Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_226 and HP15_228 are separated by 6 nucleotides HP15_228 and HP15_227 overlap by 2 nucleotides HP15_227 and HP15_229 are separated by 256 nucleotides HP15_229 and HP15_230 are separated by 64 nucleotides
HP15_226: HP15_226 - ABC transporter ATP-binding protein-like protein, at 211,174 to 213,117
_226
HP15_228: HP15_228 - guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase, at 213,124 to 214,566
_228
HP15_227: HP15_227 - hypothetical protein, at 214,565 to 214,669
_227
HP15_229: HP15_229 - thioredoxin, at 214,926 to 215,270
_229
HP15_230: HP15_230 - molybdenum cofactor biosynthesis protein E, at 215,335 to 215,781
_230
Position (kb) 213
214
215 Strain fitness (log2 ratio) -1
0
1
2
3 at 212.162 kb on + strand, within HP15_226 at 212.162 kb on + strand, within HP15_226 at 212.162 kb on + strand, within HP15_226 at 212.162 kb on + strand, within HP15_226 at 212.162 kb on + strand, within HP15_226 at 212.170 kb on - strand, within HP15_226 at 212.170 kb on - strand, within HP15_226 at 212.170 kb on - strand, within HP15_226 at 212.170 kb on - strand, within HP15_226 at 212.202 kb on + strand, within HP15_226 at 212.216 kb on - strand, within HP15_226 at 212.224 kb on - strand, within HP15_226 at 212.225 kb on + strand, within HP15_226 at 212.228 kb on - strand, within HP15_226 at 212.244 kb on + strand, within HP15_226 at 212.282 kb on + strand, within HP15_226 at 212.359 kb on - strand, within HP15_226 at 212.387 kb on + strand, within HP15_226 at 212.511 kb on + strand, within HP15_226 at 212.516 kb on + strand, within HP15_226 at 212.516 kb on + strand, within HP15_226 at 212.516 kb on + strand, within HP15_226 at 212.590 kb on + strand, within HP15_226 at 212.669 kb on - strand, within HP15_226 at 212.694 kb on + strand, within HP15_226 at 212.760 kb on + strand, within HP15_226 at 212.760 kb on + strand, within HP15_226 at 212.761 kb on + strand, within HP15_226 at 212.768 kb on - strand, within HP15_226 at 212.788 kb on - strand, within HP15_226 at 212.802 kb on - strand, within HP15_226 at 212.822 kb on - strand, within HP15_226 at 212.966 kb on + strand at 212.989 kb on + strand at 213.019 kb on + strand at 213.059 kb on - strand at 213.073 kb on - strand at 213.120 kb on + strand at 213.159 kb on - strand at 213.172 kb on - strand at 213.191 kb on + strand at 213.232 kb on + strand at 213.271 kb on + strand, within HP15_228 at 213.297 kb on + strand, within HP15_228 at 213.343 kb on - strand, within HP15_228 at 213.364 kb on + strand, within HP15_228 at 213.462 kb on - strand, within HP15_228 at 213.467 kb on - strand, within HP15_228 at 213.542 kb on + strand, within HP15_228 at 213.550 kb on - strand, within HP15_228 at 213.555 kb on - strand, within HP15_228 at 213.555 kb on - strand, within HP15_228 at 213.555 kb on - strand, within HP15_228 at 213.582 kb on - strand, within HP15_228 at 213.672 kb on + strand, within HP15_228 at 213.858 kb on - strand, within HP15_228 at 213.872 kb on + strand, within HP15_228 at 213.921 kb on + strand, within HP15_228 at 213.924 kb on + strand, within HP15_228 at 213.929 kb on - strand, within HP15_228 at 214.034 kb on + strand, within HP15_228 at 214.060 kb on + strand, within HP15_228 at 214.242 kb on + strand, within HP15_228 at 214.254 kb on + strand, within HP15_228 at 214.289 kb on + strand, within HP15_228 at 214.332 kb on - strand, within HP15_228 at 214.406 kb on + strand, within HP15_228 at 214.414 kb on - strand, within HP15_228 at 214.414 kb on - strand, within HP15_228 at 214.446 kb on - strand at 214.651 kb on - strand, within HP15_227 at 214.743 kb on + strand at 214.767 kb on - strand at 214.778 kb on + strand at 214.802 kb on + strand at 214.871 kb on - strand at 215.032 kb on - strand, within HP15_229 at 215.397 kb on - strand, within HP15_230 at 215.441 kb on - strand, within HP15_230 at 215.482 kb on - strand, within HP15_230 at 215.486 kb on + strand, within HP15_230 at 215.500 kb on + strand, within HP15_230 at 215.523 kb on + strand, within HP15_230 at 215.531 kb on - strand, within HP15_230 at 215.531 kb on - strand, within HP15_230 at 215.531 kb on - strand, within HP15_230
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 212,162 + HP15_226 0.51 +0.4 212,162 + HP15_226 0.51 +1.0 212,162 + HP15_226 0.51 +0.6 212,162 + HP15_226 0.51 +0.4 212,162 + HP15_226 0.51 -0.0 212,170 - HP15_226 0.51 +0.3 212,170 - HP15_226 0.51 +0.1 212,170 - HP15_226 0.51 -0.0 212,170 - HP15_226 0.51 -0.2 212,202 + HP15_226 0.53 +0.4 212,216 - HP15_226 0.54 -0.4 212,224 - HP15_226 0.54 +0.1 212,225 + HP15_226 0.54 +0.4 212,228 - HP15_226 0.54 -0.7 212,244 + HP15_226 0.55 +0.3 212,282 + HP15_226 0.57 +0.1 212,359 - HP15_226 0.61 -0.7 212,387 + HP15_226 0.62 -0.3 212,511 + HP15_226 0.69 -0.6 212,516 + HP15_226 0.69 +0.8 212,516 + HP15_226 0.69 +0.2 212,516 + HP15_226 0.69 +1.2 212,590 + HP15_226 0.73 +0.1 212,669 - HP15_226 0.77 +1.7 212,694 + HP15_226 0.78 +1.3 212,760 + HP15_226 0.82 -0.7 212,760 + HP15_226 0.82 +1.3 212,761 + HP15_226 0.82 -0.2 212,768 - HP15_226 0.82 -0.5 212,788 - HP15_226 0.83 +0.1 212,802 - HP15_226 0.84 +0.6 212,822 - HP15_226 0.85 +0.6 212,966 + -0.1 212,989 + +0.4 213,019 + +0.3 213,059 - -0.3 213,073 - +3.4 213,120 + +1.0 213,159 - +1.9 213,172 - +0.8 213,191 + +1.0 213,232 + +1.6 213,271 + HP15_228 0.10 +0.8 213,297 + HP15_228 0.12 +1.5 213,343 - HP15_228 0.15 +0.9 213,364 + HP15_228 0.17 +1.4 213,462 - HP15_228 0.23 +1.8 213,467 - HP15_228 0.24 +2.2 213,542 + HP15_228 0.29 +1.2 213,550 - HP15_228 0.30 +0.8 213,555 - HP15_228 0.30 +0.9 213,555 - HP15_228 0.30 +1.5 213,555 - HP15_228 0.30 +2.5 213,582 - HP15_228 0.32 +1.8 213,672 + HP15_228 0.38 +1.4 213,858 - HP15_228 0.51 +1.6 213,872 + HP15_228 0.52 +0.5 213,921 + HP15_228 0.55 +2.0 213,924 + HP15_228 0.55 +1.9 213,929 - HP15_228 0.56 +2.0 214,034 + HP15_228 0.63 +1.5 214,060 + HP15_228 0.65 +0.8 214,242 + HP15_228 0.77 +2.2 214,254 + HP15_228 0.78 +2.1 214,289 + HP15_228 0.81 +2.4 214,332 - HP15_228 0.84 +2.1 214,406 + HP15_228 0.89 +2.7 214,414 - HP15_228 0.89 +1.2 214,414 - HP15_228 0.89 +1.7 214,446 - +2.0 214,651 - HP15_227 0.82 +0.0 214,743 + -0.0 214,767 - +0.4 214,778 + +0.3 214,802 + +0.2 214,871 - -1.3 215,032 - HP15_229 0.31 -0.3 215,397 - HP15_230 0.14 -0.1 215,441 - HP15_230 0.24 +0.2 215,482 - HP15_230 0.33 +3.0 215,486 + HP15_230 0.34 +0.3 215,500 + HP15_230 0.37 +0.2 215,523 + HP15_230 0.42 +0.0 215,531 - HP15_230 0.44 -0.1 215,531 - HP15_230 0.44 -0.4 215,531 - HP15_230 0.44 -0.2
Or see this region's nucleotide sequence