strand, within Psyr_3464 at 4128.720 kb on - strand, within Psyr_3464 at 4128.763 kb on - strand, within Psyr_3464 at 4128.763 kb on - strand, within Psyr_3464 at 4128.763 kb on - strand, within Psyr_3464 at 4128.763 kb on - strand, within Psyr_3464 at 4128.891 kb on + strand, within Psyr_3464 at 4128.891 kb on + strand, within Psyr_3464 at 4128.891 kb on + strand, within Psyr_3464 at 4128.892 kb on - strand, within Psyr_3464 at 4128.892 kb on - strand, within Psyr_3464 at 4128.892 kb on - strand, within Psyr_3464 at 4128.892 kb on - strand, within Psyr_3464 at 4128.892 kb on - strand, within Psyr_3464 at 4128.900 kb on + strand, within Psyr_3464 at 4128.900 kb on + strand, within Psyr_3464 at 4128.901 kb on - strand, within Psyr_3464 at 4128.901 kb on - strand, within Psyr_3464 at 4129.111 kb on - strand at 4129.119 kb on + strand at 4129.119 kb on + strand at 4129.119 kb on + strand at 4129.119 kb on + strand at 4129.119 kb on + strand at 4129.119 kb on + strand at 4129.120 kb on - strand at 4129.120 kb on - strand at 4129.120 kb on - strand at 4129.120 kb on - strand at 4129.120 kb on - strand at 4129.120 kb on - strand at 4129.125 kb on + strand at 4129.125 kb on + strand at 4129.125 kb on + strand at 4129.126 kb on - strand at 4129.126 kb on - strand at 4129.126 kb on - strand at 4129.126 kb on - strand at 4129.126 kb on - strand at 4129.308 kb on + strand, within Psyr_3465 at 4129.308 kb on + strand, within Psyr_3465 at 4129.308 kb on + strand, within Psyr_3465 at 4129.308 kb on + strand, within Psyr_3465 at 4129.309 kb on - strand, within Psyr_3465 at 4129.311 kb on + strand, within Psyr_3465 at 4129.311 kb on + strand, within Psyr_3465 at 4129.311 kb on + strand, within Psyr_3465 at 4129.311 kb on + strand, within Psyr_3465 at 4129.312 kb on - strand, within Psyr_3465 at 4129.312 kb on - strand, within Psyr_3465 at 4129.312 kb on - strand, within Psyr_3465 at 4129.312 kb on - strand, within Psyr_3465 at 4129.439 kb on + strand, within Psyr_3465 at 4129.439 kb on + strand, within Psyr_3465 at 4129.482 kb on - strand, within Psyr_3465 at 4129.482 kb on - strand, within Psyr_3465 at 4129.482 kb on - strand, within Psyr_3465 at 4129.482 kb on - strand, within Psyr_3465 at 4129.611 kb on - strand at 4129.657 kb on + strand at 4129.657 kb on + strand at 4129.658 kb on - strand at 4129.658 kb on - strand at 4129.658 kb on - strand at 4129.690 kb on + strand at 4129.691 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 24 hours remove 4,126,742 + +0.6 4,126,742 + -0.5 4,126,743 - +0.7 4,126,743 - +0.5 4,126,743 - +0.3 4,126,744 + +1.1 4,126,744 + +0.7 4,126,745 - +0.0 4,126,745 - -1.3 4,126,769 + -1.4 4,126,783 + -1.5 4,126,783 + -0.2 4,126,783 + -1.2 4,126,783 + -0.1 4,126,784 - +0.1 4,126,784 - +0.1 4,126,849 + -0.4 4,126,871 + -1.8 4,126,871 + -1.4 4,126,872 - -1.4 4,126,872 - -2.7 4,126,873 + +0.1 4,126,873 + -0.4 4,126,873 + -0.3 4,126,873 + -1.5 4,126,874 - -0.7 4,126,874 - -0.2 4,126,964 + Psyr_3462 0.37 -1.7 4,126,964 + Psyr_3462 0.37 -1.6 4,126,965 - Psyr_3462 0.37 -2.8 4,126,975 + Psyr_3462 0.40 -2.3 4,127,152 + -2.5 4,127,177 + -2.9 4,127,294 + Psyr_3463 0.30 -1.5 4,127,294 + Psyr_3463 0.30 -1.2 4,127,294 + Psyr_3463 0.30 -1.1 4,127,294 + Psyr_3463 0.30 -0.5 4,127,294 + Psyr_3463 0.30 -2.4 4,127,294 + Psyr_3463 0.30 +0.1 4,127,295 - Psyr_3463 0.31 -1.2 4,127,295 - Psyr_3463 0.31 -0.1 4,127,295 - Psyr_3463 0.31 -0.7 4,127,295 - Psyr_3463 0.31 -1.3 4,127,295 - Psyr_3463 0.31 -1.2 4,127,295 - Psyr_3463 0.31 -1.8 4,127,552 - +0.6 4,127,591 + -1.2 4,127,591 + -1.2 4,127,606 + -0.8 4,127,636 - +0.4 4,127,672 - +0.0 4,127,733 + +0.3 4,127,733 + -1.4 4,127,733 + -0.2 4,127,733 + -0.4 4,127,733 + -0.9 4,127,734 - +0.1 4,127,734 - -0.7 4,127,734 - -0.7 4,127,734 - +0.5 4,127,785 - -0.9 4,127,785 - -1.1 4,127,786 + -1.6 4,127,786 + -1.5 4,127,786 + -2.6 4,127,786 + -2.5 4,127,787 - -2.2 4,127,787 - -1.4 4,127,787 - -2.0 4,127,818 - -0.7 4,127,820 + +0.7 4,127,820 + -0.4 4,127,821 - -1.3 4,127,821 - -1.7 4,127,868 + Psyr_3464 0.12 -0.7 4,127,869 - Psyr_3464 0.12 -0.8 4,127,871 + Psyr_3464 0.13 -1.6 4,127,872 - Psyr_3464 0.13 -1.7 4,127,872 - Psyr_3464 0.13 -1.5 4,127,872 - Psyr_3464 0.13 -1.7 4,127,914 - Psyr_3464 0.15 -2.2 4,128,130 - Psyr_3464 0.30 -1.0 4,128,206 + Psyr_3464 0.36 -1.3 4,128,206 + Psyr_3464 0.36 -2.8 4,128,206 + Psyr_3464 0.36 -1.1 4,128,206 + Psyr_3464 0.36 -0.4 4,128,206 + Psyr_3464 0.36 -1.9 4,128,207 - Psyr_3464 0.36 -0.6 4,128,207 - Psyr_3464 0.36 -2.7 4,128,210 + Psyr_3464 0.36 -1.5 4,128,210 + Psyr_3464 0.36 -2.4 4,128,211 - Psyr_3464 0.36 -1.9 4,128,269 + Psyr_3464 0.40 -2.5 4,128,269 + Psyr_3464 0.40 -1.6 4,128,269 + Psyr_3464 0.40 -1.1 4,128,269 + Psyr_3464 0.40 +0.4 4,128,269 + Psyr_3464 0.40 -1.1 4,128,270 - Psyr_3464 0.40 -1.6 4,128,270 - Psyr_3464 0.40 -3.9 4,128,528 + Psyr_3464 0.58 -0.7 4,128,529 - Psyr_3464 0.58 -1.6 4,128,529 - Psyr_3464 0.58 -2.3 4,128,529 - Psyr_3464 0.58 -1.9 4,128,719 + Psyr_3464 0.71 -1.9 4,128,719 + Psyr_3464 0.71 -2.1 4,128,719 + Psyr_3464 0.71 -2.7 4,128,719 + Psyr_3464 0.71 -1.9 4,128,720 - Psyr_3464 0.71 -2.6 4,128,720 - Psyr_3464 0.71 -1.9 4,128,763 - Psyr_3464 0.74 -2.3 4,128,763 - Psyr_3464 0.74 -1.4 4,128,763 - Psyr_3464 0.74 -0.3 4,128,763 - Psyr_3464 0.74 -1.7 4,128,891 + Psyr_3464 0.83 -1.3 4,128,891 + Psyr_3464 0.83 -1.4 4,128,891 + Psyr_3464 0.83 +0.6 4,128,892 - Psyr_3464 0.83 -2.5 4,128,892 - Psyr_3464 0.83 -1.7 4,128,892 - Psyr_3464 0.83 -1.1 4,128,892 - Psyr_3464 0.83 +0.1 4,128,892 - Psyr_3464 0.83 -1.5 4,128,900 + Psyr_3464 0.83 -0.6 4,128,900 + Psyr_3464 0.83 +0.6 4,128,901 - Psyr_3464 0.83 -0.8 4,128,901 - Psyr_3464 0.83 -1.6 4,129,111 - -1.8 4,129,119 + -0.5 4,129,119 + -1.1 4,129,119 + -1.9 4,129,119 + -1.7 4,129,119 + +0.4 4,129,119 + -1.4 4,129,120 - -1.2 4,129,120 - -1.1 4,129,120 - -1.2 4,129,120 - -1.2 4,129,120 - -0.9 4,129,120 - +0.3 4,129,125 + -0.9 4,129,125 + -0.3 4,129,125 + -0.9 4,129,126 - -0.7 4,129,126 - -3.0 4,129,126 - -0.5 4,129,126 - -1.5 4,129,126 - +0.1 4,129,308 + Psyr_3465 0.21 -0.7 4,129,308 + Psyr_3465 0.21 -0.2 4,129,308 + Psyr_3465 0.21 -1.7 4,129,308 + Psyr_3465 0.21 -1.8 4,129,309 - Psyr_3465 0.22 +1.1 4,129,311 + Psyr_3465 0.22 +0.6 4,129,311 + Psyr_3465 0.22 -0.1 4,129,311 + Psyr_3465 0.22 -1.2 4,129,311 + Psyr_3465 0.22 +0.3 4,129,312 - Psyr_3465 0.22 +0.7 4,129,312 - Psyr_3465 0.22 +0.1 4,129,312 - Psyr_3465 0.22 +0.2 4,129,312 - Psyr_3465 0.22 -0.2 4,129,439 + Psyr_3465 0.55 +1.3 4,129,439 + Psyr_3465 0.55 +0.8 4,129,482 - Psyr_3465 0.66 -0.0 4,129,482 - Psyr_3465 0.66 +0.4 4,129,482 - Psyr_3465 0.66 +0.3 4,129,482 - Psyr_3465 0.66 +0.5 4,129,611 - +1.9 4,129,657 + +0.1 4,129,657 + +0.2 4,129,658 - -1.2 4,129,658 - -1.4 4,129,658 - +0.4 4,129,690 + -2.3 4,129,691 - -2.9
Or see this region's nucleotide sequence