Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3521 and Pf6N2E2_3522 are separated by 15 nucleotides Pf6N2E2_3522 and Pf6N2E2_3523 are separated by 274 nucleotides
Pf6N2E2_3521 - Sensor histidine kinase/response regulator, at 4,105,789 to 4,108,578
_3521
Pf6N2E2_3522 - hypothetical protein, at 4,108,594 to 4,108,989
_3522
Pf6N2E2_3523 - hypothetical protein, at 4,109,264 to 4,109,983
_3523
Position (kb)
4108
4109 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4107.630 kb on + strand, within Pf6N2E2_3521 at 4107.630 kb on + strand, within Pf6N2E2_3521 at 4107.631 kb on - strand, within Pf6N2E2_3521 at 4107.752 kb on + strand, within Pf6N2E2_3521 at 4107.853 kb on + strand, within Pf6N2E2_3521 at 4108.050 kb on + strand, within Pf6N2E2_3521 at 4108.112 kb on + strand, within Pf6N2E2_3521 at 4108.112 kb on + strand, within Pf6N2E2_3521 at 4108.112 kb on + strand, within Pf6N2E2_3521 at 4108.112 kb on + strand, within Pf6N2E2_3521 at 4108.113 kb on - strand, within Pf6N2E2_3521 at 4108.113 kb on - strand, within Pf6N2E2_3521 at 4108.113 kb on - strand, within Pf6N2E2_3521 at 4108.390 kb on - strand at 4108.614 kb on + strand at 4108.614 kb on + strand at 4108.614 kb on + strand at 4108.615 kb on - strand at 4108.618 kb on - strand at 4108.670 kb on + strand, within Pf6N2E2_3522 at 4108.671 kb on - strand, within Pf6N2E2_3522 at 4108.671 kb on - strand, within Pf6N2E2_3522 at 4108.671 kb on - strand, within Pf6N2E2_3522 at 4108.671 kb on - strand, within Pf6N2E2_3522 at 4108.764 kb on + strand, within Pf6N2E2_3522 at 4108.765 kb on - strand, within Pf6N2E2_3522 at 4108.765 kb on - strand, within Pf6N2E2_3522 at 4108.765 kb on - strand, within Pf6N2E2_3522 at 4108.765 kb on - strand, within Pf6N2E2_3522 at 4108.787 kb on + strand, within Pf6N2E2_3522 at 4108.787 kb on + strand, within Pf6N2E2_3522 at 4108.788 kb on - strand, within Pf6N2E2_3522 at 4108.802 kb on - strand, within Pf6N2E2_3522 at 4108.802 kb on - strand, within Pf6N2E2_3522 at 4108.802 kb on - strand, within Pf6N2E2_3522 at 4109.045 kb on + strand at 4109.046 kb on - strand at 4109.063 kb on + strand at 4109.064 kb on - strand at 4109.064 kb on - strand at 4109.064 kb on - strand at 4109.064 kb on - strand at 4109.067 kb on - strand at 4109.126 kb on + strand at 4109.127 kb on - strand at 4109.127 kb on - strand at 4109.127 kb on - strand at 4109.127 kb on - strand at 4109.303 kb on - strand at 4109.303 kb on - strand at 4109.327 kb on - strand at 4109.327 kb on - strand at 4109.342 kb on + strand, within Pf6N2E2_3523 at 4109.368 kb on - strand, within Pf6N2E2_3523 at 4109.382 kb on - strand, within Pf6N2E2_3523 at 4109.461 kb on + strand, within Pf6N2E2_3523 at 4109.481 kb on + strand, within Pf6N2E2_3523 at 4109.481 kb on + strand, within Pf6N2E2_3523 at 4109.481 kb on + strand, within Pf6N2E2_3523 at 4109.481 kb on + strand, within Pf6N2E2_3523 at 4109.482 kb on - strand, within Pf6N2E2_3523 at 4109.482 kb on - strand, within Pf6N2E2_3523 at 4109.482 kb on - strand, within Pf6N2E2_3523 at 4109.482 kb on - strand, within Pf6N2E2_3523 at 4109.482 kb on - strand, within Pf6N2E2_3523 at 4109.686 kb on + strand, within Pf6N2E2_3523 at 4109.686 kb on + strand, within Pf6N2E2_3523 at 4109.687 kb on - strand, within Pf6N2E2_3523 at 4109.687 kb on - strand, within Pf6N2E2_3523 at 4109.687 kb on - strand, within Pf6N2E2_3523 at 4109.697 kb on + strand, within Pf6N2E2_3523 at 4109.697 kb on + strand, within Pf6N2E2_3523 at 4109.697 kb on + strand, within Pf6N2E2_3523 at 4109.697 kb on + strand, within Pf6N2E2_3523 at 4109.697 kb on + strand, within Pf6N2E2_3523 at 4109.698 kb on - strand, within Pf6N2E2_3523 at 4109.698 kb on - strand, within Pf6N2E2_3523 at 4109.698 kb on - strand, within Pf6N2E2_3523 at 4109.814 kb on + strand, within Pf6N2E2_3523 at 4109.823 kb on + strand, within Pf6N2E2_3523 at 4109.824 kb on - strand, within Pf6N2E2_3523 at 4109.828 kb on + strand, within Pf6N2E2_3523 at 4109.829 kb on - strand, within Pf6N2E2_3523 at 4109.829 kb on - strand, within Pf6N2E2_3523 at 4109.844 kb on + strand, within Pf6N2E2_3523 at 4109.956 kb on + strand at 4109.977 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 4,107,630 + Pf6N2E2_3521 0.66 -0.2 4,107,630 + Pf6N2E2_3521 0.66 +0.3 4,107,631 - Pf6N2E2_3521 0.66 +2.0 4,107,752 + Pf6N2E2_3521 0.70 +1.5 4,107,853 + Pf6N2E2_3521 0.74 +1.6 4,108,050 + Pf6N2E2_3521 0.81 +1.1 4,108,112 + Pf6N2E2_3521 0.83 -2.1 4,108,112 + Pf6N2E2_3521 0.83 -1.1 4,108,112 + Pf6N2E2_3521 0.83 +1.3 4,108,112 + Pf6N2E2_3521 0.83 +0.5 4,108,113 - Pf6N2E2_3521 0.83 -0.3 4,108,113 - Pf6N2E2_3521 0.83 +0.3 4,108,113 - Pf6N2E2_3521 0.83 +0.1 4,108,390 - +1.1 4,108,614 + +0.1 4,108,614 + +0.6 4,108,614 + -0.3 4,108,615 - +0.9 4,108,618 - +0.4 4,108,670 + Pf6N2E2_3522 0.19 -1.4 4,108,671 - Pf6N2E2_3522 0.19 +0.2 4,108,671 - Pf6N2E2_3522 0.19 -0.8 4,108,671 - Pf6N2E2_3522 0.19 +1.2 4,108,671 - Pf6N2E2_3522 0.19 +1.1 4,108,764 + Pf6N2E2_3522 0.43 +0.1 4,108,765 - Pf6N2E2_3522 0.43 +0.4 4,108,765 - Pf6N2E2_3522 0.43 +0.9 4,108,765 - Pf6N2E2_3522 0.43 +0.2 4,108,765 - Pf6N2E2_3522 0.43 +1.6 4,108,787 + Pf6N2E2_3522 0.49 +0.8 4,108,787 + Pf6N2E2_3522 0.49 +1.1 4,108,788 - Pf6N2E2_3522 0.49 +1.6 4,108,802 - Pf6N2E2_3522 0.53 +0.2 4,108,802 - Pf6N2E2_3522 0.53 +0.3 4,108,802 - Pf6N2E2_3522 0.53 +0.6 4,109,045 + +0.3 4,109,046 - +0.6 4,109,063 + +0.2 4,109,064 - -0.1 4,109,064 - -1.6 4,109,064 - +0.2 4,109,064 - +0.9 4,109,067 - +1.6 4,109,126 + +0.3 4,109,127 - +0.8 4,109,127 - -1.9 4,109,127 - -0.6 4,109,127 - +0.3 4,109,303 - +0.3 4,109,303 - +0.3 4,109,327 - +1.1 4,109,327 - +1.7 4,109,342 + Pf6N2E2_3523 0.11 -1.2 4,109,368 - Pf6N2E2_3523 0.14 -0.6 4,109,382 - Pf6N2E2_3523 0.16 +0.3 4,109,461 + Pf6N2E2_3523 0.27 -0.3 4,109,481 + Pf6N2E2_3523 0.30 +0.8 4,109,481 + Pf6N2E2_3523 0.30 +0.1 4,109,481 + Pf6N2E2_3523 0.30 +0.5 4,109,481 + Pf6N2E2_3523 0.30 -1.3 4,109,482 - Pf6N2E2_3523 0.30 +0.2 4,109,482 - Pf6N2E2_3523 0.30 -0.4 4,109,482 - Pf6N2E2_3523 0.30 -0.2 4,109,482 - Pf6N2E2_3523 0.30 +0.9 4,109,482 - Pf6N2E2_3523 0.30 +0.2 4,109,686 + Pf6N2E2_3523 0.59 -0.5 4,109,686 + Pf6N2E2_3523 0.59 -0.3 4,109,687 - Pf6N2E2_3523 0.59 +0.2 4,109,687 - Pf6N2E2_3523 0.59 -0.1 4,109,687 - Pf6N2E2_3523 0.59 +0.2 4,109,697 + Pf6N2E2_3523 0.60 -0.3 4,109,697 + Pf6N2E2_3523 0.60 +0.4 4,109,697 + Pf6N2E2_3523 0.60 -0.2 4,109,697 + Pf6N2E2_3523 0.60 +0.4 4,109,697 + Pf6N2E2_3523 0.60 -0.5 4,109,698 - Pf6N2E2_3523 0.60 +0.8 4,109,698 - Pf6N2E2_3523 0.60 -0.0 4,109,698 - Pf6N2E2_3523 0.60 +1.3 4,109,814 + Pf6N2E2_3523 0.76 +0.4 4,109,823 + Pf6N2E2_3523 0.78 -2.0 4,109,824 - Pf6N2E2_3523 0.78 -0.4 4,109,828 + Pf6N2E2_3523 0.78 +0.1 4,109,829 - Pf6N2E2_3523 0.78 +1.5 4,109,829 - Pf6N2E2_3523 0.78 +1.0 4,109,844 + Pf6N2E2_3523 0.81 +0.8 4,109,956 + -0.9 4,109,977 + +0.5
Or see this region's nucleotide sequence