Experiment: library passed through aphids in food, 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0560 and Psyr_0561 are separated by 207 nucleotides Psyr_0561 and Psyr_0562 are separated by 3 nucleotides
Psyr_0560: Psyr_0560 - conserved hypothetical protein, at 636,247 to 637,782
_0560
Psyr_0561: Psyr_0561 - chemotaxis motA protein, at 637,990 to 638,841
_0561
Psyr_0562: Psyr_0562 - OmpA/MotB, at 638,845 to 639,894
_0562
Position (kb)
637
638
639 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 637.146 kb on + strand, within Psyr_0560 at 637.147 kb on - strand, within Psyr_0560 at 637.709 kb on - strand at 637.771 kb on + strand at 637.771 kb on + strand at 637.772 kb on - strand at 637.772 kb on - strand at 637.772 kb on - strand at 637.805 kb on - strand at 637.805 kb on - strand at 637.859 kb on + strand at 637.859 kb on + strand at 637.860 kb on - strand at 637.860 kb on - strand at 637.871 kb on + strand at 637.871 kb on + strand at 637.871 kb on + strand at 637.871 kb on + strand at 637.872 kb on - strand at 637.872 kb on - strand at 637.872 kb on - strand at 637.899 kb on + strand at 637.900 kb on - strand at 637.900 kb on - strand at 637.913 kb on + strand at 637.979 kb on + strand at 637.990 kb on - strand at 637.996 kb on - strand at 638.041 kb on + strand at 638.042 kb on - strand at 638.084 kb on - strand, within Psyr_0561 at 638.143 kb on + strand, within Psyr_0561 at 638.143 kb on + strand, within Psyr_0561 at 638.143 kb on + strand, within Psyr_0561 at 638.143 kb on + strand, within Psyr_0561 at 638.143 kb on + strand, within Psyr_0561 at 638.144 kb on - strand, within Psyr_0561 at 638.144 kb on - strand, within Psyr_0561 at 638.144 kb on - strand, within Psyr_0561 at 638.206 kb on + strand, within Psyr_0561 at 638.206 kb on + strand, within Psyr_0561 at 638.206 kb on + strand, within Psyr_0561 at 638.206 kb on + strand, within Psyr_0561 at 638.206 kb on + strand, within Psyr_0561 at 638.207 kb on - strand, within Psyr_0561 at 638.207 kb on - strand, within Psyr_0561 at 638.207 kb on - strand, within Psyr_0561 at 638.207 kb on - strand, within Psyr_0561 at 638.207 kb on - strand, within Psyr_0561 at 638.207 kb on - strand, within Psyr_0561 at 638.207 kb on - strand, within Psyr_0561 at 638.209 kb on + strand, within Psyr_0561 at 638.210 kb on - strand, within Psyr_0561 at 638.210 kb on - strand, within Psyr_0561 at 638.210 kb on - strand, within Psyr_0561 at 638.210 kb on - strand, within Psyr_0561 at 638.359 kb on + strand, within Psyr_0561 at 638.359 kb on + strand, within Psyr_0561 at 638.359 kb on + strand, within Psyr_0561 at 638.359 kb on + strand, within Psyr_0561 at 638.359 kb on + strand, within Psyr_0561 at 638.359 kb on + strand, within Psyr_0561 at 638.360 kb on - strand, within Psyr_0561 at 638.360 kb on - strand, within Psyr_0561 at 638.360 kb on - strand, within Psyr_0561 at 638.360 kb on - strand, within Psyr_0561 at 638.360 kb on - strand, within Psyr_0561 at 638.499 kb on + strand, within Psyr_0561 at 638.594 kb on + strand, within Psyr_0561 at 638.594 kb on + strand, within Psyr_0561 at 638.594 kb on + strand, within Psyr_0561 at 638.594 kb on + strand, within Psyr_0561 at 638.594 kb on + strand, within Psyr_0561 at 638.594 kb on + strand, within Psyr_0561 at 638.595 kb on - strand, within Psyr_0561 at 638.595 kb on - strand, within Psyr_0561 at 638.595 kb on - strand, within Psyr_0561 at 638.595 kb on - strand, within Psyr_0561 at 638.595 kb on - strand, within Psyr_0561 at 638.595 kb on - strand, within Psyr_0561 at 638.595 kb on - strand, within Psyr_0561 at 638.595 kb on - strand, within Psyr_0561 at 638.786 kb on - strand at 638.839 kb on + strand at 638.854 kb on - strand at 639.061 kb on + strand, within Psyr_0562 at 639.062 kb on - strand, within Psyr_0562 at 639.374 kb on - strand, within Psyr_0562 at 639.598 kb on + strand, within Psyr_0562 at 639.598 kb on + strand, within Psyr_0562 at 639.598 kb on + strand, within Psyr_0562 at 639.598 kb on + strand, within Psyr_0562 at 639.598 kb on + strand, within Psyr_0562 at 639.599 kb on - strand, within Psyr_0562 at 639.599 kb on - strand, within Psyr_0562 at 639.814 kb on + strand at 639.814 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 24 hours remove 637,146 + Psyr_0560 0.59 -0.1 637,147 - Psyr_0560 0.59 -1.0 637,709 - +0.1 637,771 + +0.1 637,771 + -1.1 637,772 - -0.3 637,772 - -0.7 637,772 - -1.1 637,805 - -0.3 637,805 - -1.4 637,859 + +0.3 637,859 + +0.8 637,860 - -0.7 637,860 - -1.6 637,871 + +0.5 637,871 + -0.4 637,871 + +1.6 637,871 + -0.5 637,872 - -0.4 637,872 - -1.1 637,872 - -0.2 637,899 + -2.5 637,900 - -2.1 637,900 - -1.2 637,913 + -0.3 637,979 + -0.2 637,990 - -0.5 637,996 - -1.0 638,041 + +0.7 638,042 - -0.8 638,084 - Psyr_0561 0.11 -1.1 638,143 + Psyr_0561 0.18 -0.7 638,143 + Psyr_0561 0.18 -1.6 638,143 + Psyr_0561 0.18 -1.4 638,143 + Psyr_0561 0.18 +0.3 638,143 + Psyr_0561 0.18 -1.3 638,144 - Psyr_0561 0.18 -1.3 638,144 - Psyr_0561 0.18 -0.9 638,144 - Psyr_0561 0.18 -2.2 638,206 + Psyr_0561 0.25 -0.3 638,206 + Psyr_0561 0.25 -3.0 638,206 + Psyr_0561 0.25 -0.7 638,206 + Psyr_0561 0.25 -0.1 638,206 + Psyr_0561 0.25 -0.3 638,207 - Psyr_0561 0.25 -0.1 638,207 - Psyr_0561 0.25 -0.2 638,207 - Psyr_0561 0.25 -0.6 638,207 - Psyr_0561 0.25 -0.4 638,207 - Psyr_0561 0.25 -0.3 638,207 - Psyr_0561 0.25 -1.0 638,207 - Psyr_0561 0.25 -0.8 638,209 + Psyr_0561 0.26 -0.5 638,210 - Psyr_0561 0.26 -3.1 638,210 - Psyr_0561 0.26 -0.7 638,210 - Psyr_0561 0.26 -0.5 638,210 - Psyr_0561 0.26 -0.2 638,359 + Psyr_0561 0.43 -0.2 638,359 + Psyr_0561 0.43 -0.4 638,359 + Psyr_0561 0.43 -1.3 638,359 + Psyr_0561 0.43 -2.0 638,359 + Psyr_0561 0.43 -1.4 638,359 + Psyr_0561 0.43 -1.6 638,360 - Psyr_0561 0.43 -2.1 638,360 - Psyr_0561 0.43 -0.2 638,360 - Psyr_0561 0.43 -1.1 638,360 - Psyr_0561 0.43 -0.7 638,360 - Psyr_0561 0.43 -1.4 638,499 + Psyr_0561 0.60 -0.4 638,594 + Psyr_0561 0.71 +0.8 638,594 + Psyr_0561 0.71 -0.9 638,594 + Psyr_0561 0.71 +0.3 638,594 + Psyr_0561 0.71 -1.1 638,594 + Psyr_0561 0.71 -1.5 638,594 + Psyr_0561 0.71 +0.1 638,595 - Psyr_0561 0.71 -0.5 638,595 - Psyr_0561 0.71 -1.8 638,595 - Psyr_0561 0.71 -0.2 638,595 - Psyr_0561 0.71 +1.2 638,595 - Psyr_0561 0.71 +0.3 638,595 - Psyr_0561 0.71 +0.2 638,595 - Psyr_0561 0.71 +0.3 638,595 - Psyr_0561 0.71 -0.8 638,786 - -0.6 638,839 + -0.6 638,854 - -1.3 639,061 + Psyr_0562 0.21 +0.1 639,062 - Psyr_0562 0.21 -0.4 639,374 - Psyr_0562 0.50 -1.3 639,598 + Psyr_0562 0.72 +0.4 639,598 + Psyr_0562 0.72 -0.3 639,598 + Psyr_0562 0.72 -1.2 639,598 + Psyr_0562 0.72 -1.0 639,598 + Psyr_0562 0.72 -1.4 639,599 - Psyr_0562 0.72 -0.4 639,599 - Psyr_0562 0.72 -0.1 639,814 + +0.5 639,814 + -0.0
Or see this region's nucleotide sequence