Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT_2070 and BT2071 are separated by 64 nucleotides BT2071 and BT2072 are separated by 14 nucleotides
BT_2070: BT_2070 - methylcitrate synthase (RefSeq), at 2,591,718 to 2,593,022
_2070
BT2071: BT2071 - isocitrate dehydrogenase [NADP] (NCBI ptt file), at 2,593,087 to 2,594,277
BT2071
BT2072: BT2072 - aconitate hydratase (NCBI ptt file), at 2,594,292 to 2,596,535
BT2072
Position (kb)
2593
2594
2595 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2592.103 kb on + strand, within BT_2070 at 2592.119 kb on + strand, within BT_2070 at 2592.119 kb on + strand, within BT_2070 at 2592.124 kb on + strand, within BT_2070 at 2592.154 kb on + strand, within BT_2070 at 2592.155 kb on - strand, within BT_2070 at 2592.155 kb on - strand, within BT_2070 at 2592.155 kb on - strand, within BT_2070 at 2592.158 kb on + strand, within BT_2070 at 2592.159 kb on - strand, within BT_2070 at 2592.162 kb on + strand, within BT_2070 at 2592.192 kb on - strand, within BT_2070 at 2592.216 kb on - strand, within BT_2070 at 2592.289 kb on + strand, within BT_2070 at 2592.289 kb on + strand, within BT_2070 at 2592.289 kb on + strand, within BT_2070 at 2592.290 kb on - strand, within BT_2070 at 2592.293 kb on + strand, within BT_2070 at 2592.294 kb on - strand, within BT_2070 at 2592.294 kb on - strand, within BT_2070 at 2592.422 kb on + strand, within BT_2070 at 2592.423 kb on - strand, within BT_2070 at 2592.509 kb on + strand, within BT_2070 at 2592.510 kb on - strand, within BT_2070 at 2592.512 kb on - strand, within BT_2070 at 2592.545 kb on + strand, within BT_2070 at 2592.545 kb on + strand, within BT_2070 at 2592.585 kb on - strand, within BT_2070 at 2592.638 kb on + strand, within BT_2070 at 2592.701 kb on + strand, within BT_2070 at 2592.702 kb on - strand, within BT_2070 at 2592.890 kb on + strand, within BT_2070 at 2592.903 kb on + strand at 2592.903 kb on + strand at 2592.936 kb on + strand at 2592.937 kb on - strand at 2593.048 kb on + strand at 2593.048 kb on + strand at 2593.048 kb on + strand at 2593.065 kb on - strand at 2593.065 kb on - strand at 2593.075 kb on + strand at 2593.131 kb on + strand at 2593.143 kb on + strand at 2593.144 kb on - strand at 2593.247 kb on + strand, within BT2071 at 2593.368 kb on + strand, within BT2071 at 2593.368 kb on + strand, within BT2071 at 2593.369 kb on - strand, within BT2071 at 2593.428 kb on - strand, within BT2071 at 2593.463 kb on + strand, within BT2071 at 2593.486 kb on - strand, within BT2071 at 2593.784 kb on + strand, within BT2071 at 2593.785 kb on - strand, within BT2071 at 2593.832 kb on + strand, within BT2071 at 2593.832 kb on + strand, within BT2071 at 2593.832 kb on + strand, within BT2071 at 2593.833 kb on - strand, within BT2071 at 2593.833 kb on - strand, within BT2071 at 2593.834 kb on + strand, within BT2071 at 2593.835 kb on - strand, within BT2071 at 2593.835 kb on - strand, within BT2071 at 2593.835 kb on - strand, within BT2071 at 2593.835 kb on - strand, within BT2071 at 2593.837 kb on - strand, within BT2071 at 2593.837 kb on - strand, within BT2071 at 2593.837 kb on - strand, within BT2071 at 2593.846 kb on - strand, within BT2071 at 2594.211 kb on + strand at 2594.213 kb on + strand at 2594.213 kb on + strand at 2594.213 kb on + strand at 2594.214 kb on - strand at 2594.248 kb on - strand at 2594.248 kb on - strand at 2594.266 kb on + strand at 2594.347 kb on + strand at 2594.348 kb on - strand at 2594.351 kb on + strand at 2594.351 kb on + strand at 2594.351 kb on + strand at 2594.352 kb on - strand at 2594.499 kb on - strand at 2594.702 kb on - strand, within BT2072 at 2594.766 kb on + strand, within BT2072 at 2594.772 kb on - strand, within BT2072 at 2594.843 kb on + strand, within BT2072 at 2594.877 kb on - strand, within BT2072 at 2594.883 kb on + strand, within BT2072 at 2594.885 kb on + strand, within BT2072 at 2594.981 kb on + strand, within BT2072 at 2595.239 kb on + strand, within BT2072
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 2,592,103 + BT_2070 0.30 -2.2 2,592,119 + BT_2070 0.31 -1.9 2,592,119 + BT_2070 0.31 +2.4 2,592,124 + BT_2070 0.31 +1.8 2,592,154 + BT_2070 0.33 +1.5 2,592,155 - BT_2070 0.33 +0.7 2,592,155 - BT_2070 0.33 -1.3 2,592,155 - BT_2070 0.33 -1.1 2,592,158 + BT_2070 0.34 +2.0 2,592,159 - BT_2070 0.34 -2.8 2,592,162 + BT_2070 0.34 -1.1 2,592,192 - BT_2070 0.36 -3.2 2,592,216 - BT_2070 0.38 +0.6 2,592,289 + BT_2070 0.44 -0.5 2,592,289 + BT_2070 0.44 -0.3 2,592,289 + BT_2070 0.44 -0.9 2,592,290 - BT_2070 0.44 +1.7 2,592,293 + BT_2070 0.44 -1.3 2,592,294 - BT_2070 0.44 +1.1 2,592,294 - BT_2070 0.44 -0.9 2,592,422 + BT_2070 0.54 +1.8 2,592,423 - BT_2070 0.54 +0.3 2,592,509 + BT_2070 0.61 +0.1 2,592,510 - BT_2070 0.61 -0.7 2,592,512 - BT_2070 0.61 +1.2 2,592,545 + BT_2070 0.63 -1.9 2,592,545 + BT_2070 0.63 +0.6 2,592,585 - BT_2070 0.66 +0.4 2,592,638 + BT_2070 0.70 -1.0 2,592,701 + BT_2070 0.75 +1.7 2,592,702 - BT_2070 0.75 -2.8 2,592,890 + BT_2070 0.90 -0.9 2,592,903 + +1.7 2,592,903 + -1.1 2,592,936 + -1.9 2,592,937 - -1.2 2,593,048 + -3.7 2,593,048 + -0.9 2,593,048 + +1.3 2,593,065 - -1.9 2,593,065 - -1.7 2,593,075 + -0.2 2,593,131 + -1.7 2,593,143 + -2.1 2,593,144 - -1.1 2,593,247 + BT2071 0.13 +0.1 2,593,368 + BT2071 0.24 +0.1 2,593,368 + BT2071 0.24 -0.6 2,593,369 - BT2071 0.24 -0.3 2,593,428 - BT2071 0.29 -0.9 2,593,463 + BT2071 0.32 -0.9 2,593,486 - BT2071 0.34 -3.2 2,593,784 + BT2071 0.59 -2.5 2,593,785 - BT2071 0.59 -0.9 2,593,832 + BT2071 0.63 -2.7 2,593,832 + BT2071 0.63 -0.9 2,593,832 + BT2071 0.63 -0.8 2,593,833 - BT2071 0.63 -1.5 2,593,833 - BT2071 0.63 +1.9 2,593,834 + BT2071 0.63 -0.9 2,593,835 - BT2071 0.63 +0.1 2,593,835 - BT2071 0.63 +1.6 2,593,835 - BT2071 0.63 +1.1 2,593,835 - BT2071 0.63 -1.2 2,593,837 - BT2071 0.63 -1.6 2,593,837 - BT2071 0.63 +1.8 2,593,837 - BT2071 0.63 -2.7 2,593,846 - BT2071 0.64 -0.9 2,594,211 + +1.1 2,594,213 + -3.1 2,594,213 + -1.4 2,594,213 + -3.2 2,594,214 - -2.4 2,594,248 - -0.4 2,594,248 - -0.2 2,594,266 + -1.0 2,594,347 + -0.5 2,594,348 - +1.4 2,594,351 + +1.1 2,594,351 + +0.7 2,594,351 + -0.9 2,594,352 - +1.1 2,594,499 - -0.1 2,594,702 - BT2072 0.18 -0.6 2,594,766 + BT2072 0.21 -2.2 2,594,772 - BT2072 0.21 -1.9 2,594,843 + BT2072 0.25 -1.6 2,594,877 - BT2072 0.26 +2.3 2,594,883 + BT2072 0.26 -3.1 2,594,885 + BT2072 0.26 -0.9 2,594,981 + BT2072 0.31 -1.0 2,595,239 + BT2072 0.42 +3.1
Or see this region's nucleotide sequence