Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1316 and Pf6N2E2_1317 are separated by 66 nucleotides Pf6N2E2_1317 and Pf6N2E2_1318 are separated by 311 nucleotides
Pf6N2E2_1316 - P-hydroxyphenylacetate hydroxylase C1:reductase component, at 1,549,715 to 1,550,656
_1316
Pf6N2E2_1317 - P-hydroxyphenylacetate hydroxylase C2:oxygenase component, at 1,550,723 to 1,551,892
_1317
Pf6N2E2_1318 - FIG00553873: hypothetical protein, at 1,552,204 to 1,553,502
_1318
Position (kb)
1550
1551
1552 Strain fitness (log2 ratio)
-2
-1
0
1 at 1549.859 kb on + strand, within Pf6N2E2_1316 at 1550.046 kb on + strand, within Pf6N2E2_1316 at 1550.046 kb on + strand, within Pf6N2E2_1316 at 1550.047 kb on - strand, within Pf6N2E2_1316 at 1550.047 kb on - strand, within Pf6N2E2_1316 at 1550.059 kb on - strand, within Pf6N2E2_1316 at 1550.059 kb on - strand, within Pf6N2E2_1316 at 1550.085 kb on + strand, within Pf6N2E2_1316 at 1550.085 kb on + strand, within Pf6N2E2_1316 at 1550.599 kb on + strand at 1550.600 kb on - strand at 1550.693 kb on + strand at 1550.693 kb on + strand at 1550.701 kb on + strand at 1550.707 kb on + strand at 1550.790 kb on + strand at 1550.799 kb on + strand at 1550.800 kb on - strand at 1550.925 kb on + strand, within Pf6N2E2_1317 at 1550.925 kb on + strand, within Pf6N2E2_1317 at 1550.926 kb on - strand, within Pf6N2E2_1317 at 1550.988 kb on + strand, within Pf6N2E2_1317 at 1551.199 kb on - strand, within Pf6N2E2_1317 at 1551.199 kb on - strand, within Pf6N2E2_1317 at 1551.411 kb on + strand, within Pf6N2E2_1317 at 1551.735 kb on + strand, within Pf6N2E2_1317 at 1551.906 kb on + strand at 1551.907 kb on - strand at 1552.131 kb on - strand at 1552.133 kb on + strand at 1552.228 kb on + strand at 1552.228 kb on + strand at 1552.229 kb on - strand at 1552.229 kb on - strand at 1552.229 kb on - strand at 1552.231 kb on + strand at 1552.249 kb on + strand at 1552.249 kb on + strand at 1552.428 kb on + strand, within Pf6N2E2_1318 at 1552.549 kb on + strand, within Pf6N2E2_1318 at 1552.585 kb on + strand, within Pf6N2E2_1318 at 1552.586 kb on - strand, within Pf6N2E2_1318 at 1552.653 kb on + strand, within Pf6N2E2_1318 at 1552.717 kb on + strand, within Pf6N2E2_1318
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 1,549,859 + Pf6N2E2_1316 0.15 +1.2 1,550,046 + Pf6N2E2_1316 0.35 +0.3 1,550,046 + Pf6N2E2_1316 0.35 +0.5 1,550,047 - Pf6N2E2_1316 0.35 +0.2 1,550,047 - Pf6N2E2_1316 0.35 -0.9 1,550,059 - Pf6N2E2_1316 0.37 +0.2 1,550,059 - Pf6N2E2_1316 0.37 -0.4 1,550,085 + Pf6N2E2_1316 0.39 +1.5 1,550,085 + Pf6N2E2_1316 0.39 +0.5 1,550,599 + +1.7 1,550,600 - +0.3 1,550,693 + -0.1 1,550,693 + -1.6 1,550,701 + +1.4 1,550,707 + +0.1 1,550,790 + +0.4 1,550,799 + -0.9 1,550,800 - +0.5 1,550,925 + Pf6N2E2_1317 0.17 -0.6 1,550,925 + Pf6N2E2_1317 0.17 -1.1 1,550,926 - Pf6N2E2_1317 0.17 -0.8 1,550,988 + Pf6N2E2_1317 0.23 -1.2 1,551,199 - Pf6N2E2_1317 0.41 -0.5 1,551,199 - Pf6N2E2_1317 0.41 +1.0 1,551,411 + Pf6N2E2_1317 0.59 -0.3 1,551,735 + Pf6N2E2_1317 0.86 -2.4 1,551,906 + +0.9 1,551,907 - +0.3 1,552,131 - +1.3 1,552,133 + +0.3 1,552,228 + +1.1 1,552,228 + +0.1 1,552,229 - +0.7 1,552,229 - -0.5 1,552,229 - +0.7 1,552,231 + -0.1 1,552,249 + +1.3 1,552,249 + +0.5 1,552,428 + Pf6N2E2_1318 0.17 -0.8 1,552,549 + Pf6N2E2_1318 0.27 +0.4 1,552,585 + Pf6N2E2_1318 0.29 -0.1 1,552,586 - Pf6N2E2_1318 0.29 -0.8 1,552,653 + Pf6N2E2_1318 0.35 +1.0 1,552,717 + Pf6N2E2_1318 0.39 -1.7
Or see this region's nucleotide sequence