Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2211 and BT2212 are separated by 59 nucleotides BT2212 and BT2213 are separated by 11 nucleotides
BT2211: BT2211 - conserved hypothetical protein (NCBI ptt file), at 2,778,788 to 2,780,098
BT2211
BT2212: BT2212 - magnesium chelatase, subunit I, putative ATPase (NCBI ptt file), at 2,780,158 to 2,781,132
BT2212
BT2213: BT2213 - hypothetical protein (NCBI ptt file), at 2,781,144 to 2,782,418
BT2213
Position (kb)
2780
2781
2782 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2779.174 kb on + strand, within BT2211 at 2779.188 kb on + strand, within BT2211 at 2779.213 kb on + strand, within BT2211 at 2779.214 kb on - strand, within BT2211 at 2779.328 kb on - strand, within BT2211 at 2779.389 kb on - strand, within BT2211 at 2779.433 kb on + strand, within BT2211 at 2779.433 kb on + strand, within BT2211 at 2779.496 kb on + strand, within BT2211 at 2779.501 kb on - strand, within BT2211 at 2779.547 kb on - strand, within BT2211 at 2779.573 kb on + strand, within BT2211 at 2779.574 kb on - strand, within BT2211 at 2779.623 kb on + strand, within BT2211 at 2779.746 kb on + strand, within BT2211 at 2779.746 kb on + strand, within BT2211 at 2779.813 kb on + strand, within BT2211 at 2779.821 kb on - strand, within BT2211 at 2779.957 kb on + strand, within BT2211 at 2779.958 kb on - strand, within BT2211 at 2779.958 kb on - strand, within BT2211 at 2779.958 kb on - strand, within BT2211 at 2779.959 kb on + strand, within BT2211 at 2779.960 kb on - strand, within BT2211 at 2779.960 kb on - strand, within BT2211 at 2780.048 kb on + strand at 2780.076 kb on + strand at 2780.077 kb on - strand at 2780.379 kb on - strand, within BT2212 at 2780.380 kb on + strand, within BT2212 at 2780.424 kb on - strand, within BT2212 at 2780.436 kb on + strand, within BT2212 at 2780.436 kb on + strand, within BT2212 at 2780.443 kb on + strand, within BT2212 at 2780.524 kb on + strand, within BT2212 at 2780.538 kb on + strand, within BT2212 at 2780.538 kb on + strand, within BT2212 at 2780.582 kb on + strand, within BT2212 at 2780.676 kb on + strand, within BT2212 at 2780.681 kb on + strand, within BT2212 at 2780.682 kb on - strand, within BT2212 at 2780.698 kb on - strand, within BT2212 at 2780.719 kb on + strand, within BT2212 at 2780.720 kb on - strand, within BT2212 at 2780.795 kb on - strand, within BT2212 at 2780.848 kb on - strand, within BT2212 at 2780.987 kb on - strand, within BT2212 at 2781.132 kb on + strand at 2781.132 kb on + strand at 2781.136 kb on + strand at 2781.146 kb on - strand at 2781.146 kb on - strand at 2781.180 kb on - strand at 2781.367 kb on + strand, within BT2213 at 2781.438 kb on - strand, within BT2213 at 2781.503 kb on + strand, within BT2213 at 2781.503 kb on + strand, within BT2213 at 2781.541 kb on + strand, within BT2213 at 2781.542 kb on - strand, within BT2213 at 2781.617 kb on + strand, within BT2213 at 2781.617 kb on + strand, within BT2213 at 2781.619 kb on + strand at 2781.676 kb on + strand, within BT2213 at 2781.787 kb on + strand, within BT2213 at 2781.788 kb on - strand, within BT2213 at 2781.788 kb on - strand, within BT2213 at 2781.968 kb on - strand, within BT2213 at 2782.034 kb on - strand, within BT2213 at 2782.055 kb on + strand, within BT2213 at 2782.055 kb on + strand, within BT2213 at 2782.100 kb on + strand, within BT2213 at 2782.100 kb on + strand, within BT2213 at 2782.101 kb on - strand, within BT2213 at 2782.101 kb on - strand, within BT2213 at 2782.101 kb on - strand, within BT2213
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 2,779,174 + BT2211 0.29 +0.8 2,779,188 + BT2211 0.31 -3.2 2,779,213 + BT2211 0.32 -2.5 2,779,214 - BT2211 0.32 +0.6 2,779,328 - BT2211 0.41 -1.5 2,779,389 - BT2211 0.46 +2.1 2,779,433 + BT2211 0.49 -2.7 2,779,433 + BT2211 0.49 +0.4 2,779,496 + BT2211 0.54 -0.6 2,779,501 - BT2211 0.54 -1.8 2,779,547 - BT2211 0.58 +0.8 2,779,573 + BT2211 0.60 +0.1 2,779,574 - BT2211 0.60 -1.0 2,779,623 + BT2211 0.64 -1.6 2,779,746 + BT2211 0.73 -2.2 2,779,746 + BT2211 0.73 +1.7 2,779,813 + BT2211 0.78 +1.1 2,779,821 - BT2211 0.79 -2.2 2,779,957 + BT2211 0.89 -0.9 2,779,958 - BT2211 0.89 +0.4 2,779,958 - BT2211 0.89 +1.1 2,779,958 - BT2211 0.89 -1.7 2,779,959 + BT2211 0.89 -0.8 2,779,960 - BT2211 0.89 +1.1 2,779,960 - BT2211 0.89 +0.1 2,780,048 + -0.9 2,780,076 + +1.7 2,780,077 - -3.2 2,780,379 - BT2212 0.23 +0.7 2,780,380 + BT2212 0.23 -1.6 2,780,424 - BT2212 0.27 +1.1 2,780,436 + BT2212 0.29 +1.4 2,780,436 + BT2212 0.29 -0.7 2,780,443 + BT2212 0.29 -0.0 2,780,524 + BT2212 0.38 +0.1 2,780,538 + BT2212 0.39 -0.1 2,780,538 + BT2212 0.39 +1.9 2,780,582 + BT2212 0.43 -1.5 2,780,676 + BT2212 0.53 +3.2 2,780,681 + BT2212 0.54 -1.5 2,780,682 - BT2212 0.54 +2.4 2,780,698 - BT2212 0.55 -0.9 2,780,719 + BT2212 0.58 +0.1 2,780,720 - BT2212 0.58 -2.1 2,780,795 - BT2212 0.65 +1.1 2,780,848 - BT2212 0.71 +3.0 2,780,987 - BT2212 0.85 -0.6 2,781,132 + +1.7 2,781,132 + +0.9 2,781,136 + -0.6 2,781,146 - -1.8 2,781,146 - +1.7 2,781,180 - +0.7 2,781,367 + BT2213 0.17 -1.5 2,781,438 - BT2213 0.23 -0.5 2,781,503 + BT2213 0.28 -2.0 2,781,503 + BT2213 0.28 -3.4 2,781,541 + BT2213 0.31 -4.5 2,781,542 - BT2213 0.31 -0.2 2,781,617 + BT2213 0.37 -3.1 2,781,617 + BT2213 0.37 +0.7 2,781,619 + +0.7 2,781,676 + BT2213 0.42 -1.1 2,781,787 + BT2213 0.50 +0.1 2,781,788 - BT2213 0.51 -1.4 2,781,788 - BT2213 0.51 -1.6 2,781,968 - BT2213 0.65 -1.1 2,782,034 - BT2213 0.70 +0.1 2,782,055 + BT2213 0.71 -1.4 2,782,055 + BT2213 0.71 +1.1 2,782,100 + BT2213 0.75 -0.9 2,782,100 + BT2213 0.75 -1.2 2,782,101 - BT2213 0.75 -0.8 2,782,101 - BT2213 0.75 +3.8 2,782,101 - BT2213 0.75 -1.1
Or see this region's nucleotide sequence