Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1821 and HP15_1822 are separated by 34 nucleotides HP15_1822 and HP15_1823 are separated by 91 nucleotides HP15_1823 and HP15_1824 are separated by 123 nucleotides
HP15_1821: HP15_1821 - amidophosphoribosyltransferase, at 1,917,859 to 1,919,385
_1821
HP15_1822: HP15_1822 - CvpA family protein, at 1,919,420 to 1,919,989
_1822
HP15_1823: HP15_1823 - sporulation domain protein, at 1,920,081 to 1,920,752
_1823
HP15_1824: HP15_1824 - bifunctional protein includes folylpolyglutamate synthase (FPGS) dihydrofolate synthase, at 1,920,876 to 1,922,198
_1824
Position (kb) 1919
1920 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1919.439 kb on + strand at 1919.533 kb on + strand, within HP15_1822 at 1919.551 kb on - strand, within HP15_1822 at 1919.825 kb on - strand, within HP15_1822 at 1919.863 kb on + strand, within HP15_1822 at 1919.871 kb on - strand, within HP15_1822 at 1920.134 kb on - strand at 1920.565 kb on - strand, within HP15_1823 at 1920.805 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 1,919,439 + -3.0 1,919,533 + HP15_1822 0.20 -2.4 1,919,551 - HP15_1822 0.23 +0.1 1,919,825 - HP15_1822 0.71 -0.6 1,919,863 + HP15_1822 0.78 -2.2 1,919,871 - HP15_1822 0.79 -0.8 1,920,134 - +0.0 1,920,565 - HP15_1823 0.72 -0.6 1,920,805 + +0.6
Or see this region's nucleotide sequence