Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_3439

Experiment: library passed through aphids in food, 24 hours

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_3437 and Psyr_3438 overlap by 4 nucleotidesPsyr_3438 and Psyr_3439 are separated by 115 nucleotidesPsyr_3439 and Psyr_3440 are separated by 11 nucleotides Psyr_3437: Psyr_3437 - RNA polymerase, sigma 28 subunit, SigD/FliA/WhiG, at 4,100,576 to 4,101,316 _3437 Psyr_3438: Psyr_3438 - Cobyrinic acid a,c-diamide synthase, at 4,101,313 to 4,102,146 _3438 Psyr_3439: Psyr_3439 - GTP-binding signal recognition particle SRP54, G-domain protein, at 4,102,262 to 4,103,590 _3439 Psyr_3440: Psyr_3440 - Flagellar biosynthesis protein FlhA, at 4,103,602 to 4,105,731 _3440 Position (kb) 4102 4103 4104Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 4101.264 kb on + strandat 4101.291 kb on + strandat 4101.291 kb on + strandat 4101.291 kb on + strandat 4101.292 kb on - strandat 4101.292 kb on - strandat 4101.292 kb on - strandat 4101.292 kb on - strandat 4101.523 kb on + strand, within Psyr_3438at 4101.523 kb on + strand, within Psyr_3438at 4101.523 kb on + strand, within Psyr_3438at 4101.524 kb on - strand, within Psyr_3438at 4102.149 kb on + strandat 4102.150 kb on - strandat 4102.150 kb on - strandat 4102.250 kb on - strandat 4102.253 kb on - strandat 4102.328 kb on - strandat 4102.388 kb on - strandat 4102.425 kb on + strand, within Psyr_3439at 4102.425 kb on + strand, within Psyr_3439at 4102.437 kb on - strand, within Psyr_3439at 4102.437 kb on - strand, within Psyr_3439at 4102.530 kb on + strand, within Psyr_3439at 4102.531 kb on - strand, within Psyr_3439at 4102.587 kb on + strand, within Psyr_3439at 4102.587 kb on + strand, within Psyr_3439at 4102.588 kb on - strand, within Psyr_3439at 4102.588 kb on - strand, within Psyr_3439at 4102.588 kb on - strand, within Psyr_3439at 4102.829 kb on - strand, within Psyr_3439at 4102.842 kb on + strand, within Psyr_3439at 4102.843 kb on - strand, within Psyr_3439at 4102.854 kb on + strand, within Psyr_3439at 4102.855 kb on - strand, within Psyr_3439at 4103.599 kb on - strandat 4103.635 kb on + strandat 4103.636 kb on - strandat 4103.735 kb on - strandat 4103.878 kb on + strand, within Psyr_3440at 4103.879 kb on - strand, within Psyr_3440at 4103.879 kb on - strand, within Psyr_3440at 4103.932 kb on + strand, within Psyr_3440at 4103.933 kb on - strand, within Psyr_3440at 4104.058 kb on + strand, within Psyr_3440at 4104.058 kb on + strand, within Psyr_3440at 4104.058 kb on + strand, within Psyr_3440at 4104.059 kb on - strand, within Psyr_3440at 4104.215 kb on + strand, within Psyr_3440at 4104.215 kb on + strand, within Psyr_3440at 4104.269 kb on + strand, within Psyr_3440

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Per-strain Table

Position Strand Gene LocusTag Fraction library passed through aphids in food, 24 hours
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4,101,264 + -2.1
4,101,291 + -1.5
4,101,291 + -0.6
4,101,291 + -1.3
4,101,292 - -0.3
4,101,292 - -2.2
4,101,292 - -0.2
4,101,292 - -0.9
4,101,523 + Psyr_3438 0.25 +0.1
4,101,523 + Psyr_3438 0.25 -1.0
4,101,523 + Psyr_3438 0.25 +0.1
4,101,524 - Psyr_3438 0.25 -1.8
4,102,149 + +3.7
4,102,150 - -0.0
4,102,150 - +0.6
4,102,250 - -0.2
4,102,253 - -1.6
4,102,328 - -0.8
4,102,388 - -0.5
4,102,425 + Psyr_3439 0.12 -0.2
4,102,425 + Psyr_3439 0.12 -0.7
4,102,437 - Psyr_3439 0.13 -2.4
4,102,437 - Psyr_3439 0.13 -2.8
4,102,530 + Psyr_3439 0.20 +1.1
4,102,531 - Psyr_3439 0.20 -2.6
4,102,587 + Psyr_3439 0.24 -0.9
4,102,587 + Psyr_3439 0.24 +1.7
4,102,588 - Psyr_3439 0.25 -0.9
4,102,588 - Psyr_3439 0.25 -0.2
4,102,588 - Psyr_3439 0.25 -1.6
4,102,829 - Psyr_3439 0.43 -1.7
4,102,842 + Psyr_3439 0.44 +1.1
4,102,843 - Psyr_3439 0.44 -1.5
4,102,854 + Psyr_3439 0.45 -0.7
4,102,855 - Psyr_3439 0.45 -2.1
4,103,599 - -0.5
4,103,635 + +1.0
4,103,636 - -1.7
4,103,735 - +0.1
4,103,878 + Psyr_3440 0.13 +1.5
4,103,879 - Psyr_3440 0.13 -1.9
4,103,879 - Psyr_3440 0.13 -1.3
4,103,932 + Psyr_3440 0.15 -0.4
4,103,933 - Psyr_3440 0.16 -2.2
4,104,058 + Psyr_3440 0.21 -1.4
4,104,058 + Psyr_3440 0.21 -2.7
4,104,058 + Psyr_3440 0.21 -1.5
4,104,059 - Psyr_3440 0.21 -2.1
4,104,215 + Psyr_3440 0.29 -1.8
4,104,215 + Psyr_3440 0.29 -0.6
4,104,269 + Psyr_3440 0.31 -1.4

Or see this region's nucleotide sequence