Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1809 and BT1810 are separated by 8 nucleotides BT1810 and BT1811 are separated by 112 nucleotides BT1811 and BT1812 are separated by 5 nucleotides
BT1809: BT1809 - conserved protein, with weak BamHI domain (NCBI ptt file), at 2,267,883 to 2,272,913
BT1809
BT1810: BT1810 - GAF domain-containing protein, involved in signal transduction (NCBI ptt file), at 2,272,922 to 2,273,392
BT1810
BT1811: BT1811 - hypothetical protein (NCBI ptt file), at 2,273,505 to 2,273,828
BT1811
BT1812: BT1812 - putative chaperone DnAJ (NCBI ptt file), at 2,273,834 to 2,274,799
BT1812
Position (kb)
2273
2274 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2272.541 kb on - strand at 2272.694 kb on + strand at 2272.951 kb on + strand at 2272.990 kb on - strand, within BT1810 at 2273.001 kb on + strand, within BT1810 at 2273.039 kb on - strand, within BT1810 at 2273.088 kb on - strand, within BT1810 at 2273.239 kb on - strand, within BT1810 at 2273.271 kb on + strand, within BT1810 at 2273.271 kb on + strand, within BT1810 at 2273.272 kb on - strand, within BT1810 at 2273.272 kb on - strand, within BT1810 at 2273.274 kb on + strand, within BT1810 at 2273.274 kb on + strand, within BT1810 at 2273.281 kb on + strand, within BT1810 at 2273.283 kb on + strand, within BT1810 at 2273.284 kb on - strand, within BT1810 at 2273.316 kb on + strand, within BT1810 at 2273.316 kb on + strand, within BT1810 at 2273.381 kb on + strand at 2273.402 kb on + strand at 2273.467 kb on + strand at 2273.524 kb on - strand at 2273.582 kb on + strand, within BT1811 at 2273.603 kb on + strand, within BT1811 at 2273.604 kb on - strand, within BT1811 at 2273.648 kb on - strand, within BT1811 at 2273.651 kb on - strand, within BT1811 at 2273.651 kb on - strand, within BT1811 at 2273.698 kb on + strand, within BT1811 at 2273.698 kb on + strand, within BT1811 at 2273.722 kb on - strand, within BT1811 at 2273.727 kb on - strand, within BT1811 at 2273.820 kb on - strand at 2273.911 kb on + strand at 2273.945 kb on - strand, within BT1812 at 2273.976 kb on - strand, within BT1812 at 2274.067 kb on + strand, within BT1812 at 2274.069 kb on + strand, within BT1812 at 2274.070 kb on - strand, within BT1812 at 2274.078 kb on - strand, within BT1812 at 2274.089 kb on + strand, within BT1812 at 2274.093 kb on + strand, within BT1812 at 2274.119 kb on + strand, within BT1812 at 2274.150 kb on + strand, within BT1812 at 2274.150 kb on + strand, within BT1812 at 2274.257 kb on - strand, within BT1812 at 2274.456 kb on + strand, within BT1812 at 2274.604 kb on - strand, within BT1812 at 2274.685 kb on + strand, within BT1812 at 2274.737 kb on + strand at 2274.738 kb on - strand at 2274.780 kb on + strand at 2274.781 kb on - strand at 2274.793 kb on - strand at 2274.793 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 2,272,541 - -2.5 2,272,694 + +2.7 2,272,951 + -0.6 2,272,990 - BT1810 0.14 -1.5 2,273,001 + BT1810 0.17 +2.1 2,273,039 - BT1810 0.25 -1.0 2,273,088 - BT1810 0.35 +4.2 2,273,239 - BT1810 0.67 +1.8 2,273,271 + BT1810 0.74 -0.5 2,273,271 + BT1810 0.74 -1.2 2,273,272 - BT1810 0.74 -1.6 2,273,272 - BT1810 0.74 +1.1 2,273,274 + BT1810 0.75 -1.1 2,273,274 + BT1810 0.75 -0.5 2,273,281 + BT1810 0.76 +0.1 2,273,283 + BT1810 0.77 -1.9 2,273,284 - BT1810 0.77 -1.3 2,273,316 + BT1810 0.84 -2.7 2,273,316 + BT1810 0.84 -2.8 2,273,381 + -0.6 2,273,402 + -1.5 2,273,467 + -0.5 2,273,524 - -1.1 2,273,582 + BT1811 0.24 -1.5 2,273,603 + BT1811 0.30 -1.5 2,273,604 - BT1811 0.31 -0.9 2,273,648 - BT1811 0.44 +0.4 2,273,651 - BT1811 0.45 +3.1 2,273,651 - BT1811 0.45 +0.3 2,273,698 + BT1811 0.60 -1.9 2,273,698 + BT1811 0.60 -0.9 2,273,722 - BT1811 0.67 +2.6 2,273,727 - BT1811 0.69 +4.1 2,273,820 - -0.7 2,273,911 + +2.9 2,273,945 - BT1812 0.11 -2.8 2,273,976 - BT1812 0.15 -2.5 2,274,067 + BT1812 0.24 -0.9 2,274,069 + BT1812 0.24 -1.9 2,274,070 - BT1812 0.24 -3.5 2,274,078 - BT1812 0.25 +2.7 2,274,089 + BT1812 0.26 -1.1 2,274,093 + BT1812 0.27 +0.7 2,274,119 + BT1812 0.30 -0.4 2,274,150 + BT1812 0.33 -1.7 2,274,150 + BT1812 0.33 -2.7 2,274,257 - BT1812 0.44 -1.5 2,274,456 + BT1812 0.64 -0.5 2,274,604 - BT1812 0.80 -0.4 2,274,685 + BT1812 0.88 -2.5 2,274,737 + -0.7 2,274,738 - +0.1 2,274,780 + -2.7 2,274,781 - +0.7 2,274,793 - -1.2 2,274,793 - -2.7
Or see this region's nucleotide sequence