Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1781 and BT1782 are separated by 940 nucleotides BT1782 and BT1783 overlap by 23 nucleotides
BT1781: BT1781 - xylosidase/arabinosidase (NCBI ptt file), at 2,224,974 to 2,227,013
BT1781
BT1782: BT1782 - putative phosphoesterase (NCBI ptt file), at 2,227,954 to 2,228,799
BT1782
BT1783: BT1783 - cation/H+ antiporter (NCBI ptt file), at 2,228,777 to 2,231,002
BT1783
Position (kb)
2227
2228
2229 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2226.986 kb on + strand at 2227.062 kb on + strand at 2227.071 kb on - strand at 2227.090 kb on + strand at 2227.090 kb on + strand at 2227.147 kb on + strand at 2227.152 kb on - strand at 2227.221 kb on + strand at 2227.253 kb on + strand at 2227.253 kb on + strand at 2227.276 kb on + strand at 2227.299 kb on + strand at 2227.372 kb on + strand at 2227.377 kb on - strand at 2227.492 kb on + strand at 2227.578 kb on + strand at 2227.579 kb on - strand at 2227.582 kb on - strand at 2227.607 kb on - strand at 2227.720 kb on + strand at 2227.908 kb on + strand at 2227.917 kb on + strand at 2227.947 kb on + strand at 2227.948 kb on - strand at 2228.018 kb on - strand at 2228.120 kb on + strand, within BT1782 at 2228.187 kb on + strand, within BT1782 at 2228.188 kb on - strand, within BT1782 at 2228.230 kb on - strand, within BT1782 at 2228.233 kb on + strand, within BT1782 at 2228.234 kb on - strand, within BT1782 at 2228.253 kb on - strand, within BT1782 at 2228.265 kb on + strand, within BT1782 at 2228.266 kb on - strand, within BT1782 at 2228.284 kb on - strand, within BT1782 at 2228.290 kb on + strand, within BT1782 at 2228.290 kb on + strand, within BT1782 at 2228.291 kb on - strand, within BT1782 at 2228.291 kb on - strand, within BT1782 at 2228.291 kb on - strand, within BT1782 at 2228.373 kb on + strand, within BT1782 at 2228.412 kb on - strand, within BT1782 at 2228.479 kb on - strand, within BT1782 at 2228.484 kb on - strand, within BT1782 at 2228.489 kb on + strand, within BT1782 at 2228.489 kb on + strand, within BT1782 at 2228.525 kb on - strand, within BT1782 at 2228.544 kb on + strand, within BT1782 at 2228.545 kb on - strand, within BT1782 at 2228.575 kb on - strand, within BT1782 at 2228.576 kb on + strand, within BT1782 at 2228.577 kb on - strand, within BT1782 at 2228.577 kb on - strand, within BT1782 at 2228.583 kb on - strand, within BT1782 at 2228.605 kb on - strand, within BT1782 at 2228.639 kb on + strand, within BT1782 at 2228.682 kb on - strand, within BT1782 at 2228.695 kb on - strand, within BT1782 at 2228.707 kb on + strand, within BT1782 at 2228.712 kb on - strand, within BT1782 at 2228.735 kb on - strand at 2228.762 kb on - strand at 2228.762 kb on - strand at 2228.764 kb on + strand at 2228.765 kb on - strand at 2228.812 kb on + strand at 2228.885 kb on - strand at 2228.886 kb on + strand at 2228.967 kb on - strand at 2228.980 kb on - strand at 2229.072 kb on - strand, within BT1783 at 2229.073 kb on + strand, within BT1783 at 2229.073 kb on + strand, within BT1783 at 2229.090 kb on - strand, within BT1783 at 2229.096 kb on - strand, within BT1783 at 2229.148 kb on + strand, within BT1783 at 2229.149 kb on - strand, within BT1783 at 2229.227 kb on + strand, within BT1783 at 2229.232 kb on + strand, within BT1783 at 2229.233 kb on - strand, within BT1783 at 2229.233 kb on - strand, within BT1783 at 2229.333 kb on - strand, within BT1783 at 2229.463 kb on + strand, within BT1783 at 2229.463 kb on + strand, within BT1783 at 2229.464 kb on - strand, within BT1783 at 2229.464 kb on - strand, within BT1783 at 2229.464 kb on - strand, within BT1783 at 2229.465 kb on + strand, within BT1783 at 2229.465 kb on + strand, within BT1783 at 2229.466 kb on - strand, within BT1783 at 2229.646 kb on + strand, within BT1783 at 2229.668 kb on - strand, within BT1783 at 2229.782 kb on - strand, within BT1783
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 2,226,986 + +3.1 2,227,062 + +0.8 2,227,071 - -1.3 2,227,090 + -0.3 2,227,090 + -0.2 2,227,147 + -1.7 2,227,152 - +0.8 2,227,221 + +1.7 2,227,253 + -0.5 2,227,253 + +0.1 2,227,276 + +0.8 2,227,299 + -2.6 2,227,372 + +0.8 2,227,377 - -1.5 2,227,492 + -0.5 2,227,578 + -1.2 2,227,579 - +2.2 2,227,582 - +1.8 2,227,607 - -1.6 2,227,720 + -2.1 2,227,908 + +0.5 2,227,917 + +1.1 2,227,947 + +0.1 2,227,948 - +0.1 2,228,018 - -1.5 2,228,120 + BT1782 0.20 +0.7 2,228,187 + BT1782 0.28 +1.1 2,228,188 - BT1782 0.28 -2.1 2,228,230 - BT1782 0.33 +4.3 2,228,233 + BT1782 0.33 -0.8 2,228,234 - BT1782 0.33 -1.5 2,228,253 - BT1782 0.35 -0.9 2,228,265 + BT1782 0.37 -1.9 2,228,266 - BT1782 0.37 -0.6 2,228,284 - BT1782 0.39 +0.1 2,228,290 + BT1782 0.40 +2.1 2,228,290 + BT1782 0.40 +2.3 2,228,291 - BT1782 0.40 +1.1 2,228,291 - BT1782 0.40 -0.4 2,228,291 - BT1782 0.40 +1.4 2,228,373 + BT1782 0.50 -1.9 2,228,412 - BT1782 0.54 +0.7 2,228,479 - BT1782 0.62 +2.3 2,228,484 - BT1782 0.63 +0.1 2,228,489 + BT1782 0.63 +1.1 2,228,489 + BT1782 0.63 -1.9 2,228,525 - BT1782 0.67 -1.7 2,228,544 + BT1782 0.70 +1.4 2,228,545 - BT1782 0.70 -0.9 2,228,575 - BT1782 0.73 -0.9 2,228,576 + BT1782 0.74 -1.2 2,228,577 - BT1782 0.74 +1.1 2,228,577 - BT1782 0.74 -0.9 2,228,583 - BT1782 0.74 -2.6 2,228,605 - BT1782 0.77 +0.7 2,228,639 + BT1782 0.81 +3.1 2,228,682 - BT1782 0.86 +0.4 2,228,695 - BT1782 0.88 -1.9 2,228,707 + BT1782 0.89 +1.3 2,228,712 - BT1782 0.90 +2.3 2,228,735 - -0.9 2,228,762 - -1.6 2,228,762 - +2.4 2,228,764 + -1.0 2,228,765 - -0.9 2,228,812 + +2.1 2,228,885 - -1.7 2,228,886 + +0.1 2,228,967 - -2.5 2,228,980 - +0.8 2,229,072 - BT1783 0.13 +1.1 2,229,073 + BT1783 0.13 -1.9 2,229,073 + BT1783 0.13 -0.5 2,229,090 - BT1783 0.14 +1.7 2,229,096 - BT1783 0.14 +2.4 2,229,148 + BT1783 0.17 -1.5 2,229,149 - BT1783 0.17 +2.1 2,229,227 + BT1783 0.20 -3.4 2,229,232 + BT1783 0.20 -0.9 2,229,233 - BT1783 0.20 -2.8 2,229,233 - BT1783 0.20 -1.9 2,229,333 - BT1783 0.25 +1.0 2,229,463 + BT1783 0.31 -2.5 2,229,463 + BT1783 0.31 -2.1 2,229,464 - BT1783 0.31 -1.2 2,229,464 - BT1783 0.31 -0.9 2,229,464 - BT1783 0.31 -1.7 2,229,465 + BT1783 0.31 +0.1 2,229,465 + BT1783 0.31 +0.6 2,229,466 - BT1783 0.31 -3.5 2,229,646 + BT1783 0.39 -1.3 2,229,668 - BT1783 0.40 +2.3 2,229,782 - BT1783 0.45 +1.3
Or see this region's nucleotide sequence