Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3300 and BT3301 are separated by 111 nucleotides BT3301 and BT3302 are separated by 369 nucleotides
BT3300: BT3300 - beta-glucosidase (NCBI ptt file), at 4,231,298 to 4,233,775
BT3300
BT3301: BT3301 - putative alpha-1,6-mannanase (NCBI ptt file), at 4,233,887 to 4,235,074
BT3301
BT3302: BT3302 - two-component system sensor histidine kinase/response regulator, hybrid ('one-component system') (NCBI ptt file), at 4,235,444 to 4,239,544
BT3302
Position (kb)
4233
4234
4235
4236 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4232.938 kb on - strand, within BT3300 at 4232.940 kb on + strand, within BT3300 at 4232.947 kb on + strand, within BT3300 at 4232.954 kb on - strand, within BT3300 at 4233.048 kb on - strand at 4233.077 kb on + strand, within BT3300 at 4233.112 kb on - strand, within BT3300 at 4233.133 kb on - strand, within BT3300 at 4233.133 kb on - strand, within BT3300 at 4233.133 kb on - strand, within BT3300 at 4233.178 kb on + strand, within BT3300 at 4233.179 kb on - strand, within BT3300 at 4233.205 kb on - strand, within BT3300 at 4233.226 kb on + strand, within BT3300 at 4233.232 kb on + strand, within BT3300 at 4233.236 kb on - strand, within BT3300 at 4233.302 kb on + strand, within BT3300 at 4233.328 kb on + strand, within BT3300 at 4233.329 kb on - strand, within BT3300 at 4233.338 kb on + strand, within BT3300 at 4233.339 kb on - strand, within BT3300 at 4233.343 kb on - strand, within BT3300 at 4233.359 kb on - strand, within BT3300 at 4233.360 kb on + strand, within BT3300 at 4233.360 kb on + strand, within BT3300 at 4233.361 kb on - strand, within BT3300 at 4233.361 kb on - strand, within BT3300 at 4233.397 kb on + strand, within BT3300 at 4233.398 kb on - strand, within BT3300 at 4233.413 kb on + strand, within BT3300 at 4233.493 kb on + strand, within BT3300 at 4233.609 kb on + strand at 4233.610 kb on - strand at 4233.650 kb on + strand at 4233.668 kb on + strand at 4233.668 kb on + strand at 4233.700 kb on + strand at 4233.702 kb on + strand at 4233.793 kb on + strand at 4233.793 kb on + strand at 4233.812 kb on - strand at 4233.847 kb on + strand at 4233.852 kb on + strand at 4234.023 kb on + strand, within BT3301 at 4234.028 kb on + strand, within BT3301 at 4234.029 kb on - strand, within BT3301 at 4234.097 kb on + strand, within BT3301 at 4234.141 kb on + strand, within BT3301 at 4234.182 kb on + strand, within BT3301 at 4234.183 kb on - strand, within BT3301 at 4234.183 kb on - strand, within BT3301 at 4234.183 kb on - strand, within BT3301 at 4234.307 kb on + strand, within BT3301 at 4234.313 kb on - strand, within BT3301 at 4234.352 kb on - strand, within BT3301 at 4234.381 kb on + strand, within BT3301 at 4234.429 kb on + strand, within BT3301 at 4234.429 kb on + strand, within BT3301 at 4234.448 kb on + strand, within BT3301 at 4234.506 kb on + strand, within BT3301 at 4234.528 kb on + strand, within BT3301 at 4234.607 kb on - strand, within BT3301 at 4234.633 kb on + strand, within BT3301 at 4234.636 kb on + strand, within BT3301 at 4234.693 kb on + strand, within BT3301 at 4234.737 kb on - strand, within BT3301 at 4234.737 kb on - strand, within BT3301 at 4234.762 kb on - strand, within BT3301 at 4234.784 kb on + strand, within BT3301 at 4234.784 kb on + strand, within BT3301 at 4234.818 kb on + strand, within BT3301 at 4234.827 kb on - strand, within BT3301 at 4234.831 kb on - strand, within BT3301 at 4234.843 kb on + strand, within BT3301 at 4234.885 kb on + strand, within BT3301 at 4234.885 kb on + strand, within BT3301 at 4234.951 kb on - strand, within BT3301 at 4235.082 kb on - strand at 4235.084 kb on + strand at 4235.085 kb on - strand at 4235.087 kb on + strand at 4235.099 kb on + strand at 4235.162 kb on - strand at 4235.195 kb on + strand at 4235.227 kb on - strand at 4235.235 kb on + strand at 4235.238 kb on + strand at 4235.239 kb on - strand at 4235.302 kb on + strand at 4235.303 kb on - strand at 4235.303 kb on - strand at 4235.441 kb on + strand at 4235.484 kb on + strand at 4235.502 kb on + strand at 4235.535 kb on + strand at 4235.535 kb on + strand at 4235.535 kb on + strand at 4235.535 kb on + strand at 4235.684 kb on + strand at 4235.684 kb on + strand at 4235.707 kb on + strand at 4235.798 kb on - strand at 4235.815 kb on - strand at 4235.815 kb on - strand at 4235.815 kb on - strand at 4235.834 kb on - strand at 4235.841 kb on + strand at 4235.842 kb on - strand at 4235.885 kb on - strand, within BT3302 at 4235.898 kb on - strand, within BT3302 at 4235.903 kb on + strand, within BT3302 at 4235.903 kb on + strand, within BT3302 at 4235.904 kb on - strand, within BT3302 at 4235.906 kb on + strand, within BT3302 at 4235.907 kb on - strand, within BT3302 at 4235.907 kb on - strand, within BT3302 at 4235.907 kb on - strand, within BT3302 at 4235.990 kb on - strand, within BT3302 at 4236.038 kb on + strand, within BT3302 at 4236.038 kb on + strand, within BT3302 at 4236.041 kb on - strand, within BT3302 at 4236.041 kb on - strand, within BT3302 at 4236.043 kb on - strand, within BT3302
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 4,232,938 - BT3300 0.66 -0.9 4,232,940 + BT3300 0.66 -0.1 4,232,947 + BT3300 0.67 -0.4 4,232,954 - BT3300 0.67 +0.1 4,233,048 - -2.4 4,233,077 + BT3300 0.72 +1.1 4,233,112 - BT3300 0.73 -2.1 4,233,133 - BT3300 0.74 +1.2 4,233,133 - BT3300 0.74 -1.8 4,233,133 - BT3300 0.74 -0.2 4,233,178 + BT3300 0.76 -2.7 4,233,179 - BT3300 0.76 -2.7 4,233,205 - BT3300 0.77 +2.2 4,233,226 + BT3300 0.78 -0.0 4,233,232 + BT3300 0.78 +0.5 4,233,236 - BT3300 0.78 -0.8 4,233,302 + BT3300 0.81 +1.2 4,233,328 + BT3300 0.82 -1.4 4,233,329 - BT3300 0.82 +1.4 4,233,338 + BT3300 0.82 -1.8 4,233,339 - BT3300 0.82 -1.5 4,233,343 - BT3300 0.83 -0.8 4,233,359 - BT3300 0.83 +1.5 4,233,360 + BT3300 0.83 +1.8 4,233,360 + BT3300 0.83 -0.8 4,233,361 - BT3300 0.83 -1.1 4,233,361 - BT3300 0.83 -3.1 4,233,397 + BT3300 0.85 -0.3 4,233,398 - BT3300 0.85 +1.4 4,233,413 + BT3300 0.85 -2.0 4,233,493 + BT3300 0.89 +0.2 4,233,609 + +0.5 4,233,610 - -0.2 4,233,650 + -2.6 4,233,668 + +1.8 4,233,668 + +2.8 4,233,700 + -2.6 4,233,702 + +0.8 4,233,793 + -1.0 4,233,793 + -2.6 4,233,812 - +1.0 4,233,847 + +1.5 4,233,852 + -0.8 4,234,023 + BT3301 0.11 -0.8 4,234,028 + BT3301 0.12 -0.2 4,234,029 - BT3301 0.12 -2.8 4,234,097 + BT3301 0.18 -2.6 4,234,141 + BT3301 0.21 -0.9 4,234,182 + BT3301 0.25 +1.2 4,234,183 - BT3301 0.25 -1.9 4,234,183 - BT3301 0.25 -3.3 4,234,183 - BT3301 0.25 -0.2 4,234,307 + BT3301 0.35 -2.6 4,234,313 - BT3301 0.36 -1.2 4,234,352 - BT3301 0.39 +0.5 4,234,381 + BT3301 0.42 -3.3 4,234,429 + BT3301 0.46 +0.2 4,234,429 + BT3301 0.46 -1.0 4,234,448 + BT3301 0.47 -1.6 4,234,506 + BT3301 0.52 -0.4 4,234,528 + BT3301 0.54 -2.1 4,234,607 - BT3301 0.61 -0.2 4,234,633 + BT3301 0.63 +1.2 4,234,636 + BT3301 0.63 +1.6 4,234,693 + BT3301 0.68 -0.8 4,234,737 - BT3301 0.72 -3.0 4,234,737 - BT3301 0.72 -2.3 4,234,762 - BT3301 0.74 -1.6 4,234,784 + BT3301 0.76 -1.8 4,234,784 + BT3301 0.76 -2.4 4,234,818 + BT3301 0.78 -2.0 4,234,827 - BT3301 0.79 -1.3 4,234,831 - BT3301 0.79 -0.1 4,234,843 + BT3301 0.80 +0.2 4,234,885 + BT3301 0.84 +0.6 4,234,885 + BT3301 0.84 -2.3 4,234,951 - BT3301 0.90 -1.8 4,235,082 - -0.6 4,235,084 + -1.2 4,235,085 - +0.2 4,235,087 + -2.0 4,235,099 + -0.3 4,235,162 - -2.3 4,235,195 + -2.4 4,235,227 - +1.2 4,235,235 + -1.8 4,235,238 + -1.8 4,235,239 - +1.0 4,235,302 + -1.7 4,235,303 - +0.2 4,235,303 - +1.5 4,235,441 + -1.2 4,235,484 + +0.2 4,235,502 + +1.6 4,235,535 + +3.2 4,235,535 + +1.2 4,235,535 + +0.0 4,235,535 + +0.6 4,235,684 + -1.4 4,235,684 + -0.2 4,235,707 + -0.7 4,235,798 - +1.9 4,235,815 - -2.8 4,235,815 - -0.6 4,235,815 - +0.5 4,235,834 - +1.4 4,235,841 + -0.9 4,235,842 - -2.1 4,235,885 - BT3302 0.11 +2.8 4,235,898 - BT3302 0.11 -1.1 4,235,903 + BT3302 0.11 -2.4 4,235,903 + BT3302 0.11 +1.2 4,235,904 - BT3302 0.11 -0.9 4,235,906 + BT3302 0.11 -1.9 4,235,907 - BT3302 0.11 -1.1 4,235,907 - BT3302 0.11 +0.6 4,235,907 - BT3302 0.11 +2.5 4,235,990 - BT3302 0.13 -0.6 4,236,038 + BT3302 0.14 +1.2 4,236,038 + BT3302 0.14 +0.2 4,236,041 - BT3302 0.15 -3.3 4,236,041 - BT3302 0.15 -1.4 4,236,043 - BT3302 0.15 -0.7
Or see this region's nucleotide sequence