Experiment: library passed through aphids in food, 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3437 and Psyr_3438 overlap by 4 nucleotides Psyr_3438 and Psyr_3439 are separated by 115 nucleotides Psyr_3439 and Psyr_3440 are separated by 11 nucleotides
Psyr_3437: Psyr_3437 - RNA polymerase, sigma 28 subunit, SigD/FliA/WhiG, at 4,100,576 to 4,101,316
_3437
Psyr_3438: Psyr_3438 - Cobyrinic acid a,c-diamide synthase, at 4,101,313 to 4,102,146
_3438
Psyr_3439: Psyr_3439 - GTP-binding signal recognition particle SRP54, G-domain protein, at 4,102,262 to 4,103,590
_3439
Psyr_3440: Psyr_3440 - Flagellar biosynthesis protein FlhA, at 4,103,602 to 4,105,731
_3440
Position (kb)
4102
4103
4104 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4101.264 kb on + strand at 4101.291 kb on + strand at 4101.291 kb on + strand at 4101.291 kb on + strand at 4101.292 kb on - strand at 4101.292 kb on - strand at 4101.292 kb on - strand at 4101.292 kb on - strand at 4101.523 kb on + strand, within Psyr_3438 at 4101.523 kb on + strand, within Psyr_3438 at 4101.523 kb on + strand, within Psyr_3438 at 4101.524 kb on - strand, within Psyr_3438 at 4102.149 kb on + strand at 4102.150 kb on - strand at 4102.150 kb on - strand at 4102.250 kb on - strand at 4102.253 kb on - strand at 4102.328 kb on - strand at 4102.388 kb on - strand at 4102.425 kb on + strand, within Psyr_3439 at 4102.425 kb on + strand, within Psyr_3439 at 4102.437 kb on - strand, within Psyr_3439 at 4102.437 kb on - strand, within Psyr_3439 at 4102.530 kb on + strand, within Psyr_3439 at 4102.531 kb on - strand, within Psyr_3439 at 4102.587 kb on + strand, within Psyr_3439 at 4102.587 kb on + strand, within Psyr_3439 at 4102.588 kb on - strand, within Psyr_3439 at 4102.588 kb on - strand, within Psyr_3439 at 4102.588 kb on - strand, within Psyr_3439 at 4102.829 kb on - strand, within Psyr_3439 at 4102.842 kb on + strand, within Psyr_3439 at 4102.843 kb on - strand, within Psyr_3439 at 4102.854 kb on + strand, within Psyr_3439 at 4102.855 kb on - strand, within Psyr_3439 at 4103.599 kb on - strand at 4103.635 kb on + strand at 4103.636 kb on - strand at 4103.735 kb on - strand at 4103.878 kb on + strand, within Psyr_3440 at 4103.879 kb on - strand, within Psyr_3440 at 4103.879 kb on - strand, within Psyr_3440 at 4103.932 kb on + strand, within Psyr_3440 at 4103.933 kb on - strand, within Psyr_3440 at 4104.058 kb on + strand, within Psyr_3440 at 4104.058 kb on + strand, within Psyr_3440 at 4104.058 kb on + strand, within Psyr_3440 at 4104.059 kb on - strand, within Psyr_3440 at 4104.215 kb on + strand, within Psyr_3440 at 4104.215 kb on + strand, within Psyr_3440 at 4104.269 kb on + strand, within Psyr_3440
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 24 hours remove 4,101,264 + -2.1 4,101,291 + -1.5 4,101,291 + -0.6 4,101,291 + -1.3 4,101,292 - -0.3 4,101,292 - -2.2 4,101,292 - -0.2 4,101,292 - -0.9 4,101,523 + Psyr_3438 0.25 +0.1 4,101,523 + Psyr_3438 0.25 -1.0 4,101,523 + Psyr_3438 0.25 +0.1 4,101,524 - Psyr_3438 0.25 -1.8 4,102,149 + +3.7 4,102,150 - -0.0 4,102,150 - +0.6 4,102,250 - -0.2 4,102,253 - -1.6 4,102,328 - -0.8 4,102,388 - -0.5 4,102,425 + Psyr_3439 0.12 -0.2 4,102,425 + Psyr_3439 0.12 -0.7 4,102,437 - Psyr_3439 0.13 -2.4 4,102,437 - Psyr_3439 0.13 -2.8 4,102,530 + Psyr_3439 0.20 +1.1 4,102,531 - Psyr_3439 0.20 -2.6 4,102,587 + Psyr_3439 0.24 -0.9 4,102,587 + Psyr_3439 0.24 +1.7 4,102,588 - Psyr_3439 0.25 -0.9 4,102,588 - Psyr_3439 0.25 -0.2 4,102,588 - Psyr_3439 0.25 -1.6 4,102,829 - Psyr_3439 0.43 -1.7 4,102,842 + Psyr_3439 0.44 +1.1 4,102,843 - Psyr_3439 0.44 -1.5 4,102,854 + Psyr_3439 0.45 -0.7 4,102,855 - Psyr_3439 0.45 -2.1 4,103,599 - -0.5 4,103,635 + +1.0 4,103,636 - -1.7 4,103,735 - +0.1 4,103,878 + Psyr_3440 0.13 +1.5 4,103,879 - Psyr_3440 0.13 -1.9 4,103,879 - Psyr_3440 0.13 -1.3 4,103,932 + Psyr_3440 0.15 -0.4 4,103,933 - Psyr_3440 0.16 -2.2 4,104,058 + Psyr_3440 0.21 -1.4 4,104,058 + Psyr_3440 0.21 -2.7 4,104,058 + Psyr_3440 0.21 -1.5 4,104,059 - Psyr_3440 0.21 -2.1 4,104,215 + Psyr_3440 0.29 -1.8 4,104,215 + Psyr_3440 0.29 -0.6 4,104,269 + Psyr_3440 0.31 -1.4
Or see this region's nucleotide sequence