Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT2177

Experiment: BHIS with Trimethoprim 0.00625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT2174 and BT2175 overlap by 22 nucleotidesBT2175 and BT2176 are separated by 143 nucleotidesBT2176 and BT2177 overlap by 4 nucleotidesBT2177 and BT2178 are separated by 292 nucleotidesBT2178 and BT2179 are separated by 129 nucleotides BT2174: BT2174 - ABC transporter ATP-binding protein (NCBI ptt file), at 2,734,946 to 2,735,623 BT2174 BT2175: BT2175 - L-fuculose kinase FucK (EC 2.7.1.51); D-ribulose 1-kinase (from data), at 2,735,602 to 2,735,958 BT2175 BT2176: BT2176 - hypothetical protein (NCBI ptt file), at 2,736,102 to 2,736,389 BT2176 BT2177: BT2177 - putative membrane protein (NCBI ptt file), at 2,736,386 to 2,736,994 BT2177 BT2178: BT2178 - hypothetical protein (NCBI ptt file), at 2,737,287 to 2,737,643 BT2178 BT2179: BT2179 - putative DNA mismatch repair protein (NCBI ptt file), at 2,737,773 to 2,738,837 BT2179 Position (kb) 2736 2737Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 2735.387 kb on + strand, within BT2174at 2735.425 kb on + strand, within BT2174at 2735.425 kb on + strand, within BT2174at 2735.426 kb on - strand, within BT2174at 2735.528 kb on - strand, within BT2174at 2735.540 kb on - strand, within BT2174at 2735.596 kb on + strandat 2735.597 kb on - strandat 2735.597 kb on - strandat 2735.597 kb on - strandat 2735.641 kb on + strand, within BT2175at 2735.652 kb on - strand, within BT2175at 2735.653 kb on + strand, within BT2175at 2735.654 kb on - strand, within BT2175at 2735.714 kb on + strand, within BT2175at 2735.715 kb on - strand, within BT2175at 2735.739 kb on + strand, within BT2175at 2735.741 kb on + strand, within BT2175at 2735.776 kb on + strand, within BT2175at 2735.836 kb on + strand, within BT2175at 2735.836 kb on + strand, within BT2175at 2735.837 kb on - strand, within BT2175at 2735.837 kb on - strand, within BT2175at 2735.837 kb on - strand, within BT2175at 2735.841 kb on + strand, within BT2175at 2735.907 kb on + strand, within BT2175at 2735.914 kb on + strand, within BT2175at 2735.914 kb on + strand, within BT2175at 2735.914 kb on + strand, within BT2175at 2736.045 kb on + strandat 2736.063 kb on + strandat 2736.064 kb on - strandat 2736.098 kb on + strandat 2736.098 kb on + strandat 2736.098 kb on + strandat 2736.099 kb on - strandat 2736.122 kb on + strandat 2736.123 kb on - strandat 2736.178 kb on + strand, within BT2176at 2736.182 kb on + strand, within BT2176at 2736.183 kb on - strand, within BT2176at 2736.236 kb on - strand, within BT2176at 2736.251 kb on + strand, within BT2176at 2736.321 kb on - strand, within BT2176at 2736.323 kb on - strand, within BT2176at 2736.349 kb on + strand, within BT2176at 2736.349 kb on + strand, within BT2176at 2736.350 kb on - strand, within BT2176at 2736.352 kb on + strand, within BT2176at 2736.353 kb on - strand, within BT2176at 2736.354 kb on + strand, within BT2176at 2736.354 kb on + strand, within BT2176at 2736.354 kb on + strand, within BT2176at 2736.354 kb on + strand, within BT2176at 2736.358 kb on + strand, within BT2176at 2736.365 kb on + strandat 2736.386 kb on - strandat 2736.444 kb on - strandat 2736.481 kb on - strand, within BT2177at 2736.485 kb on + strand, within BT2177at 2736.486 kb on - strand, within BT2177at 2736.486 kb on - strand, within BT2177at 2736.520 kb on + strand, within BT2177at 2736.521 kb on - strand, within BT2177at 2736.631 kb on - strand, within BT2177at 2736.712 kb on + strand, within BT2177at 2736.714 kb on + strand, within BT2177at 2736.715 kb on - strand, within BT2177at 2736.730 kb on + strand, within BT2177at 2736.787 kb on - strand, within BT2177at 2736.838 kb on + strand, within BT2177at 2736.872 kb on - strand, within BT2177at 2737.230 kb on + strandat 2737.252 kb on + strandat 2737.265 kb on + strandat 2737.284 kb on - strandat 2737.284 kb on - strandat 2737.286 kb on + strandat 2737.361 kb on + strand, within BT2178at 2737.397 kb on + strand, within BT2178at 2737.421 kb on - strand, within BT2178at 2737.506 kb on - strand, within BT2178at 2737.534 kb on - strand, within BT2178at 2737.587 kb on + strand, within BT2178at 2737.588 kb on - strand, within BT2178at 2737.608 kb on - strandat 2737.608 kb on - strandat 2737.612 kb on + strandat 2737.638 kb on - strandat 2737.641 kb on + strandat 2737.655 kb on - strandat 2737.655 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM
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2,735,387 + BT2174 0.65 -0.6
2,735,425 + BT2174 0.71 -2.0
2,735,425 + BT2174 0.71 -2.7
2,735,426 - BT2174 0.71 -1.9
2,735,528 - BT2174 0.86 -0.3
2,735,540 - BT2174 0.88 -2.5
2,735,596 + +1.4
2,735,597 - +0.1
2,735,597 - -1.5
2,735,597 - -0.3
2,735,641 + BT2175 0.11 +1.1
2,735,652 - BT2175 0.14 -2.0
2,735,653 + BT2175 0.14 -2.5
2,735,654 - BT2175 0.15 -0.9
2,735,714 + BT2175 0.31 -2.4
2,735,715 - BT2175 0.32 -1.8
2,735,739 + BT2175 0.38 +0.1
2,735,741 + BT2175 0.39 -1.9
2,735,776 + BT2175 0.49 -2.2
2,735,836 + BT2175 0.66 +0.1
2,735,836 + BT2175 0.66 +3.3
2,735,837 - BT2175 0.66 -1.5
2,735,837 - BT2175 0.66 -0.9
2,735,837 - BT2175 0.66 -0.5
2,735,841 + BT2175 0.67 -3.2
2,735,907 + BT2175 0.85 +0.7
2,735,914 + BT2175 0.87 +0.2
2,735,914 + BT2175 0.87 -1.5
2,735,914 + BT2175 0.87 -0.9
2,736,045 + -0.3
2,736,063 + +2.1
2,736,064 - +0.5
2,736,098 + +1.7
2,736,098 + +1.3
2,736,098 + +0.7
2,736,099 - -1.9
2,736,122 + +1.4
2,736,123 - -3.1
2,736,178 + BT2176 0.26 -1.2
2,736,182 + BT2176 0.28 -2.3
2,736,183 - BT2176 0.28 -0.8
2,736,236 - BT2176 0.47 -1.1
2,736,251 + BT2176 0.52 +1.9
2,736,321 - BT2176 0.76 -1.2
2,736,323 - BT2176 0.77 -0.3
2,736,349 + BT2176 0.86 +0.1
2,736,349 + BT2176 0.86 +2.1
2,736,350 - BT2176 0.86 -0.3
2,736,352 + BT2176 0.87 -1.9
2,736,353 - BT2176 0.87 -1.1
2,736,354 + BT2176 0.88 -2.6
2,736,354 + BT2176 0.88 -0.5
2,736,354 + BT2176 0.88 -4.1
2,736,354 + BT2176 0.88 -1.1
2,736,358 + BT2176 0.89 +1.1
2,736,365 + -3.8
2,736,386 - -0.2
2,736,444 - -1.7
2,736,481 - BT2177 0.16 +3.6
2,736,485 + BT2177 0.16 +1.1
2,736,486 - BT2177 0.16 +1.7
2,736,486 - BT2177 0.16 +0.1
2,736,520 + BT2177 0.22 -0.1
2,736,521 - BT2177 0.22 -0.3
2,736,631 - BT2177 0.40 -3.5
2,736,712 + BT2177 0.54 +0.4
2,736,714 + BT2177 0.54 +2.3
2,736,715 - BT2177 0.54 -1.9
2,736,730 + BT2177 0.56 -1.7
2,736,787 - BT2177 0.66 -1.7
2,736,838 + BT2177 0.74 +2.1
2,736,872 - BT2177 0.80 -0.6
2,737,230 + -2.9
2,737,252 + +1.4
2,737,265 + -1.5
2,737,284 - +2.4
2,737,284 - -1.2
2,737,286 + -1.9
2,737,361 + BT2178 0.21 -1.2
2,737,397 + BT2178 0.31 +0.1
2,737,421 - BT2178 0.38 -0.9
2,737,506 - BT2178 0.61 -3.1
2,737,534 - BT2178 0.69 -1.8
2,737,587 + BT2178 0.84 -0.7
2,737,588 - BT2178 0.84 -0.4
2,737,608 - +0.1
2,737,608 - +2.2
2,737,612 + -3.3
2,737,638 - -3.2
2,737,641 + +1.4
2,737,655 - +1.1
2,737,655 - +0.1

Or see this region's nucleotide sequence