Experiment: library passed through aphids in food, 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0289 and Psyr_0290 are separated by 122 nucleotides Psyr_0290 and Psyr_0291 are separated by 186 nucleotides Psyr_0291 and Psyr_0292 are separated by 231 nucleotides
Psyr_0289: Psyr_0289 - Radical SAM, at 307,483 to 308,541
_0289
Psyr_0290: Psyr_0290 - ThiJ/PfpI, at 308,664 to 309,332
_0290
Psyr_0291: Psyr_0291 - DedA, at 309,519 to 310,181
_0291
Psyr_0292: Psyr_0292 - porphobilinogen synthase, at 310,413 to 311,423
_0292
Position (kb) 309
310
311 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 308.549 kb on + strand at 308.549 kb on + strand at 308.550 kb on - strand at 308.557 kb on + strand at 308.570 kb on + strand at 308.575 kb on + strand at 308.582 kb on + strand at 308.582 kb on + strand at 308.583 kb on - strand at 308.755 kb on + strand, within Psyr_0290 at 308.755 kb on + strand, within Psyr_0290 at 308.755 kb on + strand, within Psyr_0290 at 308.755 kb on + strand, within Psyr_0290 at 308.755 kb on + strand, within Psyr_0290 at 308.755 kb on + strand, within Psyr_0290 at 308.755 kb on + strand, within Psyr_0290 at 308.755 kb on + strand, within Psyr_0290 at 308.756 kb on - strand, within Psyr_0290 at 308.756 kb on - strand, within Psyr_0290 at 308.756 kb on - strand, within Psyr_0290 at 308.756 kb on - strand, within Psyr_0290 at 308.756 kb on - strand, within Psyr_0290 at 308.756 kb on - strand, within Psyr_0290 at 308.756 kb on - strand, within Psyr_0290 at 308.756 kb on - strand, within Psyr_0290 at 308.896 kb on + strand, within Psyr_0290 at 308.896 kb on + strand, within Psyr_0290 at 308.896 kb on + strand, within Psyr_0290 at 308.897 kb on - strand, within Psyr_0290 at 309.290 kb on - strand at 309.312 kb on + strand at 309.312 kb on + strand at 309.313 kb on - strand at 309.374 kb on + strand at 309.375 kb on - strand at 309.521 kb on - strand at 309.709 kb on + strand, within Psyr_0291 at 309.710 kb on - strand, within Psyr_0291 at 309.758 kb on - strand, within Psyr_0291 at 309.817 kb on + strand, within Psyr_0291 at 309.817 kb on + strand, within Psyr_0291 at 310.007 kb on - strand, within Psyr_0291 at 310.007 kb on - strand, within Psyr_0291 at 310.007 kb on - strand, within Psyr_0291 at 310.027 kb on + strand, within Psyr_0291 at 310.027 kb on + strand, within Psyr_0291 at 310.027 kb on + strand, within Psyr_0291 at 310.028 kb on - strand, within Psyr_0291 at 310.028 kb on - strand, within Psyr_0291 at 310.044 kb on + strand, within Psyr_0291 at 310.073 kb on - strand, within Psyr_0291 at 310.073 kb on - strand, within Psyr_0291 at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.289 kb on - strand at 310.289 kb on - strand at 310.289 kb on - strand at 310.367 kb on + strand at 310.367 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 24 hours remove 308,549 + -0.2 308,549 + +0.7 308,550 - +0.0 308,557 + -0.3 308,570 + -0.3 308,575 + +0.8 308,582 + -0.3 308,582 + +0.8 308,583 - +0.1 308,755 + Psyr_0290 0.14 +0.1 308,755 + Psyr_0290 0.14 -1.4 308,755 + Psyr_0290 0.14 +0.2 308,755 + Psyr_0290 0.14 -0.6 308,755 + Psyr_0290 0.14 -0.1 308,755 + Psyr_0290 0.14 +0.7 308,755 + Psyr_0290 0.14 -0.1 308,755 + Psyr_0290 0.14 -0.9 308,756 - Psyr_0290 0.14 +0.9 308,756 - Psyr_0290 0.14 +0.6 308,756 - Psyr_0290 0.14 +1.2 308,756 - Psyr_0290 0.14 -0.2 308,756 - Psyr_0290 0.14 +0.3 308,756 - Psyr_0290 0.14 -0.2 308,756 - Psyr_0290 0.14 +0.7 308,756 - Psyr_0290 0.14 +0.4 308,896 + Psyr_0290 0.35 -0.2 308,896 + Psyr_0290 0.35 -2.3 308,896 + Psyr_0290 0.35 -0.0 308,897 - Psyr_0290 0.35 -0.3 309,290 - -0.2 309,312 + -0.5 309,312 + +0.4 309,313 - +0.2 309,374 + +0.2 309,375 - -0.6 309,521 - +0.3 309,709 + Psyr_0291 0.29 -0.9 309,710 - Psyr_0291 0.29 -1.0 309,758 - Psyr_0291 0.36 -0.6 309,817 + Psyr_0291 0.45 -0.0 309,817 + Psyr_0291 0.45 -1.5 310,007 - Psyr_0291 0.74 -1.1 310,007 - Psyr_0291 0.74 +0.3 310,007 - Psyr_0291 0.74 -1.0 310,027 + Psyr_0291 0.77 -3.0 310,027 + Psyr_0291 0.77 -0.8 310,027 + Psyr_0291 0.77 -0.9 310,028 - Psyr_0291 0.77 -1.2 310,028 - Psyr_0291 0.77 -0.8 310,044 + Psyr_0291 0.79 +0.0 310,073 - Psyr_0291 0.84 -0.8 310,073 - Psyr_0291 0.84 -0.2 310,246 + -0.9 310,246 + -0.4 310,246 + -0.2 310,246 + -1.3 310,246 + -0.7 310,246 + -1.2 310,246 + -0.9 310,246 + -0.2 310,246 + -1.4 310,246 + +0.2 310,246 + +0.5 310,246 + -0.5 310,246 + -0.7 310,246 + -2.3 310,247 - -0.2 310,247 - -0.2 310,247 - -0.0 310,247 - -0.7 310,247 - +0.1 310,247 - -0.5 310,247 - -0.4 310,247 - -1.0 310,247 - +0.4 310,247 - -1.2 310,247 - -0.4 310,247 - +0.4 310,247 - +0.2 310,289 - +0.2 310,289 - -1.9 310,289 - -0.1 310,367 + -0.5 310,367 + -0.1 310,369 + +0.5 310,369 + -0.3 310,369 + +0.1 310,369 + +0.4 310,369 + +0.4 310,369 + +0.7 310,369 + -0.0
Or see this region's nucleotide sequence