Experiment: library passed through aphids in food, 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0254 and Psyr_0255 are separated by 314 nucleotides Psyr_0255 and Psyr_0256 are separated by 226 nucleotides Psyr_0256 and Psyr_0257 overlap by 1 nucleotides
Psyr_0254: Psyr_0254 - N-acetylglutamate synthase, at 267,685 to 268,983
_0254
Psyr_0255: Psyr_0255 - glutamate-cysteine ligase, at 269,298 to 270,905
_0255
Psyr_0256: Psyr_0256 - Phenylacetic acid degradation-related protein, at 271,132 to 271,515
_0256
Psyr_0257: Psyr_0257 - RNA binding S1, at 271,515 to 273,833
_0257
Position (kb) 269
270
271 Strain fitness (log2 ratio) -2
-1
0
1
2 at 268.979 kb on + strand at 268.981 kb on + strand at 268.981 kb on + strand at 268.981 kb on + strand at 268.981 kb on + strand at 268.981 kb on + strand at 268.981 kb on + strand at 268.981 kb on + strand at 268.981 kb on + strand at 268.981 kb on + strand at 268.981 kb on + strand at 268.981 kb on + strand at 268.981 kb on + strand at 268.981 kb on + strand at 268.981 kb on + strand at 268.982 kb on - strand at 268.982 kb on - strand at 268.982 kb on - strand at 268.982 kb on - strand at 268.982 kb on - strand at 268.982 kb on - strand at 268.982 kb on - strand at 268.982 kb on - strand at 268.982 kb on - strand at 268.998 kb on + strand at 269.000 kb on + strand at 269.000 kb on + strand at 269.000 kb on + strand at 269.000 kb on + strand at 269.000 kb on + strand at 269.000 kb on + strand at 269.000 kb on + strand at 269.000 kb on + strand at 269.000 kb on + strand at 269.001 kb on - strand at 269.001 kb on - strand at 269.001 kb on - strand at 269.130 kb on - strand at 269.130 kb on - strand at 269.243 kb on + strand at 269.287 kb on + strand at 269.287 kb on + strand at 269.287 kb on + strand at 269.287 kb on + strand at 269.287 kb on + strand at 269.287 kb on + strand at 269.288 kb on - strand at 269.288 kb on - strand at 269.288 kb on - strand at 269.288 kb on - strand at 269.288 kb on - strand at 269.288 kb on - strand at 269.288 kb on - strand at 269.329 kb on + strand at 270.128 kb on - strand, within Psyr_0255 at 270.205 kb on + strand, within Psyr_0255 at 270.205 kb on + strand, within Psyr_0255 at 270.296 kb on - strand, within Psyr_0255 at 270.296 kb on - strand, within Psyr_0255 at 270.500 kb on - strand, within Psyr_0255 at 270.896 kb on - strand at 270.896 kb on - strand at 270.944 kb on + strand at 270.944 kb on + strand at 270.971 kb on - strand at 271.022 kb on - strand at 271.065 kb on - strand at 271.088 kb on + strand at 271.133 kb on + strand at 271.133 kb on + strand at 271.134 kb on - strand at 271.134 kb on - strand at 271.134 kb on - strand at 271.274 kb on + strand, within Psyr_0256 at 271.274 kb on + strand, within Psyr_0256 at 271.274 kb on + strand, within Psyr_0256 at 271.274 kb on + strand, within Psyr_0256 at 271.275 kb on - strand, within Psyr_0256 at 271.275 kb on - strand, within Psyr_0256 at 271.275 kb on - strand, within Psyr_0256 at 271.275 kb on - strand, within Psyr_0256 at 271.275 kb on - strand, within Psyr_0256 at 271.275 kb on - strand, within Psyr_0256 at 271.275 kb on - strand, within Psyr_0256 at 271.275 kb on - strand, within Psyr_0256 at 271.275 kb on - strand, within Psyr_0256 at 271.275 kb on - strand, within Psyr_0256
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 24 hours remove 268,979 + +0.3 268,981 + -0.2 268,981 + -0.0 268,981 + -0.3 268,981 + +0.5 268,981 + -1.0 268,981 + +0.9 268,981 + +0.4 268,981 + +0.9 268,981 + -0.8 268,981 + +1.1 268,981 + +0.3 268,981 + -0.3 268,981 + -0.3 268,981 + +0.9 268,982 - -1.7 268,982 - -0.1 268,982 - +0.3 268,982 - -0.6 268,982 - +0.6 268,982 - +0.6 268,982 - -0.1 268,982 - -0.6 268,982 - +0.7 268,998 + -0.6 269,000 + +0.4 269,000 + -0.2 269,000 + +0.8 269,000 + -0.4 269,000 + -0.7 269,000 + +0.0 269,000 + +0.4 269,000 + -0.0 269,000 + +0.0 269,001 - -0.2 269,001 - +0.1 269,001 - +0.1 269,130 - +0.1 269,130 - -1.2 269,243 + -1.6 269,287 + -1.2 269,287 + -0.1 269,287 + +0.6 269,287 + -1.6 269,287 + -0.3 269,287 + +0.5 269,288 - +0.8 269,288 - +0.3 269,288 - -0.2 269,288 - +0.2 269,288 - -0.3 269,288 - -0.5 269,288 - -1.0 269,329 + +0.8 270,128 - Psyr_0255 0.52 -1.0 270,205 + Psyr_0255 0.56 -1.6 270,205 + Psyr_0255 0.56 +0.4 270,296 - Psyr_0255 0.62 -2.0 270,296 - Psyr_0255 0.62 -2.5 270,500 - Psyr_0255 0.75 -0.7 270,896 - +0.8 270,896 - -0.1 270,944 + -1.0 270,944 + +0.0 270,971 - +0.0 271,022 - -0.2 271,065 - -0.2 271,088 + +0.7 271,133 + +0.6 271,133 + -0.4 271,134 - -0.4 271,134 - -0.6 271,134 - -1.3 271,274 + Psyr_0256 0.37 -1.1 271,274 + Psyr_0256 0.37 -0.4 271,274 + Psyr_0256 0.37 -0.2 271,274 + Psyr_0256 0.37 +0.0 271,275 - Psyr_0256 0.37 -0.2 271,275 - Psyr_0256 0.37 +0.6 271,275 - Psyr_0256 0.37 +2.0 271,275 - Psyr_0256 0.37 +1.4 271,275 - Psyr_0256 0.37 -0.5 271,275 - Psyr_0256 0.37 +0.8 271,275 - Psyr_0256 0.37 -0.1 271,275 - Psyr_0256 0.37 -0.6 271,275 - Psyr_0256 0.37 +0.1 271,275 - Psyr_0256 0.37 +0.3
Or see this region's nucleotide sequence