Experiment: library passed through aphids in food, 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1387 and Psyr_1388 are separated by 96 nucleotides Psyr_1388 and Psyr_1389 are separated by 10 nucleotides Psyr_1389 and Psyr_1390 are separated by 79 nucleotides Psyr_1390 and Psyr_1391 are separated by 92 nucleotides
Psyr_1387: Psyr_1387 - Ferredoxin--NADP(+) reductase, at 1,569,129 to 1,569,908
_1387
Psyr_1388: Psyr_1388 - Protein of unknown function UPF0066, at 1,570,005 to 1,570,697
_1388
Psyr_1389: Psyr_1389 - Protein of unknown function DUF1456, at 1,570,708 to 1,571,169
_1389
Psyr_1390: Psyr_1390 - RNA-binding S4, at 1,571,249 to 1,571,959
_1390
Psyr_1391: Psyr_1391 - GCN5-related N-acetyltransferase, at 1,572,052 to 1,572,492
_1391
Position (kb)
1570
1571
1572 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1569.906 kb on + strand at 1569.906 kb on + strand at 1569.907 kb on - strand at 1569.907 kb on - strand at 1569.907 kb on - strand at 1570.090 kb on + strand, within Psyr_1388 at 1570.300 kb on + strand, within Psyr_1388 at 1570.300 kb on + strand, within Psyr_1388 at 1570.300 kb on + strand, within Psyr_1388 at 1570.300 kb on + strand, within Psyr_1388 at 1570.300 kb on + strand, within Psyr_1388 at 1570.300 kb on + strand, within Psyr_1388 at 1570.301 kb on - strand, within Psyr_1388 at 1570.301 kb on - strand, within Psyr_1388 at 1570.301 kb on - strand, within Psyr_1388 at 1570.301 kb on - strand, within Psyr_1388 at 1570.301 kb on - strand, within Psyr_1388 at 1570.690 kb on + strand at 1570.690 kb on + strand at 1570.690 kb on + strand at 1570.690 kb on + strand at 1570.690 kb on + strand at 1570.690 kb on + strand at 1570.691 kb on - strand at 1570.691 kb on - strand at 1570.691 kb on - strand at 1570.691 kb on - strand at 1570.691 kb on - strand at 1570.691 kb on - strand at 1570.691 kb on - strand at 1570.775 kb on + strand, within Psyr_1389 at 1570.909 kb on - strand, within Psyr_1389 at 1570.974 kb on - strand, within Psyr_1389 at 1570.974 kb on - strand, within Psyr_1389 at 1570.974 kb on - strand, within Psyr_1389 at 1570.974 kb on - strand, within Psyr_1389 at 1570.974 kb on - strand, within Psyr_1389 at 1571.024 kb on + strand, within Psyr_1389 at 1571.024 kb on + strand, within Psyr_1389 at 1571.135 kb on + strand at 1571.135 kb on + strand at 1571.136 kb on - strand at 1571.136 kb on - strand at 1571.136 kb on - strand at 1571.152 kb on + strand at 1571.152 kb on + strand at 1571.152 kb on + strand at 1571.152 kb on + strand at 1571.152 kb on + strand at 1571.152 kb on + strand at 1571.153 kb on - strand at 1571.153 kb on - strand at 1571.153 kb on - strand at 1571.186 kb on + strand at 1571.186 kb on + strand at 1571.186 kb on + strand at 1571.186 kb on + strand at 1571.187 kb on - strand at 1571.226 kb on + strand at 1571.227 kb on - strand at 1571.227 kb on - strand at 1571.227 kb on - strand at 1571.229 kb on + strand at 1571.229 kb on + strand at 1571.230 kb on - strand at 1571.274 kb on + strand at 1571.275 kb on - strand at 1571.275 kb on - strand at 1571.466 kb on + strand, within Psyr_1390 at 1571.466 kb on + strand, within Psyr_1390 at 1571.466 kb on + strand, within Psyr_1390 at 1571.466 kb on + strand, within Psyr_1390 at 1571.466 kb on + strand, within Psyr_1390 at 1571.467 kb on - strand, within Psyr_1390 at 1571.467 kb on - strand, within Psyr_1390 at 1571.467 kb on - strand, within Psyr_1390 at 1571.532 kb on + strand, within Psyr_1390 at 1571.532 kb on + strand, within Psyr_1390 at 1571.532 kb on + strand, within Psyr_1390 at 1571.532 kb on + strand, within Psyr_1390 at 1571.533 kb on - strand, within Psyr_1390 at 1571.533 kb on - strand, within Psyr_1390 at 1571.533 kb on - strand, within Psyr_1390 at 1571.533 kb on - strand, within Psyr_1390 at 1571.679 kb on + strand, within Psyr_1390 at 1571.680 kb on - strand, within Psyr_1390 at 1571.680 kb on - strand, within Psyr_1390 at 1571.691 kb on - strand, within Psyr_1390 at 1571.978 kb on + strand at 1571.978 kb on + strand at 1571.998 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 24 hours remove 1,569,906 + -0.8 1,569,906 + +1.0 1,569,907 - +0.1 1,569,907 - +0.1 1,569,907 - +0.3 1,570,090 + Psyr_1388 0.12 -0.4 1,570,300 + Psyr_1388 0.43 -0.2 1,570,300 + Psyr_1388 0.43 +0.3 1,570,300 + Psyr_1388 0.43 -0.5 1,570,300 + Psyr_1388 0.43 -1.0 1,570,300 + Psyr_1388 0.43 -0.8 1,570,300 + Psyr_1388 0.43 +0.2 1,570,301 - Psyr_1388 0.43 -0.5 1,570,301 - Psyr_1388 0.43 +0.4 1,570,301 - Psyr_1388 0.43 +0.2 1,570,301 - Psyr_1388 0.43 +0.4 1,570,301 - Psyr_1388 0.43 +1.2 1,570,690 + -1.1 1,570,690 + -0.0 1,570,690 + -0.8 1,570,690 + +1.7 1,570,690 + +1.2 1,570,690 + -0.3 1,570,691 - -1.0 1,570,691 - +0.8 1,570,691 - +0.5 1,570,691 - -0.7 1,570,691 - -0.5 1,570,691 - +0.0 1,570,691 - -0.3 1,570,775 + Psyr_1389 0.15 -1.2 1,570,909 - Psyr_1389 0.44 -1.3 1,570,974 - Psyr_1389 0.58 -1.1 1,570,974 - Psyr_1389 0.58 -0.3 1,570,974 - Psyr_1389 0.58 -0.2 1,570,974 - Psyr_1389 0.58 -1.0 1,570,974 - Psyr_1389 0.58 -1.1 1,571,024 + Psyr_1389 0.68 -0.4 1,571,024 + Psyr_1389 0.68 +1.8 1,571,135 + +0.4 1,571,135 + -0.5 1,571,136 - +0.1 1,571,136 - +1.0 1,571,136 - +0.1 1,571,152 + -0.2 1,571,152 + +0.2 1,571,152 + +0.7 1,571,152 + +0.8 1,571,152 + +0.2 1,571,152 + -0.0 1,571,153 - -0.0 1,571,153 - +0.6 1,571,153 - +0.4 1,571,186 + -1.5 1,571,186 + -0.4 1,571,186 + -0.3 1,571,186 + -0.1 1,571,187 - -0.0 1,571,226 + +0.0 1,571,227 - +1.7 1,571,227 - +0.4 1,571,227 - -1.4 1,571,229 + -1.8 1,571,229 + +0.1 1,571,230 - +0.2 1,571,274 + -0.3 1,571,275 - +0.9 1,571,275 - +0.2 1,571,466 + Psyr_1390 0.31 +0.1 1,571,466 + Psyr_1390 0.31 +0.1 1,571,466 + Psyr_1390 0.31 -0.3 1,571,466 + Psyr_1390 0.31 -0.1 1,571,466 + Psyr_1390 0.31 -0.0 1,571,467 - Psyr_1390 0.31 -0.5 1,571,467 - Psyr_1390 0.31 -0.1 1,571,467 - Psyr_1390 0.31 -0.1 1,571,532 + Psyr_1390 0.40 +1.0 1,571,532 + Psyr_1390 0.40 +1.1 1,571,532 + Psyr_1390 0.40 -0.6 1,571,532 + Psyr_1390 0.40 +0.3 1,571,533 - Psyr_1390 0.40 +0.3 1,571,533 - Psyr_1390 0.40 -0.5 1,571,533 - Psyr_1390 0.40 +0.4 1,571,533 - Psyr_1390 0.40 -0.9 1,571,679 + Psyr_1390 0.60 -0.2 1,571,680 - Psyr_1390 0.61 +2.3 1,571,680 - Psyr_1390 0.61 +0.0 1,571,691 - Psyr_1390 0.62 -0.3 1,571,978 + -0.5 1,571,978 + -0.2 1,571,998 - +0.1
Or see this region's nucleotide sequence