Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3469 and BT3470 are separated by 58 nucleotides BT3470 and BT3471 are separated by 93 nucleotides BT3471 and BT3472 overlap by 4 nucleotides
BT3469: BT3469 - conserved hypothetical protein (NCBI ptt file), at 4,467,808 to 4,469,157
BT3469
BT3470: BT3470 - putative dehydrogenase (NCBI ptt file), at 4,469,216 to 4,470,598
BT3470
BT3471: BT3471 - hypothetical protein (NCBI ptt file), at 4,470,692 to 4,470,934
BT3471
BT3472: BT3472 - putative hydrogenase (NCBI ptt file), at 4,470,931 to 4,472,223
BT3472
Position (kb)
4469
4470
4471 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 4468.240 kb on - strand, within BT3469 at 4468.288 kb on + strand, within BT3469 at 4468.288 kb on + strand, within BT3469 at 4468.289 kb on - strand, within BT3469 at 4468.293 kb on + strand, within BT3469 at 4468.293 kb on + strand, within BT3469 at 4468.294 kb on - strand, within BT3469 at 4468.417 kb on - strand, within BT3469 at 4468.429 kb on + strand, within BT3469 at 4468.479 kb on + strand, within BT3469 at 4468.526 kb on - strand, within BT3469 at 4468.537 kb on - strand, within BT3469 at 4468.542 kb on + strand, within BT3469 at 4468.591 kb on + strand, within BT3469 at 4468.592 kb on - strand, within BT3469 at 4468.632 kb on + strand, within BT3469 at 4468.632 kb on + strand, within BT3469 at 4468.633 kb on - strand, within BT3469 at 4468.633 kb on - strand, within BT3469 at 4468.633 kb on - strand, within BT3469 at 4468.633 kb on - strand, within BT3469 at 4468.633 kb on - strand, within BT3469 at 4468.793 kb on + strand, within BT3469 at 4468.794 kb on - strand, within BT3469 at 4468.798 kb on - strand, within BT3469 at 4468.952 kb on + strand, within BT3469 at 4468.953 kb on - strand, within BT3469 at 4468.995 kb on + strand, within BT3469 at 4469.048 kb on - strand at 4469.140 kb on + strand at 4469.186 kb on - strand at 4469.208 kb on - strand at 4469.217 kb on + strand at 4469.293 kb on + strand at 4469.306 kb on - strand at 4469.306 kb on - strand at 4469.363 kb on + strand, within BT3470 at 4469.372 kb on + strand, within BT3470 at 4469.373 kb on - strand, within BT3470 at 4469.414 kb on - strand, within BT3470 at 4469.420 kb on - strand, within BT3470 at 4469.466 kb on - strand, within BT3470 at 4469.475 kb on - strand, within BT3470 at 4469.645 kb on + strand, within BT3470 at 4469.645 kb on + strand, within BT3470 at 4469.701 kb on + strand, within BT3470 at 4469.706 kb on - strand, within BT3470 at 4469.747 kb on - strand, within BT3470 at 4469.747 kb on - strand, within BT3470 at 4469.770 kb on - strand, within BT3470 at 4469.893 kb on - strand, within BT3470 at 4469.893 kb on - strand, within BT3470 at 4469.919 kb on + strand, within BT3470 at 4469.931 kb on + strand, within BT3470 at 4469.932 kb on - strand, within BT3470 at 4470.008 kb on - strand, within BT3470 at 4470.009 kb on + strand, within BT3470 at 4470.046 kb on + strand, within BT3470 at 4470.118 kb on - strand, within BT3470 at 4470.204 kb on + strand, within BT3470 at 4470.234 kb on + strand, within BT3470 at 4470.235 kb on - strand, within BT3470 at 4470.235 kb on - strand, within BT3470 at 4470.236 kb on + strand, within BT3470 at 4470.236 kb on + strand, within BT3470 at 4470.236 kb on + strand, within BT3470 at 4470.237 kb on - strand, within BT3470 at 4470.237 kb on - strand, within BT3470 at 4470.237 kb on - strand, within BT3470 at 4470.260 kb on + strand, within BT3470 at 4470.268 kb on + strand, within BT3470 at 4470.376 kb on - strand at 4470.376 kb on - strand, within BT3470 at 4470.383 kb on + strand, within BT3470 at 4470.441 kb on - strand, within BT3470 at 4470.469 kb on + strand at 4470.494 kb on - strand at 4470.501 kb on + strand at 4470.506 kb on + strand at 4470.507 kb on - strand at 4470.672 kb on + strand at 4470.675 kb on - strand at 4470.679 kb on + strand at 4470.681 kb on + strand at 4470.730 kb on - strand, within BT3471 at 4470.739 kb on + strand, within BT3471 at 4470.743 kb on + strand, within BT3471 at 4470.743 kb on + strand, within BT3471 at 4470.743 kb on + strand, within BT3471 at 4470.762 kb on + strand, within BT3471 at 4470.764 kb on + strand, within BT3471 at 4470.765 kb on - strand, within BT3471 at 4470.765 kb on - strand, within BT3471 at 4470.803 kb on + strand, within BT3471 at 4470.824 kb on - strand, within BT3471 at 4470.828 kb on + strand, within BT3471 at 4470.828 kb on + strand, within BT3471 at 4470.829 kb on - strand, within BT3471 at 4470.837 kb on - strand, within BT3471 at 4470.868 kb on + strand, within BT3471 at 4470.926 kb on - strand at 4470.935 kb on - strand at 4471.045 kb on - strand at 4471.045 kb on - strand at 4471.052 kb on + strand at 4471.053 kb on - strand at 4471.055 kb on + strand at 4471.056 kb on - strand at 4471.095 kb on + strand, within BT3472 at 4471.411 kb on + strand, within BT3472 at 4471.412 kb on - strand, within BT3472 at 4471.415 kb on + strand, within BT3472 at 4471.416 kb on - strand, within BT3472 at 4471.417 kb on + strand, within BT3472 at 4471.426 kb on + strand, within BT3472 at 4471.465 kb on - strand, within BT3472 at 4471.467 kb on + strand, within BT3472 at 4471.467 kb on + strand, within BT3472 at 4471.565 kb on + strand, within BT3472 at 4471.572 kb on + strand, within BT3472
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 4,468,240 - BT3469 0.32 -1.4 4,468,288 + BT3469 0.36 -0.6 4,468,288 + BT3469 0.36 -1.9 4,468,289 - BT3469 0.36 -1.9 4,468,293 + BT3469 0.36 +2.0 4,468,293 + BT3469 0.36 +0.9 4,468,294 - BT3469 0.36 +0.1 4,468,417 - BT3469 0.45 +1.1 4,468,429 + BT3469 0.46 -3.2 4,468,479 + BT3469 0.50 -1.5 4,468,526 - BT3469 0.53 -1.7 4,468,537 - BT3469 0.54 -1.2 4,468,542 + BT3469 0.54 -1.2 4,468,591 + BT3469 0.58 -2.7 4,468,592 - BT3469 0.58 -3.7 4,468,632 + BT3469 0.61 -2.2 4,468,632 + BT3469 0.61 +2.0 4,468,633 - BT3469 0.61 -1.5 4,468,633 - BT3469 0.61 +0.1 4,468,633 - BT3469 0.61 -0.9 4,468,633 - BT3469 0.61 -1.4 4,468,633 - BT3469 0.61 +2.1 4,468,793 + BT3469 0.73 -1.7 4,468,794 - BT3469 0.73 +0.6 4,468,798 - BT3469 0.73 -2.7 4,468,952 + BT3469 0.85 -0.9 4,468,953 - BT3469 0.85 -0.7 4,468,995 + BT3469 0.88 -1.4 4,469,048 - -1.3 4,469,140 + +1.7 4,469,186 - +3.1 4,469,208 - -1.1 4,469,217 + +2.7 4,469,293 + -1.9 4,469,306 - -0.8 4,469,306 - -1.4 4,469,363 + BT3470 0.11 -0.9 4,469,372 + BT3470 0.11 +0.1 4,469,373 - BT3470 0.11 -3.2 4,469,414 - BT3470 0.14 +0.1 4,469,420 - BT3470 0.15 +0.1 4,469,466 - BT3470 0.18 +1.1 4,469,475 - BT3470 0.19 -2.1 4,469,645 + BT3470 0.31 -1.7 4,469,645 + BT3470 0.31 -2.8 4,469,701 + BT3470 0.35 +1.1 4,469,706 - BT3470 0.35 -0.9 4,469,747 - BT3470 0.38 +1.7 4,469,747 - BT3470 0.38 +1.1 4,469,770 - BT3470 0.40 +2.1 4,469,893 - BT3470 0.49 -0.9 4,469,893 - BT3470 0.49 -2.9 4,469,919 + BT3470 0.51 +1.1 4,469,931 + BT3470 0.52 -1.5 4,469,932 - BT3470 0.52 -2.9 4,470,008 - BT3470 0.57 +3.6 4,470,009 + BT3470 0.57 -0.9 4,470,046 + BT3470 0.60 -0.3 4,470,118 - BT3470 0.65 -1.1 4,470,204 + BT3470 0.71 +1.1 4,470,234 + BT3470 0.74 -4.3 4,470,235 - BT3470 0.74 -3.9 4,470,235 - BT3470 0.74 +2.0 4,470,236 + BT3470 0.74 +2.2 4,470,236 + BT3470 0.74 +0.1 4,470,236 + BT3470 0.74 -1.2 4,470,237 - BT3470 0.74 +1.7 4,470,237 - BT3470 0.74 -2.7 4,470,237 - BT3470 0.74 -0.1 4,470,260 + BT3470 0.75 -1.9 4,470,268 + BT3470 0.76 +1.1 4,470,376 - +1.1 4,470,376 - BT3470 0.84 -2.4 4,470,383 + BT3470 0.84 -2.7 4,470,441 - BT3470 0.89 +1.1 4,470,469 + -1.0 4,470,494 - +0.7 4,470,501 + -2.9 4,470,506 + -0.9 4,470,507 - +1.7 4,470,672 + -1.4 4,470,675 - +2.1 4,470,679 + -1.9 4,470,681 + -2.2 4,470,730 - BT3471 0.16 -0.9 4,470,739 + BT3471 0.19 -1.1 4,470,743 + BT3471 0.21 +1.3 4,470,743 + BT3471 0.21 +0.1 4,470,743 + BT3471 0.21 +1.1 4,470,762 + BT3471 0.29 -1.7 4,470,764 + BT3471 0.30 +0.8 4,470,765 - BT3471 0.30 -1.4 4,470,765 - BT3471 0.30 -0.3 4,470,803 + BT3471 0.46 +3.3 4,470,824 - BT3471 0.54 -2.3 4,470,828 + BT3471 0.56 -1.5 4,470,828 + BT3471 0.56 -1.2 4,470,829 - BT3471 0.56 +0.4 4,470,837 - BT3471 0.60 +1.7 4,470,868 + BT3471 0.72 -2.5 4,470,926 - +0.1 4,470,935 - -2.8 4,471,045 - -1.6 4,471,045 - -2.1 4,471,052 + -0.9 4,471,053 - +0.1 4,471,055 + -1.5 4,471,056 - +1.5 4,471,095 + BT3472 0.13 -2.2 4,471,411 + BT3472 0.37 +4.0 4,471,412 - BT3472 0.37 +1.1 4,471,415 + BT3472 0.37 +0.6 4,471,416 - BT3472 0.38 -2.0 4,471,417 + BT3472 0.38 -0.7 4,471,426 + BT3472 0.38 -1.9 4,471,465 - BT3472 0.41 +1.1 4,471,467 + BT3472 0.41 +0.1 4,471,467 + BT3472 0.41 +0.1 4,471,565 + BT3472 0.49 -0.3 4,471,572 + BT3472 0.50 -0.9
Or see this region's nucleotide sequence