Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_125 and HP15_126 are separated by 68 nucleotides HP15_126 and HP15_127 are separated by 27 nucleotides HP15_127 and HP15_128 overlap by 14 nucleotides HP15_128 and HP15_129 are separated by 57 nucleotides
HP15_125: HP15_125 - conserved hypothetical protein, at 134,272 to 134,613
_125
HP15_126: HP15_126 - single-strand DNA-binding protein, at 134,682 to 135,080
_126
HP15_127: HP15_127 - hypothetical protein, at 135,108 to 135,353
_127
HP15_128: HP15_128 - conserved hypothetical protein, at 135,340 to 135,660
_128
HP15_129: HP15_129 - RecT protein, at 135,718 to 136,674
_129
Position (kb) 135
136 Strain fitness (log2 ratio) -2
-1
0
1 at 134.409 kb on - strand, within HP15_125 at 134.421 kb on - strand, within HP15_125 at 134.476 kb on - strand, within HP15_125 at 134.673 kb on - strand at 134.733 kb on + strand, within HP15_126 at 134.791 kb on - strand, within HP15_126 at 134.791 kb on - strand, within HP15_126 at 134.820 kb on - strand, within HP15_126 at 134.881 kb on + strand, within HP15_126 at 134.896 kb on - strand, within HP15_126 at 134.939 kb on - strand, within HP15_126 at 134.986 kb on + strand, within HP15_126 at 135.096 kb on + strand at 135.216 kb on - strand, within HP15_127 at 135.278 kb on + strand, within HP15_127 at 135.399 kb on + strand, within HP15_128 at 135.402 kb on - strand, within HP15_128 at 135.435 kb on + strand, within HP15_128 at 135.435 kb on + strand, within HP15_128 at 135.504 kb on + strand, within HP15_128 at 135.505 kb on - strand, within HP15_128 at 135.526 kb on + strand, within HP15_128 at 135.559 kb on - strand, within HP15_128 at 135.569 kb on + strand, within HP15_128 at 135.577 kb on - strand, within HP15_128 at 135.633 kb on - strand at 135.641 kb on - strand at 135.662 kb on + strand at 135.720 kb on - strand at 135.739 kb on + strand at 135.757 kb on + strand at 135.757 kb on + strand at 135.851 kb on + strand, within HP15_129 at 135.909 kb on - strand, within HP15_129 at 135.993 kb on + strand, within HP15_129 at 136.067 kb on - strand, within HP15_129 at 136.129 kb on + strand, within HP15_129 at 136.148 kb on + strand, within HP15_129 at 136.404 kb on + strand, within HP15_129 at 136.409 kb on + strand, within HP15_129 at 136.501 kb on + strand, within HP15_129 at 136.501 kb on - strand, within HP15_129 at 136.506 kb on - strand, within HP15_129 at 136.509 kb on - strand, within HP15_129 at 136.509 kb on - strand, within HP15_129 at 136.511 kb on - strand, within HP15_129 at 136.511 kb on - strand, within HP15_129 at 136.511 kb on - strand, within HP15_129 at 136.600 kb on + strand at 136.605 kb on + strand at 136.613 kb on - strand at 136.615 kb on - strand at 136.626 kb on + strand at 136.646 kb on - strand at 136.651 kb on - strand at 136.656 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 134,409 - HP15_125 0.40 -0.1 134,421 - HP15_125 0.44 -0.3 134,476 - HP15_125 0.60 -1.3 134,673 - -0.2 134,733 + HP15_126 0.13 -0.4 134,791 - HP15_126 0.27 +0.2 134,791 - HP15_126 0.27 -0.5 134,820 - HP15_126 0.35 -0.0 134,881 + HP15_126 0.50 -0.1 134,896 - HP15_126 0.54 +0.0 134,939 - HP15_126 0.64 -1.3 134,986 + HP15_126 0.76 +0.5 135,096 + +0.3 135,216 - HP15_127 0.44 +0.4 135,278 + HP15_127 0.69 -1.0 135,399 + HP15_128 0.18 +1.0 135,402 - HP15_128 0.19 +0.4 135,435 + HP15_128 0.30 +0.3 135,435 + HP15_128 0.30 -0.3 135,504 + HP15_128 0.51 -1.0 135,505 - HP15_128 0.51 +0.1 135,526 + HP15_128 0.58 -0.1 135,559 - HP15_128 0.68 +0.4 135,569 + HP15_128 0.71 +1.2 135,577 - HP15_128 0.74 +0.6 135,633 - +0.6 135,641 - +1.2 135,662 + +0.7 135,720 - +0.2 135,739 + +0.4 135,757 + +0.0 135,757 + -0.1 135,851 + HP15_129 0.14 +0.1 135,909 - HP15_129 0.20 +0.3 135,993 + HP15_129 0.29 +0.4 136,067 - HP15_129 0.36 +0.6 136,129 + HP15_129 0.43 -0.7 136,148 + HP15_129 0.45 +0.1 136,404 + HP15_129 0.72 +0.5 136,409 + HP15_129 0.72 -0.3 136,501 + HP15_129 0.82 -0.0 136,501 - HP15_129 0.82 +0.0 136,506 - HP15_129 0.82 +0.7 136,509 - HP15_129 0.83 +0.2 136,509 - HP15_129 0.83 -0.1 136,511 - HP15_129 0.83 +0.1 136,511 - HP15_129 0.83 +0.4 136,511 - HP15_129 0.83 +0.0 136,600 + -1.3 136,605 + +0.6 136,613 - -0.3 136,615 - +0.1 136,626 + -0.7 136,646 - -1.9 136,651 - -0.7 136,656 - -0.1
Or see this region's nucleotide sequence