Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1491 and BT1492 are separated by 339 nucleotides BT1492 and BT1493 are separated by 109 nucleotides BT1493 and BT1494 are separated by 8 nucleotides BT1494 and BT1495 are separated by 72 nucleotides
BT1491: BT1491 - hypothetical protein (NCBI ptt file), at 1,840,776 to 1,841,546
BT1491
BT1492: BT1492 - tyrosine phenol-lyase (NCBI ptt file), at 1,841,886 to 1,843,265
BT1492
BT1493: BT1493 - conserved hypothetical protein (NCBI ptt file), at 1,843,375 to 1,843,677
BT1493
BT1494: BT1494 - conserved hypothetical protein (NCBI ptt file), at 1,843,686 to 1,843,910
BT1494
BT1495: BT1495 - siderophore (surfactin) biosynthesis regulatory protein (NCBI ptt file), at 1,843,983 to 1,844,588
BT1495
Position (kb)
1841
1842
1843
1844 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1840.982 kb on - strand, within BT1491 at 1840.988 kb on - strand, within BT1491 at 1841.097 kb on - strand, within BT1491 at 1841.113 kb on - strand, within BT1491 at 1841.126 kb on + strand, within BT1491 at 1841.163 kb on - strand, within BT1491 at 1841.168 kb on + strand, within BT1491 at 1841.205 kb on - strand, within BT1491 at 1841.211 kb on + strand, within BT1491 at 1841.222 kb on - strand, within BT1491 at 1841.237 kb on + strand, within BT1491 at 1841.257 kb on - strand, within BT1491 at 1841.257 kb on - strand, within BT1491 at 1841.309 kb on - strand, within BT1491 at 1841.399 kb on + strand, within BT1491 at 1841.402 kb on + strand, within BT1491 at 1841.403 kb on - strand, within BT1491 at 1841.430 kb on - strand, within BT1491 at 1841.447 kb on + strand, within BT1491 at 1841.448 kb on - strand, within BT1491 at 1841.469 kb on - strand, within BT1491 at 1841.469 kb on - strand, within BT1491 at 1841.499 kb on - strand at 1841.499 kb on - strand at 1841.581 kb on - strand at 1841.624 kb on + strand at 1841.624 kb on + strand at 1841.657 kb on - strand at 1841.673 kb on - strand at 1841.733 kb on + strand at 1841.751 kb on - strand at 1841.754 kb on + strand at 1841.755 kb on - strand at 1841.757 kb on - strand at 1841.757 kb on - strand at 1841.833 kb on + strand at 1841.985 kb on + strand at 1841.985 kb on + strand at 1841.986 kb on - strand at 1841.986 kb on - strand at 1841.986 kb on - strand at 1842.127 kb on + strand, within BT1492 at 1842.128 kb on - strand, within BT1492 at 1842.134 kb on + strand, within BT1492 at 1842.221 kb on - strand, within BT1492 at 1842.233 kb on + strand, within BT1492 at 1842.233 kb on + strand, within BT1492 at 1842.234 kb on - strand, within BT1492 at 1842.309 kb on - strand, within BT1492 at 1842.329 kb on + strand, within BT1492 at 1842.330 kb on - strand, within BT1492 at 1842.425 kb on + strand, within BT1492 at 1842.425 kb on + strand, within BT1492 at 1842.431 kb on + strand, within BT1492 at 1842.432 kb on - strand, within BT1492 at 1842.504 kb on + strand, within BT1492 at 1842.504 kb on + strand, within BT1492 at 1842.708 kb on + strand, within BT1492 at 1842.708 kb on + strand, within BT1492 at 1842.709 kb on - strand, within BT1492 at 1842.748 kb on - strand, within BT1492 at 1842.748 kb on - strand, within BT1492 at 1842.751 kb on + strand, within BT1492 at 1842.840 kb on - strand, within BT1492 at 1842.850 kb on + strand, within BT1492 at 1842.855 kb on + strand, within BT1492 at 1842.856 kb on - strand, within BT1492 at 1843.013 kb on - strand, within BT1492 at 1843.032 kb on + strand, within BT1492 at 1843.033 kb on - strand, within BT1492 at 1843.033 kb on - strand, within BT1492 at 1843.091 kb on - strand, within BT1492 at 1843.121 kb on + strand, within BT1492 at 1843.121 kb on + strand, within BT1492 at 1843.122 kb on - strand, within BT1492 at 1843.271 kb on - strand at 1843.298 kb on - strand at 1843.344 kb on + strand at 1843.368 kb on + strand at 1843.368 kb on + strand at 1843.369 kb on - strand at 1843.369 kb on - strand at 1843.390 kb on - strand at 1843.459 kb on + strand, within BT1493 at 1843.459 kb on + strand, within BT1493 at 1843.459 kb on + strand, within BT1493 at 1843.494 kb on - strand, within BT1493 at 1843.610 kb on + strand, within BT1493 at 1843.618 kb on + strand, within BT1493 at 1843.619 kb on - strand, within BT1493 at 1843.627 kb on - strand, within BT1493 at 1843.653 kb on - strand at 1843.672 kb on - strand at 1843.676 kb on - strand at 1843.682 kb on - strand at 1843.682 kb on - strand at 1843.721 kb on - strand, within BT1494 at 1843.768 kb on + strand, within BT1494 at 1843.788 kb on + strand, within BT1494 at 1843.830 kb on - strand, within BT1494 at 1843.843 kb on + strand, within BT1494 at 1843.843 kb on + strand, within BT1494 at 1843.905 kb on - strand at 1843.918 kb on + strand at 1843.953 kb on - strand at 1843.964 kb on - strand at 1843.969 kb on + strand at 1843.969 kb on + strand at 1843.992 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 1,840,982 - BT1491 0.27 +0.9 1,840,988 - BT1491 0.27 +2.7 1,841,097 - BT1491 0.42 -1.8 1,841,113 - BT1491 0.44 +2.9 1,841,126 + BT1491 0.45 -1.1 1,841,163 - BT1491 0.50 -2.1 1,841,168 + BT1491 0.51 +1.0 1,841,205 - BT1491 0.56 -1.7 1,841,211 + BT1491 0.56 -2.5 1,841,222 - BT1491 0.58 +0.5 1,841,237 + BT1491 0.60 -0.7 1,841,257 - BT1491 0.62 +0.8 1,841,257 - BT1491 0.62 -1.0 1,841,309 - BT1491 0.69 -0.8 1,841,399 + BT1491 0.81 +0.6 1,841,402 + BT1491 0.81 +0.2 1,841,403 - BT1491 0.81 -2.7 1,841,430 - BT1491 0.85 -0.7 1,841,447 + BT1491 0.87 -0.1 1,841,448 - BT1491 0.87 -1.7 1,841,469 - BT1491 0.90 -1.7 1,841,469 - BT1491 0.90 +2.4 1,841,499 - -0.1 1,841,499 - -2.1 1,841,581 - +1.2 1,841,624 + +0.2 1,841,624 + +0.6 1,841,657 - -0.1 1,841,673 - -2.6 1,841,733 + -0.5 1,841,751 - +1.7 1,841,754 + -1.1 1,841,755 - +1.8 1,841,757 - +1.7 1,841,757 - +1.4 1,841,833 + -0.1 1,841,985 + -2.5 1,841,985 + +0.6 1,841,986 - -2.4 1,841,986 - +1.1 1,841,986 - -0.8 1,842,127 + BT1492 0.17 -2.9 1,842,128 - BT1492 0.18 +0.5 1,842,134 + BT1492 0.18 +2.2 1,842,221 - BT1492 0.24 -0.1 1,842,233 + BT1492 0.25 -0.1 1,842,233 + BT1492 0.25 +0.5 1,842,234 - BT1492 0.25 -2.1 1,842,309 - BT1492 0.31 -2.4 1,842,329 + BT1492 0.32 -1.0 1,842,330 - BT1492 0.32 -1.7 1,842,425 + BT1492 0.39 -1.8 1,842,425 + BT1492 0.39 -1.6 1,842,431 + BT1492 0.39 -2.1 1,842,432 - BT1492 0.40 -0.4 1,842,504 + BT1492 0.45 -1.1 1,842,504 + BT1492 0.45 +3.8 1,842,708 + BT1492 0.60 -0.5 1,842,708 + BT1492 0.60 -1.0 1,842,709 - BT1492 0.60 -0.1 1,842,748 - BT1492 0.62 +3.1 1,842,748 - BT1492 0.62 +0.9 1,842,751 + BT1492 0.63 +0.9 1,842,840 - BT1492 0.69 -2.3 1,842,850 + BT1492 0.70 -1.2 1,842,855 + BT1492 0.70 -0.7 1,842,856 - BT1492 0.70 -2.1 1,843,013 - BT1492 0.82 -1.2 1,843,032 + BT1492 0.83 -2.9 1,843,033 - BT1492 0.83 -1.1 1,843,033 - BT1492 0.83 -2.1 1,843,091 - BT1492 0.87 +0.3 1,843,121 + BT1492 0.89 +0.6 1,843,121 + BT1492 0.89 -1.1 1,843,122 - BT1492 0.90 +2.5 1,843,271 - -2.1 1,843,298 - -0.1 1,843,344 + -2.8 1,843,368 + -2.7 1,843,368 + -1.9 1,843,369 - -0.1 1,843,369 - -2.7 1,843,390 - -1.1 1,843,459 + BT1493 0.28 +2.9 1,843,459 + BT1493 0.28 -2.0 1,843,459 + BT1493 0.28 +2.5 1,843,494 - BT1493 0.39 -1.1 1,843,610 + BT1493 0.78 -2.0 1,843,618 + BT1493 0.80 +2.2 1,843,619 - BT1493 0.81 -1.1 1,843,627 - BT1493 0.83 +1.0 1,843,653 - -1.7 1,843,672 - +1.5 1,843,676 - +1.5 1,843,682 - -0.9 1,843,682 - -1.0 1,843,721 - BT1494 0.16 -0.1 1,843,768 + BT1494 0.36 -2.1 1,843,788 + BT1494 0.45 -2.9 1,843,830 - BT1494 0.64 +1.9 1,843,843 + BT1494 0.70 -0.1 1,843,843 + BT1494 0.70 -0.1 1,843,905 - -1.1 1,843,918 + -1.3 1,843,953 - +0.8 1,843,964 - +1.4 1,843,969 + +1.9 1,843,969 + -2.1 1,843,992 - -1.3
Or see this region's nucleotide sequence