Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1320 and BT1321 are separated by 57 nucleotides BT1321 and BT1322 are separated by 7 nucleotides BT1322 and BT1323 are separated by 1 nucleotides
BT1320: BT1320 - putative transcriptional regulator, phosphate uptake regulation (NCBI ptt file), at 1,645,521 to 1,646,210
BT1320
BT1321: BT1321 - putative phosphate transport ATP-binding protein (NCBI ptt file), at 1,646,268 to 1,647,026
BT1321
BT1322: BT1322 - putative ABC transporter permease protein (NCBI ptt file), at 1,647,034 to 1,647,909
BT1322
BT1323: BT1323 - putative ABC transporter permease protein (NCBI ptt file), at 1,647,911 to 1,649,107
BT1323
Position (kb)
1647
1648 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1646.184 kb on - strand at 1646.224 kb on - strand at 1646.236 kb on + strand at 1646.291 kb on - strand at 1646.337 kb on - strand at 1646.425 kb on - strand, within BT1321 at 1646.435 kb on - strand, within BT1321 at 1646.478 kb on - strand, within BT1321 at 1646.532 kb on + strand, within BT1321 at 1646.709 kb on + strand, within BT1321 at 1646.724 kb on - strand, within BT1321 at 1646.807 kb on + strand, within BT1321 at 1646.808 kb on - strand, within BT1321 at 1646.810 kb on + strand, within BT1321 at 1646.984 kb on - strand at 1647.026 kb on + strand at 1647.131 kb on + strand, within BT1322 at 1647.132 kb on - strand, within BT1322 at 1647.132 kb on - strand, within BT1322 at 1647.142 kb on + strand, within BT1322 at 1647.142 kb on + strand, within BT1322 at 1647.183 kb on - strand, within BT1322 at 1647.204 kb on + strand, within BT1322 at 1647.245 kb on + strand, within BT1322 at 1647.307 kb on + strand, within BT1322 at 1647.790 kb on + strand, within BT1322 at 1647.792 kb on + strand, within BT1322 at 1647.829 kb on - strand at 1647.894 kb on + strand at 1647.920 kb on - strand at 1647.945 kb on + strand at 1647.945 kb on + strand at 1647.968 kb on + strand at 1647.968 kb on + strand at 1647.986 kb on + strand at 1648.210 kb on + strand, within BT1323 at 1648.216 kb on + strand, within BT1323 at 1648.218 kb on + strand, within BT1323 at 1648.218 kb on + strand, within BT1323 at 1648.218 kb on + strand, within BT1323 at 1648.220 kb on + strand, within BT1323 at 1648.315 kb on + strand, within BT1323 at 1648.342 kb on + strand, within BT1323 at 1648.444 kb on + strand, within BT1323 at 1648.517 kb on - strand, within BT1323 at 1648.618 kb on + strand, within BT1323 at 1648.667 kb on - strand, within BT1323 at 1648.669 kb on + strand, within BT1323 at 1648.669 kb on + strand, within BT1323 at 1648.708 kb on + strand, within BT1323 at 1648.779 kb on + strand, within BT1323 at 1648.810 kb on + strand, within BT1323 at 1648.812 kb on + strand, within BT1323
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 1,646,184 - -1.7 1,646,224 - -1.7 1,646,236 + -2.1 1,646,291 - -1.1 1,646,337 - -2.4 1,646,425 - BT1321 0.21 -1.4 1,646,435 - BT1321 0.22 +0.3 1,646,478 - BT1321 0.28 -2.1 1,646,532 + BT1321 0.35 +0.5 1,646,709 + BT1321 0.58 -1.7 1,646,724 - BT1321 0.60 -2.7 1,646,807 + BT1321 0.71 +0.5 1,646,808 - BT1321 0.71 -1.1 1,646,810 + BT1321 0.71 -0.1 1,646,984 - -0.1 1,647,026 + -1.3 1,647,131 + BT1322 0.11 +0.6 1,647,132 - BT1322 0.11 +1.3 1,647,132 - BT1322 0.11 +2.2 1,647,142 + BT1322 0.12 -1.0 1,647,142 + BT1322 0.12 +1.5 1,647,183 - BT1322 0.17 +1.9 1,647,204 + BT1322 0.19 -1.1 1,647,245 + BT1322 0.24 -1.7 1,647,307 + BT1322 0.31 -0.1 1,647,790 + BT1322 0.86 +0.5 1,647,792 + BT1322 0.87 +2.0 1,647,829 - -0.5 1,647,894 + -1.1 1,647,920 - -1.1 1,647,945 + -0.5 1,647,945 + -0.7 1,647,968 + +0.7 1,647,968 + -1.1 1,647,986 + -1.7 1,648,210 + BT1323 0.25 +1.5 1,648,216 + BT1323 0.25 -2.4 1,648,218 + BT1323 0.26 -0.1 1,648,218 + BT1323 0.26 -1.5 1,648,218 + BT1323 0.26 -3.4 1,648,220 + BT1323 0.26 -2.9 1,648,315 + BT1323 0.34 +2.5 1,648,342 + BT1323 0.36 +1.9 1,648,444 + BT1323 0.45 -0.1 1,648,517 - BT1323 0.51 +1.2 1,648,618 + BT1323 0.59 -1.9 1,648,667 - BT1323 0.63 -0.1 1,648,669 + BT1323 0.63 -2.7 1,648,669 + BT1323 0.63 +2.0 1,648,708 + BT1323 0.67 -0.1 1,648,779 + BT1323 0.73 -1.1 1,648,810 + BT1323 0.75 -0.1 1,648,812 + BT1323 0.75 +1.7
Or see this region's nucleotide sequence