Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_2223

Experiment: library passed through aphids in food, 24 hours

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_2222 and Psyr_2223 overlap by 4 nucleotidesPsyr_2223 and Psyr_2224 are separated by 13 nucleotides Psyr_2222: Psyr_2222 - aminomethyltransferase, at 2,582,408 to 2,585,314 _2222 Psyr_2223: Psyr_2223 - sarcosine oxidase, delta subunit, at 2,585,311 to 2,585,616 _2223 Psyr_2224: Psyr_2224 - FAD dependent oxidoreductase, at 2,585,630 to 2,586,871 _2224 Position (kb) 2585 2586Strain fitness (log2 ratio) -2 -1 0 1at 2584.360 kb on - strand, within Psyr_2222at 2584.413 kb on + strand, within Psyr_2222at 2584.414 kb on - strand, within Psyr_2222at 2584.537 kb on - strand, within Psyr_2222at 2584.567 kb on - strand, within Psyr_2222at 2584.567 kb on - strand, within Psyr_2222at 2584.567 kb on - strand, within Psyr_2222at 2584.567 kb on - strand, within Psyr_2222at 2584.696 kb on - strand, within Psyr_2222at 2584.696 kb on - strand, within Psyr_2222at 2584.824 kb on + strand, within Psyr_2222at 2584.825 kb on - strand, within Psyr_2222at 2584.825 kb on - strand, within Psyr_2222at 2584.825 kb on - strand, within Psyr_2222at 2584.825 kb on - strand, within Psyr_2222at 2584.840 kb on - strand, within Psyr_2222at 2584.929 kb on + strand, within Psyr_2222at 2584.929 kb on + strand, within Psyr_2222at 2584.930 kb on - strand, within Psyr_2222at 2584.945 kb on - strand, within Psyr_2222at 2585.069 kb on + strandat 2585.070 kb on - strandat 2585.148 kb on + strandat 2585.148 kb on + strandat 2585.148 kb on + strandat 2585.148 kb on + strandat 2585.226 kb on + strandat 2585.226 kb on + strandat 2585.498 kb on + strand, within Psyr_2223at 2585.499 kb on - strand, within Psyr_2223at 2585.808 kb on + strand, within Psyr_2224at 2585.808 kb on + strand, within Psyr_2224at 2585.811 kb on + strand, within Psyr_2224at 2585.812 kb on - strandat 2585.812 kb on - strand, within Psyr_2224at 2585.847 kb on + strand, within Psyr_2224at 2586.036 kb on + strand, within Psyr_2224at 2586.036 kb on + strand, within Psyr_2224at 2586.036 kb on + strand, within Psyr_2224at 2586.037 kb on - strand, within Psyr_2224at 2586.037 kb on - strand, within Psyr_2224at 2586.037 kb on - strand, within Psyr_2224at 2586.037 kb on - strand, within Psyr_2224at 2586.252 kb on - strand, within Psyr_2224at 2586.252 kb on - strand, within Psyr_2224at 2586.252 kb on - strand, within Psyr_2224at 2586.252 kb on - strand, within Psyr_2224at 2586.252 kb on - strand, within Psyr_2224at 2586.543 kb on + strand, within Psyr_2224at 2586.543 kb on + strand, within Psyr_2224at 2586.543 kb on + strand, within Psyr_2224at 2586.543 kb on + strand, within Psyr_2224at 2586.543 kb on + strand, within Psyr_2224at 2586.543 kb on + strand, within Psyr_2224at 2586.543 kb on + strand, within Psyr_2224at 2586.543 kb on + strand, within Psyr_2224at 2586.544 kb on - strand, within Psyr_2224at 2586.603 kb on + strand, within Psyr_2224at 2586.603 kb on + strand, within Psyr_2224at 2586.603 kb on + strand, within Psyr_2224at 2586.603 kb on + strand, within Psyr_2224at 2586.603 kb on + strand, within Psyr_2224at 2586.604 kb on - strand, within Psyr_2224at 2586.604 kb on - strand, within Psyr_2224

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Per-strain Table

Position Strand Gene LocusTag Fraction library passed through aphids in food, 24 hours
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2,584,360 - Psyr_2222 0.67 -0.9
2,584,413 + Psyr_2222 0.69 -0.6
2,584,414 - Psyr_2222 0.69 +1.0
2,584,537 - Psyr_2222 0.73 -0.3
2,584,567 - Psyr_2222 0.74 -0.3
2,584,567 - Psyr_2222 0.74 -0.0
2,584,567 - Psyr_2222 0.74 -0.2
2,584,567 - Psyr_2222 0.74 -1.3
2,584,696 - Psyr_2222 0.79 -0.3
2,584,696 - Psyr_2222 0.79 +0.8
2,584,824 + Psyr_2222 0.83 -0.6
2,584,825 - Psyr_2222 0.83 -0.3
2,584,825 - Psyr_2222 0.83 +0.1
2,584,825 - Psyr_2222 0.83 -0.4
2,584,825 - Psyr_2222 0.83 +0.2
2,584,840 - Psyr_2222 0.84 -0.8
2,584,929 + Psyr_2222 0.87 -1.2
2,584,929 + Psyr_2222 0.87 -0.2
2,584,930 - Psyr_2222 0.87 -1.2
2,584,945 - Psyr_2222 0.87 +0.1
2,585,069 + +0.0
2,585,070 - -0.3
2,585,148 + -0.5
2,585,148 + +0.1
2,585,148 + +0.2
2,585,148 + +0.3
2,585,226 + +0.3
2,585,226 + +0.4
2,585,498 + Psyr_2223 0.61 -0.7
2,585,499 - Psyr_2223 0.61 -2.4
2,585,808 + Psyr_2224 0.14 -0.7
2,585,808 + Psyr_2224 0.14 -1.1
2,585,811 + Psyr_2224 0.15 +0.1
2,585,812 - -1.5
2,585,812 - Psyr_2224 0.15 -0.5
2,585,847 + Psyr_2224 0.17 -0.8
2,586,036 + Psyr_2224 0.33 -0.5
2,586,036 + Psyr_2224 0.33 -0.6
2,586,036 + Psyr_2224 0.33 -0.3
2,586,037 - Psyr_2224 0.33 +0.2
2,586,037 - Psyr_2224 0.33 +0.1
2,586,037 - Psyr_2224 0.33 -0.0
2,586,037 - Psyr_2224 0.33 +0.7
2,586,252 - Psyr_2224 0.50 -0.2
2,586,252 - Psyr_2224 0.50 -0.3
2,586,252 - Psyr_2224 0.50 +0.3
2,586,252 - Psyr_2224 0.50 -0.6
2,586,252 - Psyr_2224 0.50 +0.9
2,586,543 + Psyr_2224 0.74 -0.0
2,586,543 + Psyr_2224 0.74 -1.4
2,586,543 + Psyr_2224 0.74 -0.4
2,586,543 + Psyr_2224 0.74 -2.0
2,586,543 + Psyr_2224 0.74 +0.1
2,586,543 + Psyr_2224 0.74 +0.3
2,586,543 + Psyr_2224 0.74 +1.1
2,586,543 + Psyr_2224 0.74 -0.0
2,586,544 - Psyr_2224 0.74 +0.4
2,586,603 + Psyr_2224 0.78 -1.1
2,586,603 + Psyr_2224 0.78 +1.1
2,586,603 + Psyr_2224 0.78 +0.1
2,586,603 + Psyr_2224 0.78 -0.1
2,586,603 + Psyr_2224 0.78 -1.8
2,586,604 - Psyr_2224 0.78 -0.1
2,586,604 - Psyr_2224 0.78 +0.9

Or see this region's nucleotide sequence