Experiment: library passed through aphids in food, 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0404 and Psyr_0405 overlap by 4 nucleotides Psyr_0405 and Psyr_0406 overlap by 4 nucleotides Psyr_0406 and Psyr_0407 overlap by 4 nucleotides
Psyr_0404: Psyr_0404 - Fimbrial assembly, at 434,688 to 435,263
_0404
Psyr_0405: Psyr_0405 - Pilus assembly protein, PilO, at 435,260 to 435,883
_0405
Psyr_0406: Psyr_0406 - Pilus assembly protein, PilQ, at 435,880 to 436,407
_0406
Psyr_0407: Psyr_0407 - type II and III secretion system protein:NolW-like protein:NolW-like protein, at 436,404 to 438,560
_0407
Position (kb) 435
436
437 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 435.027 kb on + strand, within Psyr_0404 at 435.028 kb on - strand, within Psyr_0404 at 435.028 kb on - strand, within Psyr_0404 at 435.028 kb on - strand, within Psyr_0404 at 435.030 kb on + strand, within Psyr_0404 at 435.214 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.266 kb on - strand at 435.266 kb on - strand at 435.266 kb on - strand at 435.266 kb on - strand at 435.266 kb on - strand at 435.266 kb on - strand at 435.266 kb on - strand at 435.266 kb on - strand at 435.398 kb on + strand, within Psyr_0405 at 435.398 kb on + strand, within Psyr_0405 at 435.398 kb on + strand, within Psyr_0405 at 435.399 kb on - strand, within Psyr_0405 at 435.512 kb on + strand, within Psyr_0405 at 435.513 kb on - strand, within Psyr_0405 at 435.680 kb on + strand, within Psyr_0405 at 435.680 kb on + strand, within Psyr_0405 at 435.680 kb on + strand, within Psyr_0405 at 435.680 kb on + strand, within Psyr_0405 at 435.681 kb on - strand, within Psyr_0405 at 435.852 kb on - strand at 435.858 kb on - strand at 435.858 kb on - strand at 436.048 kb on + strand, within Psyr_0406 at 436.048 kb on + strand, within Psyr_0406 at 436.048 kb on + strand, within Psyr_0406 at 436.048 kb on + strand, within Psyr_0406 at 436.049 kb on - strand, within Psyr_0406 at 436.049 kb on - strand, within Psyr_0406 at 436.049 kb on - strand, within Psyr_0406 at 436.276 kb on + strand, within Psyr_0406 at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.425 kb on - strand at 436.425 kb on - strand at 436.425 kb on - strand at 436.425 kb on - strand at 436.425 kb on - strand at 436.425 kb on - strand at 436.425 kb on - strand at 436.425 kb on - strand at 436.425 kb on - strand at 436.425 kb on - strand at 436.489 kb on + strand at 436.618 kb on - strand at 436.665 kb on - strand, within Psyr_0407 at 436.729 kb on + strand, within Psyr_0407 at 437.202 kb on + strand, within Psyr_0407 at 437.295 kb on + strand, within Psyr_0407 at 437.295 kb on + strand, within Psyr_0407 at 437.296 kb on - strand, within Psyr_0407 at 437.296 kb on - strand, within Psyr_0407 at 437.397 kb on - strand, within Psyr_0407
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 24 hours remove 435,027 + Psyr_0404 0.59 -0.5 435,028 - Psyr_0404 0.59 -0.2 435,028 - Psyr_0404 0.59 -0.6 435,028 - Psyr_0404 0.59 -0.8 435,030 + Psyr_0404 0.59 -0.1 435,214 + -0.6 435,265 + -0.1 435,265 + -1.0 435,265 + -0.2 435,265 + -0.1 435,265 + -1.3 435,265 + -0.6 435,265 + +0.0 435,265 + +1.2 435,265 + -1.1 435,265 + +0.2 435,265 + -0.2 435,265 + +0.3 435,265 + -1.3 435,265 + -0.9 435,265 + -0.8 435,265 + -1.2 435,265 + +0.5 435,266 - -0.8 435,266 - +1.4 435,266 - +0.6 435,266 - +0.6 435,266 - -0.1 435,266 - +0.0 435,266 - -1.2 435,266 - +1.2 435,398 + Psyr_0405 0.22 -0.9 435,398 + Psyr_0405 0.22 -0.5 435,398 + Psyr_0405 0.22 +0.8 435,399 - Psyr_0405 0.22 +0.3 435,512 + Psyr_0405 0.40 +0.4 435,513 - Psyr_0405 0.41 -0.5 435,680 + Psyr_0405 0.67 -0.8 435,680 + Psyr_0405 0.67 -0.7 435,680 + Psyr_0405 0.67 -0.1 435,680 + Psyr_0405 0.67 +0.1 435,681 - Psyr_0405 0.67 -0.2 435,852 - +0.4 435,858 - -0.4 435,858 - +0.3 436,048 + Psyr_0406 0.32 -0.4 436,048 + Psyr_0406 0.32 -3.3 436,048 + Psyr_0406 0.32 -0.3 436,048 + Psyr_0406 0.32 -1.4 436,049 - Psyr_0406 0.32 -0.4 436,049 - Psyr_0406 0.32 +0.5 436,049 - Psyr_0406 0.32 -1.3 436,276 + Psyr_0406 0.75 -0.1 436,424 + -0.1 436,424 + -0.3 436,424 + +0.1 436,424 + +0.4 436,424 + -0.4 436,424 + +0.7 436,424 + -1.0 436,424 + -0.2 436,424 + +0.4 436,424 + +0.1 436,424 + -0.1 436,424 + -0.7 436,424 + -0.2 436,424 + -0.6 436,424 + -0.4 436,425 - -0.5 436,425 - +1.2 436,425 - -0.5 436,425 - -0.1 436,425 - -0.3 436,425 - +0.0 436,425 - -0.5 436,425 - -0.8 436,425 - -1.5 436,425 - -0.8 436,489 + -0.0 436,618 - -0.8 436,665 - Psyr_0407 0.12 -0.7 436,729 + Psyr_0407 0.15 -0.2 437,202 + Psyr_0407 0.37 -0.8 437,295 + Psyr_0407 0.41 -0.5 437,295 + Psyr_0407 0.41 +0.5 437,296 - Psyr_0407 0.41 -0.5 437,296 - Psyr_0407 0.41 -1.5 437,397 - Psyr_0407 0.46 -0.0
Or see this region's nucleotide sequence