Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2385 and BT2386 are separated by 47 nucleotides BT2386 and BT2387 are separated by 178 nucleotides BT2387 and BT2388 are separated by 124 nucleotides BT2388 and BT2389 are separated by 99 nucleotides
BT2385: BT2385 - conserved hypothetical protein (NCBI ptt file), at 2,973,372 to 2,974,241
BT2385
BT2386: BT2386 - AsnC family transcriptional regulator (NCBI ptt file), at 2,974,289 to 2,974,759
BT2386
BT2387: BT2387 - O-acetylhomoserine (thiol)-lyase (NCBI ptt file), at 2,974,938 to 2,976,224
BT2387
BT2388: BT2388 - hypothetical protein (NCBI ptt file), at 2,976,349 to 2,976,588
BT2388
BT2389: BT2389 - putative transport protein (NCBI ptt file), at 2,976,688 to 2,977,599
BT2389
Position (kb)
2974
2975
2976
2977 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2973.978 kb on + strand, within BT2385 at 2973.981 kb on - strand, within BT2385 at 2974.011 kb on - strand, within BT2385 at 2974.018 kb on - strand, within BT2385 at 2974.019 kb on + strand, within BT2385 at 2974.020 kb on - strand, within BT2385 at 2974.067 kb on - strand, within BT2385 at 2974.067 kb on - strand, within BT2385 at 2974.067 kb on - strand, within BT2385 at 2974.068 kb on + strand, within BT2385 at 2974.068 kb on + strand, within BT2385 at 2974.084 kb on + strand, within BT2385 at 2974.217 kb on + strand at 2974.299 kb on - strand at 2974.399 kb on - strand, within BT2386 at 2974.408 kb on + strand, within BT2386 at 2974.431 kb on + strand, within BT2386 at 2974.450 kb on + strand, within BT2386 at 2974.450 kb on + strand, within BT2386 at 2974.451 kb on - strand, within BT2386 at 2974.453 kb on + strand, within BT2386 at 2974.454 kb on - strand, within BT2386 at 2974.484 kb on + strand, within BT2386 at 2974.529 kb on + strand, within BT2386 at 2974.591 kb on + strand, within BT2386 at 2974.601 kb on + strand, within BT2386 at 2974.601 kb on + strand, within BT2386 at 2974.601 kb on + strand, within BT2386 at 2974.603 kb on + strand, within BT2386 at 2974.603 kb on + strand, within BT2386 at 2974.604 kb on - strand, within BT2386 at 2974.606 kb on - strand, within BT2386 at 2974.627 kb on + strand, within BT2386 at 2974.718 kb on + strand at 2974.719 kb on - strand at 2974.741 kb on - strand at 2974.970 kb on - strand at 2974.970 kb on - strand at 2974.981 kb on + strand at 2975.027 kb on + strand at 2975.027 kb on + strand at 2975.027 kb on + strand at 2975.028 kb on - strand at 2975.031 kb on + strand at 2975.031 kb on + strand at 2975.046 kb on + strand at 2975.046 kb on + strand at 2975.051 kb on - strand at 2975.105 kb on + strand, within BT2387 at 2975.109 kb on + strand, within BT2387 at 2975.213 kb on + strand, within BT2387 at 2975.213 kb on + strand, within BT2387 at 2975.213 kb on + strand, within BT2387 at 2975.286 kb on + strand, within BT2387 at 2975.287 kb on - strand, within BT2387 at 2975.303 kb on + strand, within BT2387 at 2975.303 kb on + strand, within BT2387 at 2975.304 kb on - strand, within BT2387 at 2975.304 kb on - strand, within BT2387 at 2975.395 kb on + strand, within BT2387 at 2975.395 kb on + strand, within BT2387 at 2975.399 kb on + strand, within BT2387 at 2975.429 kb on + strand, within BT2387 at 2975.432 kb on + strand, within BT2387 at 2975.440 kb on + strand, within BT2387 at 2975.441 kb on - strand, within BT2387 at 2975.441 kb on - strand, within BT2387 at 2975.476 kb on + strand, within BT2387 at 2975.488 kb on + strand, within BT2387 at 2975.496 kb on - strand, within BT2387 at 2975.512 kb on - strand, within BT2387 at 2975.602 kb on + strand, within BT2387 at 2975.602 kb on + strand, within BT2387 at 2975.602 kb on + strand, within BT2387 at 2975.633 kb on - strand, within BT2387 at 2975.870 kb on + strand, within BT2387 at 2975.935 kb on - strand, within BT2387 at 2976.052 kb on + strand, within BT2387 at 2976.071 kb on - strand, within BT2387 at 2976.089 kb on + strand, within BT2387 at 2976.152 kb on - strand at 2976.235 kb on + strand at 2976.240 kb on + strand at 2976.314 kb on - strand at 2976.314 kb on - strand at 2976.314 kb on - strand at 2976.319 kb on - strand at 2976.320 kb on + strand at 2976.320 kb on + strand at 2976.320 kb on + strand at 2976.328 kb on + strand at 2976.328 kb on + strand at 2976.335 kb on - strand at 2976.359 kb on - strand at 2976.475 kb on + strand, within BT2388 at 2976.476 kb on - strand, within BT2388 at 2976.485 kb on + strand, within BT2388 at 2976.498 kb on + strand, within BT2388 at 2976.498 kb on + strand, within BT2388 at 2976.533 kb on + strand, within BT2388 at 2976.534 kb on - strand, within BT2388 at 2976.534 kb on - strand, within BT2388 at 2976.556 kb on + strand, within BT2388 at 2976.587 kb on - strand at 2976.669 kb on - strand at 2976.672 kb on - strand at 2976.673 kb on + strand at 2976.674 kb on - strand at 2976.674 kb on - strand at 2976.675 kb on + strand at 2976.675 kb on + strand at 2976.675 kb on + strand at 2976.676 kb on - strand at 2976.676 kb on - strand at 2976.680 kb on + strand at 2976.681 kb on - strand at 2976.885 kb on + strand, within BT2389 at 2976.991 kb on - strand, within BT2389 at 2976.996 kb on - strand, within BT2389 at 2977.138 kb on - strand, within BT2389
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 2,973,978 + BT2385 0.70 -1.9 2,973,981 - BT2385 0.70 -1.9 2,974,011 - BT2385 0.73 +2.1 2,974,018 - BT2385 0.74 +0.6 2,974,019 + BT2385 0.74 -2.0 2,974,020 - BT2385 0.74 -0.2 2,974,067 - BT2385 0.80 -0.9 2,974,067 - BT2385 0.80 +0.9 2,974,067 - BT2385 0.80 +0.1 2,974,068 + BT2385 0.80 -0.7 2,974,068 + BT2385 0.80 -2.2 2,974,084 + BT2385 0.82 -0.5 2,974,217 + -2.3 2,974,299 - +0.8 2,974,399 - BT2386 0.23 -0.2 2,974,408 + BT2386 0.25 +0.1 2,974,431 + BT2386 0.30 +2.9 2,974,450 + BT2386 0.34 -0.5 2,974,450 + BT2386 0.34 +0.1 2,974,451 - BT2386 0.34 -0.6 2,974,453 + BT2386 0.35 -0.6 2,974,454 - BT2386 0.35 -1.7 2,974,484 + BT2386 0.41 -0.1 2,974,529 + BT2386 0.51 +1.5 2,974,591 + BT2386 0.64 -1.4 2,974,601 + BT2386 0.66 +0.7 2,974,601 + BT2386 0.66 -1.7 2,974,601 + BT2386 0.66 +0.1 2,974,603 + BT2386 0.67 -0.6 2,974,603 + BT2386 0.67 -1.5 2,974,604 - BT2386 0.67 +1.8 2,974,606 - BT2386 0.67 -1.6 2,974,627 + BT2386 0.72 -2.1 2,974,718 + -0.5 2,974,719 - +0.7 2,974,741 - -0.7 2,974,970 - +0.1 2,974,970 - -0.9 2,974,981 + -1.9 2,975,027 + -2.2 2,975,027 + -1.9 2,975,027 + -2.2 2,975,028 - +1.4 2,975,031 + -0.9 2,975,031 + +0.8 2,975,046 + -1.0 2,975,046 + -0.3 2,975,051 - +3.1 2,975,105 + BT2387 0.13 -1.3 2,975,109 + BT2387 0.13 -1.6 2,975,213 + BT2387 0.21 +0.1 2,975,213 + BT2387 0.21 +0.7 2,975,213 + BT2387 0.21 -1.1 2,975,286 + BT2387 0.27 +1.1 2,975,287 - BT2387 0.27 -0.7 2,975,303 + BT2387 0.28 -0.8 2,975,303 + BT2387 0.28 -2.7 2,975,304 - BT2387 0.28 -1.5 2,975,304 - BT2387 0.28 -2.3 2,975,395 + BT2387 0.36 -2.5 2,975,395 + BT2387 0.36 +1.1 2,975,399 + BT2387 0.36 +3.3 2,975,429 + BT2387 0.38 +0.3 2,975,432 + BT2387 0.38 -2.2 2,975,440 + BT2387 0.39 -1.0 2,975,441 - BT2387 0.39 -0.9 2,975,441 - BT2387 0.39 -0.9 2,975,476 + BT2387 0.42 +2.7 2,975,488 + BT2387 0.43 +2.2 2,975,496 - BT2387 0.43 +2.1 2,975,512 - BT2387 0.45 +0.7 2,975,602 + BT2387 0.52 -1.9 2,975,602 + BT2387 0.52 +3.9 2,975,602 + BT2387 0.52 -1.7 2,975,633 - BT2387 0.54 +0.3 2,975,870 + BT2387 0.72 -0.5 2,975,935 - BT2387 0.77 -1.5 2,976,052 + BT2387 0.87 -1.3 2,976,071 - BT2387 0.88 +1.1 2,976,089 + BT2387 0.89 -0.7 2,976,152 - -0.1 2,976,235 + -1.5 2,976,240 + -0.5 2,976,314 - -1.2 2,976,314 - -1.2 2,976,314 - -1.5 2,976,319 - -0.6 2,976,320 + +0.1 2,976,320 + -2.2 2,976,320 + -0.6 2,976,328 + -1.5 2,976,328 + -0.5 2,976,335 - +2.4 2,976,359 - -1.4 2,976,475 + BT2388 0.53 -2.2 2,976,476 - BT2388 0.53 -4.2 2,976,485 + BT2388 0.57 -1.1 2,976,498 + BT2388 0.62 +1.1 2,976,498 + BT2388 0.62 -0.8 2,976,533 + BT2388 0.77 -1.7 2,976,534 - BT2388 0.77 -2.0 2,976,534 - BT2388 0.77 -1.9 2,976,556 + BT2388 0.86 -2.4 2,976,587 - -0.8 2,976,669 - -2.7 2,976,672 - +0.9 2,976,673 + -1.3 2,976,674 - -3.5 2,976,674 - -1.8 2,976,675 + +2.4 2,976,675 + -0.9 2,976,675 + +0.3 2,976,676 - -2.5 2,976,676 - -0.7 2,976,680 + -1.4 2,976,681 - +2.1 2,976,885 + BT2389 0.22 +0.1 2,976,991 - BT2389 0.33 -0.9 2,976,996 - BT2389 0.34 -2.2 2,977,138 - BT2389 0.49 -1.5
Or see this region's nucleotide sequence