Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3657 and BT3658 are separated by 289 nucleotides BT3658 and BT3659 are separated by 405 nucleotides BT3659 and BT3660 are separated by 84 nucleotides
BT3657: BT3657 - alpha-L-arabinofuranosidase A precursor (NCBI ptt file), at 4,738,696 to 4,741,173
BT3657
BT3658: BT3658 - hypothetical protein (NCBI ptt file), at 4,741,463 to 4,741,675
BT3658
BT3659: BT3659 - alpha-xylosidase (NCBI ptt file), at 4,742,081 to 4,744,555
BT3659
BT3660: BT3660 - transcriptional regulator (NCBI ptt file), at 4,744,640 to 4,747,354
BT3660
Position (kb)
4742
4743
4744
4745 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 4741.172 kb on - strand at 4741.193 kb on + strand at 4741.193 kb on + strand at 4741.194 kb on - strand at 4741.199 kb on - strand at 4741.222 kb on + strand at 4741.455 kb on - strand at 4741.462 kb on - strand at 4741.494 kb on + strand, within BT3658 at 4741.494 kb on + strand, within BT3658 at 4741.531 kb on + strand, within BT3658 at 4741.532 kb on - strand, within BT3658 at 4741.532 kb on - strand, within BT3658 at 4741.548 kb on - strand, within BT3658 at 4741.555 kb on - strand, within BT3658 at 4741.565 kb on - strand, within BT3658 at 4741.565 kb on - strand, within BT3658 at 4741.573 kb on - strand, within BT3658 at 4741.575 kb on + strand, within BT3658 at 4741.614 kb on - strand, within BT3658 at 4741.625 kb on - strand, within BT3658 at 4741.630 kb on + strand, within BT3658 at 4741.631 kb on - strand, within BT3658 at 4741.647 kb on + strand, within BT3658 at 4741.775 kb on - strand at 4741.776 kb on + strand at 4741.777 kb on - strand at 4741.796 kb on + strand at 4741.834 kb on - strand at 4741.834 kb on - strand at 4741.843 kb on + strand at 4741.844 kb on - strand at 4741.844 kb on - strand at 4741.850 kb on + strand at 4741.851 kb on - strand at 4741.858 kb on - strand at 4741.881 kb on + strand at 4741.882 kb on - strand at 4741.895 kb on + strand at 4741.895 kb on + strand at 4741.895 kb on + strand at 4741.915 kb on + strand at 4741.925 kb on + strand at 4741.986 kb on + strand at 4741.994 kb on - strand at 4742.025 kb on + strand at 4742.026 kb on - strand at 4742.048 kb on - strand at 4742.104 kb on + strand at 4742.112 kb on + strand at 4742.172 kb on - strand at 4742.213 kb on - strand at 4742.214 kb on + strand at 4742.215 kb on - strand at 4742.215 kb on - strand at 4742.245 kb on + strand at 4742.245 kb on + strand at 4742.283 kb on + strand at 4742.302 kb on + strand at 4742.302 kb on + strand at 4742.303 kb on - strand at 4742.303 kb on - strand at 4742.303 kb on - strand at 4742.305 kb on - strand at 4742.305 kb on - strand at 4742.360 kb on - strand, within BT3659 at 4742.360 kb on - strand, within BT3659 at 4742.443 kb on - strand, within BT3659 at 4742.453 kb on - strand, within BT3659 at 4742.545 kb on - strand, within BT3659 at 4742.545 kb on - strand, within BT3659 at 4742.545 kb on - strand, within BT3659 at 4742.612 kb on - strand, within BT3659 at 4742.614 kb on - strand, within BT3659 at 4742.614 kb on - strand, within BT3659 at 4742.662 kb on - strand, within BT3659 at 4742.763 kb on + strand, within BT3659 at 4742.763 kb on + strand, within BT3659 at 4742.769 kb on + strand, within BT3659 at 4742.770 kb on - strand, within BT3659 at 4742.790 kb on - strand, within BT3659 at 4742.800 kb on - strand, within BT3659 at 4742.856 kb on - strand, within BT3659 at 4742.951 kb on + strand, within BT3659 at 4742.952 kb on - strand, within BT3659 at 4742.962 kb on + strand, within BT3659 at 4742.963 kb on - strand, within BT3659 at 4742.965 kb on - strand, within BT3659 at 4743.024 kb on + strand, within BT3659 at 4743.025 kb on - strand, within BT3659 at 4743.025 kb on - strand, within BT3659 at 4743.042 kb on - strand, within BT3659 at 4743.102 kb on + strand, within BT3659 at 4743.102 kb on + strand, within BT3659 at 4743.143 kb on + strand, within BT3659 at 4743.882 kb on + strand, within BT3659 at 4743.894 kb on + strand, within BT3659 at 4743.911 kb on + strand, within BT3659 at 4743.911 kb on + strand, within BT3659 at 4743.959 kb on - strand, within BT3659 at 4743.959 kb on - strand, within BT3659 at 4743.967 kb on - strand, within BT3659 at 4743.979 kb on - strand, within BT3659 at 4743.988 kb on - strand, within BT3659 at 4744.021 kb on + strand, within BT3659 at 4744.022 kb on - strand, within BT3659 at 4744.022 kb on - strand, within BT3659 at 4744.099 kb on - strand, within BT3659 at 4744.143 kb on + strand, within BT3659 at 4744.268 kb on + strand, within BT3659 at 4744.390 kb on - strand at 4744.449 kb on + strand at 4744.564 kb on + strand at 4744.574 kb on - strand at 4744.574 kb on - strand at 4744.616 kb on + strand at 4744.712 kb on + strand at 4744.896 kb on - strand at 4744.946 kb on + strand, within BT3660 at 4745.086 kb on - strand, within BT3660 at 4745.140 kb on + strand, within BT3660 at 4745.176 kb on + strand, within BT3660 at 4745.178 kb on + strand, within BT3660 at 4745.179 kb on - strand, within BT3660 at 4745.208 kb on - strand, within BT3660 at 4745.291 kb on - strand, within BT3660 at 4745.303 kb on + strand, within BT3660 at 4745.304 kb on - strand, within BT3660 at 4745.338 kb on - strand, within BT3660 at 4745.361 kb on + strand, within BT3660 at 4745.362 kb on - strand, within BT3660 at 4745.526 kb on + strand, within BT3660
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 4,741,172 - -1.9 4,741,193 + +0.6 4,741,193 + +2.5 4,741,194 - -2.3 4,741,199 - +1.7 4,741,222 + -1.9 4,741,455 - -0.7 4,741,462 - +0.1 4,741,494 + BT3658 0.15 -0.7 4,741,494 + BT3658 0.15 +4.0 4,741,531 + BT3658 0.32 +1.1 4,741,532 - BT3658 0.32 -0.9 4,741,532 - BT3658 0.32 -1.7 4,741,548 - BT3658 0.40 -0.4 4,741,555 - BT3658 0.43 -1.9 4,741,565 - BT3658 0.48 -0.6 4,741,565 - BT3658 0.48 +0.1 4,741,573 - BT3658 0.52 -1.3 4,741,575 + BT3658 0.53 -1.2 4,741,614 - BT3658 0.71 -1.5 4,741,625 - BT3658 0.76 +0.1 4,741,630 + BT3658 0.78 -2.3 4,741,631 - BT3658 0.79 -1.2 4,741,647 + BT3658 0.86 -0.4 4,741,775 - -1.4 4,741,776 + -2.2 4,741,777 - -3.7 4,741,796 + -2.2 4,741,834 - -0.9 4,741,834 - -1.1 4,741,843 + -2.0 4,741,844 - +1.4 4,741,844 - +0.5 4,741,850 + -3.6 4,741,851 - -0.9 4,741,858 - -1.9 4,741,881 + -0.9 4,741,882 - +0.1 4,741,895 + -1.1 4,741,895 + -2.0 4,741,895 + -1.2 4,741,915 + +3.7 4,741,925 + -2.1 4,741,986 + +0.6 4,741,994 - -0.9 4,742,025 + -1.3 4,742,026 - -0.4 4,742,048 - -1.9 4,742,104 + +0.1 4,742,112 + -1.3 4,742,172 - -2.0 4,742,213 - -2.7 4,742,214 + -2.4 4,742,215 - +0.5 4,742,215 - -0.2 4,742,245 + -1.0 4,742,245 + -1.4 4,742,283 + +1.7 4,742,302 + -3.7 4,742,302 + -2.2 4,742,303 - -2.9 4,742,303 - -1.4 4,742,303 - +1.4 4,742,305 - +2.1 4,742,305 - -1.4 4,742,360 - BT3659 0.11 -2.4 4,742,360 - BT3659 0.11 +3.1 4,742,443 - BT3659 0.15 -0.7 4,742,453 - BT3659 0.15 -1.3 4,742,545 - BT3659 0.19 -2.8 4,742,545 - BT3659 0.19 +2.5 4,742,545 - BT3659 0.19 +3.6 4,742,612 - BT3659 0.21 +1.3 4,742,614 - BT3659 0.22 -1.4 4,742,614 - BT3659 0.22 +2.5 4,742,662 - BT3659 0.23 -4.3 4,742,763 + BT3659 0.28 -3.3 4,742,763 + BT3659 0.28 -0.9 4,742,769 + BT3659 0.28 -0.3 4,742,770 - BT3659 0.28 -1.0 4,742,790 - BT3659 0.29 -3.6 4,742,800 - BT3659 0.29 -2.9 4,742,856 - BT3659 0.31 +0.7 4,742,951 + BT3659 0.35 -1.4 4,742,952 - BT3659 0.35 -0.9 4,742,962 + BT3659 0.36 +2.1 4,742,963 - BT3659 0.36 +1.3 4,742,965 - BT3659 0.36 -0.5 4,743,024 + BT3659 0.38 -2.7 4,743,025 - BT3659 0.38 -1.4 4,743,025 - BT3659 0.38 -0.3 4,743,042 - BT3659 0.39 -0.3 4,743,102 + BT3659 0.41 -0.4 4,743,102 + BT3659 0.41 -0.9 4,743,143 + BT3659 0.43 -0.9 4,743,882 + BT3659 0.73 -2.2 4,743,894 + BT3659 0.73 +1.7 4,743,911 + BT3659 0.74 -2.2 4,743,911 + BT3659 0.74 -3.7 4,743,959 - BT3659 0.76 -1.7 4,743,959 - BT3659 0.76 -2.4 4,743,967 - BT3659 0.76 -2.2 4,743,979 - BT3659 0.77 -1.9 4,743,988 - BT3659 0.77 +0.7 4,744,021 + BT3659 0.78 -4.5 4,744,022 - BT3659 0.78 -2.2 4,744,022 - BT3659 0.78 +1.7 4,744,099 - BT3659 0.82 -3.6 4,744,143 + BT3659 0.83 -2.4 4,744,268 + BT3659 0.88 -2.4 4,744,390 - -0.5 4,744,449 + -0.2 4,744,564 + +0.1 4,744,574 - +2.6 4,744,574 - -1.9 4,744,616 + +3.0 4,744,712 + -2.1 4,744,896 - +1.3 4,744,946 + BT3660 0.11 -1.0 4,745,086 - BT3660 0.16 -0.5 4,745,140 + BT3660 0.18 -1.9 4,745,176 + BT3660 0.20 +0.1 4,745,178 + BT3660 0.20 -0.9 4,745,179 - BT3660 0.20 -2.7 4,745,208 - BT3660 0.21 +0.1 4,745,291 - BT3660 0.24 -1.5 4,745,303 + BT3660 0.24 -0.3 4,745,304 - BT3660 0.24 -1.3 4,745,338 - BT3660 0.26 -2.2 4,745,361 + BT3660 0.27 -0.9 4,745,362 - BT3660 0.27 -0.3 4,745,526 + BT3660 0.33 -0.7
Or see this region's nucleotide sequence