Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4825 and Pf6N2E2_4826 overlap by 4 nucleotides Pf6N2E2_4826 and Pf6N2E2_4827 are separated by 3 nucleotides Pf6N2E2_4827 and Pf6N2E2_4828 overlap by 4 nucleotides Pf6N2E2_4828 and Pf6N2E2_4829 are separated by 238 nucleotides
Pf6N2E2_4825 - Anthranilate phosphoribosyltransferase (EC 2.4.2.18), at 5,579,815 to 5,580,864
_4825
Pf6N2E2_4826 - Indole-3-glycerol phosphate synthase (EC 4.1.1.48), at 5,580,861 to 5,581,697
_4826
Pf6N2E2_4827 - Lipoate-protein ligase A, at 5,581,701 to 5,582,402
_4827
Pf6N2E2_4828 - Vfr transcriptional regulator / Cyclic AMP receptor protein, at 5,582,399 to 5,583,043
_4828
Pf6N2E2_4829 - OsmC/Ohr family protein, at 5,583,282 to 5,583,704
_4829
Position (kb)
5581
5582
5583 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5580.728 kb on - strand, within Pf6N2E2_4825 at 5580.728 kb on - strand, within Pf6N2E2_4825 at 5580.831 kb on + strand at 5580.831 kb on + strand at 5580.831 kb on + strand at 5580.831 kb on + strand at 5580.831 kb on + strand at 5580.832 kb on - strand at 5580.832 kb on - strand at 5580.839 kb on + strand at 5580.839 kb on + strand at 5580.840 kb on - strand at 5580.966 kb on - strand, within Pf6N2E2_4826 at 5581.113 kb on + strand, within Pf6N2E2_4826 at 5581.130 kb on + strand, within Pf6N2E2_4826 at 5581.130 kb on + strand, within Pf6N2E2_4826 at 5581.130 kb on + strand, within Pf6N2E2_4826 at 5581.182 kb on + strand, within Pf6N2E2_4826 at 5581.584 kb on + strand, within Pf6N2E2_4826 at 5581.584 kb on + strand, within Pf6N2E2_4826 at 5581.584 kb on + strand, within Pf6N2E2_4826 at 5581.584 kb on + strand, within Pf6N2E2_4826 at 5581.584 kb on + strand, within Pf6N2E2_4826 at 5581.584 kb on + strand, within Pf6N2E2_4826 at 5581.584 kb on + strand, within Pf6N2E2_4826 at 5581.584 kb on + strand, within Pf6N2E2_4826 at 5581.584 kb on + strand, within Pf6N2E2_4826 at 5581.867 kb on - strand, within Pf6N2E2_4827 at 5581.879 kb on + strand, within Pf6N2E2_4827 at 5581.959 kb on - strand, within Pf6N2E2_4827 at 5581.971 kb on + strand, within Pf6N2E2_4827 at 5581.971 kb on + strand, within Pf6N2E2_4827 at 5581.972 kb on - strand, within Pf6N2E2_4827 at 5581.972 kb on - strand, within Pf6N2E2_4827 at 5581.972 kb on - strand, within Pf6N2E2_4827 at 5582.006 kb on + strand, within Pf6N2E2_4827 at 5582.367 kb on + strand at 5582.650 kb on + strand, within Pf6N2E2_4828 at 5582.650 kb on + strand, within Pf6N2E2_4828 at 5582.651 kb on - strand, within Pf6N2E2_4828 at 5582.651 kb on - strand, within Pf6N2E2_4828 at 5582.651 kb on - strand, within Pf6N2E2_4828 at 5582.685 kb on - strand, within Pf6N2E2_4828 at 5582.685 kb on - strand, within Pf6N2E2_4828 at 5582.685 kb on - strand, within Pf6N2E2_4828 at 5582.685 kb on - strand, within Pf6N2E2_4828 at 5582.688 kb on + strand, within Pf6N2E2_4828 at 5582.688 kb on + strand, within Pf6N2E2_4828 at 5582.689 kb on - strand, within Pf6N2E2_4828 at 5582.689 kb on - strand, within Pf6N2E2_4828 at 5582.733 kb on + strand, within Pf6N2E2_4828 at 5582.813 kb on + strand, within Pf6N2E2_4828 at 5582.850 kb on + strand, within Pf6N2E2_4828 at 5582.850 kb on + strand, within Pf6N2E2_4828 at 5582.851 kb on - strand, within Pf6N2E2_4828 at 5582.851 kb on - strand, within Pf6N2E2_4828 at 5582.851 kb on - strand, within Pf6N2E2_4828 at 5582.885 kb on + strand, within Pf6N2E2_4828 at 5582.885 kb on + strand, within Pf6N2E2_4828 at 5582.885 kb on + strand, within Pf6N2E2_4828 at 5582.885 kb on + strand, within Pf6N2E2_4828 at 5582.885 kb on + strand, within Pf6N2E2_4828 at 5582.885 kb on + strand, within Pf6N2E2_4828 at 5582.885 kb on + strand, within Pf6N2E2_4828 at 5582.885 kb on + strand, within Pf6N2E2_4828 at 5582.886 kb on - strand, within Pf6N2E2_4828 at 5582.886 kb on - strand, within Pf6N2E2_4828 at 5582.886 kb on - strand, within Pf6N2E2_4828 at 5582.893 kb on + strand, within Pf6N2E2_4828 at 5583.031 kb on + strand at 5583.031 kb on + strand at 5583.049 kb on + strand at 5583.049 kb on + strand at 5583.049 kb on + strand at 5583.102 kb on + strand at 5583.139 kb on + strand at 5583.140 kb on - strand at 5583.150 kb on + strand at 5583.150 kb on + strand at 5583.166 kb on - strand at 5583.166 kb on - strand at 5583.182 kb on + strand at 5583.182 kb on + strand at 5583.183 kb on - strand at 5583.186 kb on + strand at 5583.186 kb on + strand at 5583.186 kb on + strand at 5583.186 kb on + strand at 5583.186 kb on + strand at 5583.186 kb on + strand at 5583.186 kb on + strand at 5583.187 kb on - strand at 5583.196 kb on + strand at 5583.196 kb on + strand at 5583.196 kb on + strand at 5583.197 kb on - strand at 5583.210 kb on + strand at 5583.211 kb on - strand at 5583.211 kb on - strand at 5583.244 kb on + strand at 5583.244 kb on + strand at 5583.244 kb on + strand at 5583.244 kb on + strand at 5583.244 kb on + strand at 5583.244 kb on + strand at 5583.263 kb on + strand at 5583.263 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 5,580,728 - Pf6N2E2_4825 0.87 -3.1 5,580,728 - Pf6N2E2_4825 0.87 -3.2 5,580,831 + -2.4 5,580,831 + -4.1 5,580,831 + -2.4 5,580,831 + -4.0 5,580,831 + -4.0 5,580,832 - -2.2 5,580,832 - -3.4 5,580,839 + -2.0 5,580,839 + -2.6 5,580,840 - -2.2 5,580,966 - Pf6N2E2_4826 0.13 -0.4 5,581,113 + Pf6N2E2_4826 0.30 -4.2 5,581,130 + Pf6N2E2_4826 0.32 -2.7 5,581,130 + Pf6N2E2_4826 0.32 -2.1 5,581,130 + Pf6N2E2_4826 0.32 -3.5 5,581,182 + Pf6N2E2_4826 0.38 -2.6 5,581,584 + Pf6N2E2_4826 0.86 -4.2 5,581,584 + Pf6N2E2_4826 0.86 -2.1 5,581,584 + Pf6N2E2_4826 0.86 -2.8 5,581,584 + Pf6N2E2_4826 0.86 -3.8 5,581,584 + Pf6N2E2_4826 0.86 -4.3 5,581,584 + Pf6N2E2_4826 0.86 -4.0 5,581,584 + Pf6N2E2_4826 0.86 -3.4 5,581,584 + Pf6N2E2_4826 0.86 -2.3 5,581,584 + Pf6N2E2_4826 0.86 -3.6 5,581,867 - Pf6N2E2_4827 0.24 -1.1 5,581,879 + Pf6N2E2_4827 0.25 -0.6 5,581,959 - Pf6N2E2_4827 0.37 -2.0 5,581,971 + Pf6N2E2_4827 0.38 -0.4 5,581,971 + Pf6N2E2_4827 0.38 -1.0 5,581,972 - Pf6N2E2_4827 0.39 -2.5 5,581,972 - Pf6N2E2_4827 0.39 -1.0 5,581,972 - Pf6N2E2_4827 0.39 -0.8 5,582,006 + Pf6N2E2_4827 0.43 -0.4 5,582,367 + -0.5 5,582,650 + Pf6N2E2_4828 0.39 +0.2 5,582,650 + Pf6N2E2_4828 0.39 +0.5 5,582,651 - Pf6N2E2_4828 0.39 -0.2 5,582,651 - Pf6N2E2_4828 0.39 +0.0 5,582,651 - Pf6N2E2_4828 0.39 -0.2 5,582,685 - Pf6N2E2_4828 0.44 -1.8 5,582,685 - Pf6N2E2_4828 0.44 +0.1 5,582,685 - Pf6N2E2_4828 0.44 +0.1 5,582,685 - Pf6N2E2_4828 0.44 +0.2 5,582,688 + Pf6N2E2_4828 0.45 -1.0 5,582,688 + Pf6N2E2_4828 0.45 -0.0 5,582,689 - Pf6N2E2_4828 0.45 -0.0 5,582,689 - Pf6N2E2_4828 0.45 -0.0 5,582,733 + Pf6N2E2_4828 0.52 -0.1 5,582,813 + Pf6N2E2_4828 0.64 -0.3 5,582,850 + Pf6N2E2_4828 0.70 +0.0 5,582,850 + Pf6N2E2_4828 0.70 +0.6 5,582,851 - Pf6N2E2_4828 0.70 -0.1 5,582,851 - Pf6N2E2_4828 0.70 +0.2 5,582,851 - Pf6N2E2_4828 0.70 +1.0 5,582,885 + Pf6N2E2_4828 0.75 +0.3 5,582,885 + Pf6N2E2_4828 0.75 +0.6 5,582,885 + Pf6N2E2_4828 0.75 +0.5 5,582,885 + Pf6N2E2_4828 0.75 -0.9 5,582,885 + Pf6N2E2_4828 0.75 +0.4 5,582,885 + Pf6N2E2_4828 0.75 +0.3 5,582,885 + Pf6N2E2_4828 0.75 +3.2 5,582,885 + Pf6N2E2_4828 0.75 -0.0 5,582,886 - Pf6N2E2_4828 0.76 -0.8 5,582,886 - Pf6N2E2_4828 0.76 +0.8 5,582,886 - Pf6N2E2_4828 0.76 -0.3 5,582,893 + Pf6N2E2_4828 0.77 +0.1 5,583,031 + +0.4 5,583,031 + -0.3 5,583,049 + -0.3 5,583,049 + -1.4 5,583,049 + +0.4 5,583,102 + -2.4 5,583,139 + -3.8 5,583,140 - -0.4 5,583,150 + +0.5 5,583,150 + -0.1 5,583,166 - +0.2 5,583,166 - +0.6 5,583,182 + +0.4 5,583,182 + -0.3 5,583,183 - -0.2 5,583,186 + +0.6 5,583,186 + +0.1 5,583,186 + +0.2 5,583,186 + +0.6 5,583,186 + +0.8 5,583,186 + -0.3 5,583,186 + -0.0 5,583,187 - +0.9 5,583,196 + +0.3 5,583,196 + +0.2 5,583,196 + +0.1 5,583,197 - -1.1 5,583,210 + +1.0 5,583,211 - -1.4 5,583,211 - +0.3 5,583,244 + -1.3 5,583,244 + +0.2 5,583,244 + -1.2 5,583,244 + +0.2 5,583,244 + -0.5 5,583,244 + -0.3 5,583,263 + -0.1 5,583,263 + -0.4
Or see this region's nucleotide sequence