Experiment: library passed through aphids in food, 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2873 and Psyr_2874 are separated by 7 nucleotides Psyr_2874 and Psyr_2875 overlap by 4 nucleotides Psyr_2875 and Psyr_2876 overlap by 14 nucleotides
Psyr_2873: Psyr_2873 - Acyl-CoA dehydrogenase, C-terminal, at 3,447,102 to 3,448,340
_2873
Psyr_2874: Psyr_2874 - monosaccharide ABC transporter membrane protein, CUT2 family, at 3,448,348 to 3,449,334
_2874
Psyr_2875: Psyr_2875 - monosaccharide ABC transporter membrane protein, CUT2 family, at 3,449,331 to 3,450,335
_2875
Psyr_2876: Psyr_2876 - monosaccharide ABC transporter ATP-binding protein, CUT2 family, at 3,450,322 to 3,451,893
_2876
Position (kb)
3449
3450
3451 Strain fitness (log2 ratio)
-2
-1
0
1 at 3448.349 kb on + strand at 3448.349 kb on + strand at 3448.436 kb on + strand at 3448.703 kb on + strand, within Psyr_2874 at 3448.704 kb on - strand, within Psyr_2874 at 3448.706 kb on + strand, within Psyr_2874 at 3448.707 kb on - strand, within Psyr_2874 at 3448.707 kb on - strand, within Psyr_2874 at 3448.871 kb on + strand, within Psyr_2874 at 3448.872 kb on - strand, within Psyr_2874 at 3448.872 kb on - strand, within Psyr_2874 at 3448.872 kb on - strand, within Psyr_2874 at 3448.872 kb on - strand, within Psyr_2874 at 3448.872 kb on - strand, within Psyr_2874 at 3448.872 kb on - strand, within Psyr_2874 at 3449.104 kb on + strand at 3449.328 kb on + strand at 3449.493 kb on - strand, within Psyr_2875 at 3449.656 kb on + strand, within Psyr_2875 at 3449.656 kb on + strand, within Psyr_2875 at 3449.656 kb on + strand, within Psyr_2875 at 3449.656 kb on + strand, within Psyr_2875 at 3449.934 kb on + strand, within Psyr_2875 at 3450.318 kb on + strand at 3450.335 kb on + strand at 3450.614 kb on + strand, within Psyr_2876 at 3450.614 kb on + strand, within Psyr_2876 at 3450.614 kb on + strand, within Psyr_2876 at 3450.614 kb on + strand, within Psyr_2876 at 3450.614 kb on + strand, within Psyr_2876 at 3450.614 kb on + strand, within Psyr_2876 at 3450.615 kb on - strand, within Psyr_2876 at 3450.615 kb on - strand, within Psyr_2876 at 3450.616 kb on + strand, within Psyr_2876 at 3450.616 kb on + strand, within Psyr_2876 at 3450.616 kb on + strand, within Psyr_2876 at 3450.617 kb on - strand, within Psyr_2876 at 3451.127 kb on + strand, within Psyr_2876 at 3451.128 kb on - strand, within Psyr_2876 at 3451.265 kb on + strand, within Psyr_2876 at 3451.266 kb on - strand, within Psyr_2876 at 3451.277 kb on + strand, within Psyr_2876 at 3451.278 kb on - strand, within Psyr_2876 at 3451.278 kb on - strand, within Psyr_2876
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 24 hours remove 3,448,349 + +1.1 3,448,349 + -1.0 3,448,436 + +1.1 3,448,703 + Psyr_2874 0.36 +0.3 3,448,704 - Psyr_2874 0.36 +0.2 3,448,706 + Psyr_2874 0.36 +0.0 3,448,707 - Psyr_2874 0.36 +0.5 3,448,707 - Psyr_2874 0.36 -0.4 3,448,871 + Psyr_2874 0.53 +0.1 3,448,872 - Psyr_2874 0.53 +0.2 3,448,872 - Psyr_2874 0.53 +0.4 3,448,872 - Psyr_2874 0.53 +0.3 3,448,872 - Psyr_2874 0.53 +0.6 3,448,872 - Psyr_2874 0.53 -0.2 3,448,872 - Psyr_2874 0.53 -1.0 3,449,104 + -0.5 3,449,328 + -0.2 3,449,493 - Psyr_2875 0.16 -0.8 3,449,656 + Psyr_2875 0.32 -0.5 3,449,656 + Psyr_2875 0.32 -0.9 3,449,656 + Psyr_2875 0.32 -0.2 3,449,656 + Psyr_2875 0.32 -0.8 3,449,934 + Psyr_2875 0.60 -1.0 3,450,318 + +0.9 3,450,335 + -0.3 3,450,614 + Psyr_2876 0.19 +0.5 3,450,614 + Psyr_2876 0.19 -2.5 3,450,614 + Psyr_2876 0.19 -1.1 3,450,614 + Psyr_2876 0.19 +0.1 3,450,614 + Psyr_2876 0.19 +0.5 3,450,614 + Psyr_2876 0.19 -0.2 3,450,615 - Psyr_2876 0.19 +0.7 3,450,615 - Psyr_2876 0.19 +0.2 3,450,616 + Psyr_2876 0.19 +0.3 3,450,616 + Psyr_2876 0.19 +0.4 3,450,616 + Psyr_2876 0.19 +0.3 3,450,617 - Psyr_2876 0.19 +0.0 3,451,127 + Psyr_2876 0.51 +0.4 3,451,128 - Psyr_2876 0.51 -0.8 3,451,265 + Psyr_2876 0.60 +0.3 3,451,266 - Psyr_2876 0.60 +0.4 3,451,277 + Psyr_2876 0.61 -0.6 3,451,278 - Psyr_2876 0.61 +1.1 3,451,278 - Psyr_2876 0.61 +1.4
Or see this region's nucleotide sequence