Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4146 and BT4147 are separated by 89 nucleotides BT4147 and BT4148 overlap by 50 nucleotides BT4148 and BT4149 are separated by 245 nucleotides
BT4146: BT4146 - exo-poly-alpha-D-galacturonosidase precursor (NCBI ptt file), at 5,444,165 to 5,445,568
BT4146
BT4147: BT4147 - hypothetical protein (NCBI ptt file), at 5,445,658 to 5,446,248
BT4147
BT4148: BT4148 - hypothetical protein (NCBI ptt file), at 5,446,199 to 5,446,414
BT4148
BT4149: BT4149 - exo-poly-alpha-D-galacturonosidase precursor (NCBI ptt file), at 5,446,660 to 5,447,718
BT4149
Position (kb)
5445
5446
5447 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5444.832 kb on + strand, within BT4146 at 5444.945 kb on + strand, within BT4146 at 5444.990 kb on + strand, within BT4146 at 5445.020 kb on + strand, within BT4146 at 5445.353 kb on - strand, within BT4146 at 5445.401 kb on + strand, within BT4146 at 5445.502 kb on + strand at 5445.504 kb on + strand at 5445.551 kb on + strand at 5445.556 kb on - strand at 5445.645 kb on - strand at 5445.648 kb on - strand at 5445.676 kb on - strand at 5445.717 kb on - strand, within BT4147 at 5445.955 kb on + strand, within BT4147 at 5445.971 kb on - strand, within BT4147 at 5445.972 kb on + strand, within BT4147 at 5446.014 kb on + strand, within BT4147 at 5446.014 kb on + strand, within BT4147 at 5446.150 kb on - strand, within BT4147 at 5446.333 kb on + strand, within BT4148 at 5446.333 kb on + strand, within BT4148 at 5446.345 kb on + strand, within BT4148 at 5446.418 kb on - strand at 5446.452 kb on - strand at 5446.479 kb on + strand at 5446.481 kb on + strand at 5446.482 kb on - strand at 5446.482 kb on - strand at 5446.590 kb on + strand at 5446.590 kb on + strand at 5446.666 kb on - strand at 5446.669 kb on + strand at 5446.721 kb on + strand at 5446.721 kb on + strand at 5446.750 kb on + strand at 5446.753 kb on + strand at 5446.753 kb on + strand at 5446.753 kb on + strand at 5446.861 kb on + strand, within BT4149 at 5446.896 kb on + strand, within BT4149 at 5446.915 kb on + strand, within BT4149 at 5446.936 kb on + strand, within BT4149 at 5447.174 kb on + strand, within BT4149 at 5447.233 kb on - strand, within BT4149
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 5,444,832 + BT4146 0.48 -1.1 5,444,945 + BT4146 0.56 -1.1 5,444,990 + BT4146 0.59 -0.7 5,445,020 + BT4146 0.61 +0.3 5,445,353 - BT4146 0.85 -0.2 5,445,401 + BT4146 0.88 +1.7 5,445,502 + -2.0 5,445,504 + -1.5 5,445,551 + -2.2 5,445,556 - +1.1 5,445,645 - -3.8 5,445,648 - -0.9 5,445,676 - -0.2 5,445,717 - BT4147 0.10 -3.6 5,445,955 + BT4147 0.50 -2.2 5,445,971 - BT4147 0.53 +0.5 5,445,972 + BT4147 0.53 -1.9 5,446,014 + BT4147 0.60 +2.7 5,446,014 + BT4147 0.60 -1.9 5,446,150 - BT4147 0.83 -1.5 5,446,333 + BT4148 0.62 +1.4 5,446,333 + BT4148 0.62 +0.7 5,446,345 + BT4148 0.68 -0.7 5,446,418 - +0.1 5,446,452 - -1.3 5,446,479 + +1.7 5,446,481 + -0.9 5,446,482 - +2.2 5,446,482 - -1.2 5,446,590 + -2.3 5,446,590 + -1.8 5,446,666 - -1.2 5,446,669 + -1.0 5,446,721 + +0.1 5,446,721 + -1.3 5,446,750 + -1.9 5,446,753 + +1.9 5,446,753 + -0.2 5,446,753 + +1.9 5,446,861 + BT4149 0.19 +1.2 5,446,896 + BT4149 0.22 +0.1 5,446,915 + BT4149 0.24 -2.7 5,446,936 + BT4149 0.26 -2.7 5,447,174 + BT4149 0.49 +1.4 5,447,233 - BT4149 0.54 +0.7
Or see this region's nucleotide sequence