Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4704 and BT4705 overlap by 27 nucleotides BT4705 and BT4706 are separated by 224 nucleotides BT4706 and BT4707 are separated by 249 nucleotides
BT4704: BT4704 - O-succinylbenzoic acid--CoA ligase (NCBI ptt file), at 6,173,871 to 6,174,977
BT4704
BT4705: BT4705 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 6,174,951 to 6,175,520
BT4705
BT4706: BT4706 - putative anti-sigma factor (NCBI ptt file), at 6,175,745 to 6,176,668
BT4706
BT4707: BT4707 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 6,176,918 to 6,180,217
BT4707
Position (kb)
6175
6176
6177 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6174.815 kb on - strand, within BT4704 at 6174.831 kb on - strand, within BT4704 at 6174.855 kb on + strand, within BT4704 at 6174.856 kb on - strand, within BT4704 at 6174.859 kb on - strand, within BT4704 at 6174.873 kb on - strand at 6174.873 kb on - strand at 6174.917 kb on + strand at 6174.940 kb on + strand at 6174.952 kb on + strand at 6175.040 kb on - strand, within BT4705 at 6175.040 kb on - strand, within BT4705 at 6175.047 kb on - strand, within BT4705 at 6175.119 kb on - strand, within BT4705 at 6175.148 kb on + strand, within BT4705 at 6175.177 kb on - strand, within BT4705 at 6175.191 kb on - strand, within BT4705 at 6175.194 kb on + strand, within BT4705 at 6175.194 kb on + strand, within BT4705 at 6175.195 kb on - strand, within BT4705 at 6175.195 kb on - strand, within BT4705 at 6175.250 kb on - strand, within BT4705 at 6175.311 kb on + strand, within BT4705 at 6175.312 kb on - strand, within BT4705 at 6175.312 kb on - strand, within BT4705 at 6175.384 kb on - strand, within BT4705 at 6175.384 kb on - strand, within BT4705 at 6175.559 kb on - strand at 6175.565 kb on + strand at 6175.636 kb on - strand at 6175.667 kb on + strand at 6175.701 kb on + strand at 6175.727 kb on - strand at 6175.791 kb on + strand at 6175.819 kb on + strand at 6175.828 kb on - strand at 6175.882 kb on + strand, within BT4706 at 6175.883 kb on - strand, within BT4706 at 6175.883 kb on - strand, within BT4706 at 6175.889 kb on - strand, within BT4706 at 6175.889 kb on - strand, within BT4706 at 6175.923 kb on + strand, within BT4706 at 6175.923 kb on + strand, within BT4706 at 6175.924 kb on - strand, within BT4706 at 6175.960 kb on - strand, within BT4706 at 6175.960 kb on - strand, within BT4706 at 6176.080 kb on - strand, within BT4706 at 6176.205 kb on - strand, within BT4706 at 6176.248 kb on + strand, within BT4706 at 6176.249 kb on - strand, within BT4706 at 6176.249 kb on - strand, within BT4706 at 6176.249 kb on - strand, within BT4706 at 6176.252 kb on - strand, within BT4706 at 6176.252 kb on - strand, within BT4706 at 6176.252 kb on - strand, within BT4706 at 6176.256 kb on + strand, within BT4706 at 6176.290 kb on + strand, within BT4706 at 6176.350 kb on + strand, within BT4706 at 6176.356 kb on - strand, within BT4706 at 6176.482 kb on - strand, within BT4706 at 6176.482 kb on - strand, within BT4706 at 6176.497 kb on + strand, within BT4706 at 6176.519 kb on - strand, within BT4706 at 6176.522 kb on + strand, within BT4706 at 6176.560 kb on - strand, within BT4706 at 6176.652 kb on + strand at 6176.663 kb on + strand at 6176.670 kb on - strand at 6176.687 kb on + strand at 6176.692 kb on + strand at 6176.692 kb on + strand at 6176.819 kb on + strand at 6176.877 kb on + strand at 6176.878 kb on - strand at 6176.885 kb on - strand at 6176.918 kb on - strand at 6176.956 kb on - strand at 6177.024 kb on + strand at 6177.033 kb on + strand at 6177.147 kb on + strand at 6177.175 kb on + strand at 6177.180 kb on - strand at 6177.180 kb on - strand at 6177.203 kb on - strand at 6177.214 kb on + strand at 6177.218 kb on - strand at 6177.256 kb on - strand, within BT4707 at 6177.323 kb on - strand, within BT4707 at 6177.345 kb on + strand, within BT4707 at 6177.364 kb on - strand, within BT4707 at 6177.408 kb on - strand, within BT4707 at 6177.424 kb on - strand, within BT4707 at 6177.451 kb on + strand, within BT4707 at 6177.452 kb on - strand, within BT4707 at 6177.536 kb on + strand, within BT4707 at 6177.553 kb on - strand, within BT4707 at 6177.599 kb on - strand, within BT4707 at 6177.626 kb on - strand, within BT4707
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 6,174,815 - BT4704 0.85 -2.9 6,174,831 - BT4704 0.87 -0.1 6,174,855 + BT4704 0.89 -1.1 6,174,856 - BT4704 0.89 -0.1 6,174,859 - BT4704 0.89 -2.4 6,174,873 - -1.7 6,174,873 - -1.7 6,174,917 + -1.7 6,174,940 + -0.1 6,174,952 + -1.8 6,175,040 - BT4705 0.16 +0.7 6,175,040 - BT4705 0.16 -0.7 6,175,047 - BT4705 0.17 -0.9 6,175,119 - BT4705 0.29 -0.2 6,175,148 + BT4705 0.35 -1.3 6,175,177 - BT4705 0.40 -0.7 6,175,191 - BT4705 0.42 -0.7 6,175,194 + BT4705 0.43 -0.8 6,175,194 + BT4705 0.43 -0.1 6,175,195 - BT4705 0.43 +0.1 6,175,195 - BT4705 0.43 -1.4 6,175,250 - BT4705 0.52 +2.7 6,175,311 + BT4705 0.63 +2.2 6,175,312 - BT4705 0.63 -1.5 6,175,312 - BT4705 0.63 -3.5 6,175,384 - BT4705 0.76 -1.2 6,175,384 - BT4705 0.76 -2.4 6,175,559 - +2.9 6,175,565 + -1.1 6,175,636 - -0.1 6,175,667 + -2.1 6,175,701 + -1.1 6,175,727 - +2.3 6,175,791 + -1.3 6,175,819 + -1.3 6,175,828 - -1.5 6,175,882 + BT4706 0.15 +0.9 6,175,883 - BT4706 0.15 -0.1 6,175,883 - BT4706 0.15 -0.1 6,175,889 - BT4706 0.16 +2.7 6,175,889 - BT4706 0.16 -2.1 6,175,923 + BT4706 0.19 -0.9 6,175,923 + BT4706 0.19 -0.1 6,175,924 - BT4706 0.19 -2.9 6,175,960 - BT4706 0.23 -0.1 6,175,960 - BT4706 0.23 -0.1 6,176,080 - BT4706 0.36 +2.3 6,176,205 - BT4706 0.50 -3.4 6,176,248 + BT4706 0.54 -0.3 6,176,249 - BT4706 0.55 -1.1 6,176,249 - BT4706 0.55 -0.1 6,176,249 - BT4706 0.55 +2.0 6,176,252 - BT4706 0.55 +1.1 6,176,252 - BT4706 0.55 -1.7 6,176,252 - BT4706 0.55 -0.7 6,176,256 + BT4706 0.55 +0.9 6,176,290 + BT4706 0.59 -0.7 6,176,350 + BT4706 0.65 +0.9 6,176,356 - BT4706 0.66 -0.3 6,176,482 - BT4706 0.80 -0.1 6,176,482 - BT4706 0.80 -0.1 6,176,497 + BT4706 0.81 -1.1 6,176,519 - BT4706 0.84 +0.5 6,176,522 + BT4706 0.84 +1.5 6,176,560 - BT4706 0.88 +1.9 6,176,652 + -1.1 6,176,663 + -1.1 6,176,670 - -2.4 6,176,687 + +1.9 6,176,692 + +0.9 6,176,692 + -1.1 6,176,819 + +1.5 6,176,877 + -1.4 6,176,878 - -1.7 6,176,885 - -2.9 6,176,918 - -1.3 6,176,956 - -0.4 6,177,024 + -0.2 6,177,033 + -2.3 6,177,147 + -1.7 6,177,175 + +1.9 6,177,180 - -1.4 6,177,180 - +3.0 6,177,203 - -2.4 6,177,214 + -1.5 6,177,218 - +0.9 6,177,256 - BT4707 0.10 -0.5 6,177,323 - BT4707 0.12 +0.5 6,177,345 + BT4707 0.13 +0.7 6,177,364 - BT4707 0.14 +0.5 6,177,408 - BT4707 0.15 -0.8 6,177,424 - BT4707 0.15 +0.9 6,177,451 + BT4707 0.16 -3.2 6,177,452 - BT4707 0.16 -1.2 6,177,536 + BT4707 0.19 -0.5 6,177,553 - BT4707 0.19 -1.1 6,177,599 - BT4707 0.21 -0.6 6,177,626 - BT4707 0.21 -1.1
Or see this region's nucleotide sequence