Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1030 and BT1031 are separated by 527 nucleotides BT1031 and BT1032 are separated by 299 nucleotides
BT1030: BT1030 - hypothetical protein (NCBI ptt file), at 1,296,898 to 1,298,373
BT1030
BT1031: BT1031 - conserved hypothetical protein (NCBI ptt file), at 1,298,901 to 1,299,794
BT1031
BT1032: BT1032 - conserved hypothetical protein (NCBI ptt file), at 1,300,094 to 1,302,364
BT1032
Position (kb)
1298
1299
1300 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1297.953 kb on - strand, within BT1030 at 1298.025 kb on + strand, within BT1030 at 1298.055 kb on + strand, within BT1030 at 1298.067 kb on + strand, within BT1030 at 1298.092 kb on + strand, within BT1030 at 1298.155 kb on + strand, within BT1030 at 1298.311 kb on + strand at 1298.334 kb on + strand at 1298.365 kb on - strand at 1298.386 kb on - strand at 1298.520 kb on + strand at 1298.572 kb on + strand at 1298.638 kb on - strand at 1298.691 kb on + strand at 1298.778 kb on - strand at 1298.847 kb on + strand at 1298.848 kb on - strand at 1298.857 kb on + strand at 1298.858 kb on - strand at 1298.879 kb on - strand at 1298.883 kb on - strand at 1298.894 kb on + strand at 1298.898 kb on - strand at 1298.903 kb on - strand at 1298.992 kb on - strand, within BT1031 at 1299.042 kb on - strand, within BT1031 at 1299.042 kb on - strand, within BT1031 at 1299.140 kb on + strand, within BT1031 at 1299.140 kb on + strand, within BT1031 at 1299.141 kb on - strand, within BT1031 at 1299.141 kb on - strand, within BT1031 at 1299.145 kb on - strand, within BT1031 at 1299.184 kb on + strand, within BT1031 at 1299.265 kb on - strand, within BT1031 at 1299.503 kb on + strand, within BT1031 at 1299.768 kb on - strand at 1299.897 kb on + strand at 1299.909 kb on - strand at 1299.926 kb on + strand at 1299.973 kb on - strand at 1299.973 kb on - strand at 1299.973 kb on - strand at 1299.974 kb on + strand at 1300.078 kb on + strand at 1300.079 kb on - strand at 1300.095 kb on + strand at 1300.096 kb on - strand at 1300.110 kb on - strand at 1300.121 kb on - strand at 1300.340 kb on + strand, within BT1032 at 1300.345 kb on + strand, within BT1032 at 1300.373 kb on - strand, within BT1032 at 1300.373 kb on - strand, within BT1032 at 1300.373 kb on - strand, within BT1032 at 1300.420 kb on - strand, within BT1032 at 1300.471 kb on + strand, within BT1032 at 1300.601 kb on + strand, within BT1032 at 1300.601 kb on + strand, within BT1032 at 1300.601 kb on + strand, within BT1032 at 1300.614 kb on - strand, within BT1032 at 1300.617 kb on + strand, within BT1032 at 1300.618 kb on - strand, within BT1032 at 1300.756 kb on - strand, within BT1032 at 1300.762 kb on + strand, within BT1032 at 1300.764 kb on + strand, within BT1032 at 1300.780 kb on + strand, within BT1032
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 1,297,953 - BT1030 0.71 -2.0 1,298,025 + BT1030 0.76 +0.3 1,298,055 + BT1030 0.78 -1.0 1,298,067 + BT1030 0.79 -1.5 1,298,092 + BT1030 0.81 -1.5 1,298,155 + BT1030 0.85 +1.1 1,298,311 + -0.1 1,298,334 + -1.1 1,298,365 - +1.5 1,298,386 - -2.1 1,298,520 + +2.6 1,298,572 + -0.7 1,298,638 - -0.2 1,298,691 + -0.8 1,298,778 - -2.4 1,298,847 + -1.7 1,298,848 - -0.4 1,298,857 + +1.3 1,298,858 - +1.6 1,298,879 - +3.1 1,298,883 - -1.1 1,298,894 + -0.4 1,298,898 - -1.0 1,298,903 - +3.3 1,298,992 - BT1031 0.10 -0.4 1,299,042 - BT1031 0.16 -3.2 1,299,042 - BT1031 0.16 +0.9 1,299,140 + BT1031 0.27 -2.0 1,299,140 + BT1031 0.27 +1.6 1,299,141 - BT1031 0.27 -3.2 1,299,141 - BT1031 0.27 +0.6 1,299,145 - BT1031 0.27 -1.4 1,299,184 + BT1031 0.32 -2.3 1,299,265 - BT1031 0.41 -0.8 1,299,503 + BT1031 0.67 -1.4 1,299,768 - -1.1 1,299,897 + +1.9 1,299,909 - -1.4 1,299,926 + -0.4 1,299,973 - -1.7 1,299,973 - -0.4 1,299,973 - -2.2 1,299,974 + -0.0 1,300,078 + -1.8 1,300,079 - -1.8 1,300,095 + -0.4 1,300,096 - -2.0 1,300,110 - +1.6 1,300,121 - -0.4 1,300,340 + BT1032 0.11 -1.8 1,300,345 + BT1032 0.11 -1.2 1,300,373 - BT1032 0.12 -1.4 1,300,373 - BT1032 0.12 +0.1 1,300,373 - BT1032 0.12 -1.4 1,300,420 - BT1032 0.14 -3.0 1,300,471 + BT1032 0.17 -2.8 1,300,601 + BT1032 0.22 +1.0 1,300,601 + BT1032 0.22 -2.8 1,300,601 + BT1032 0.22 +0.6 1,300,614 - BT1032 0.23 -0.4 1,300,617 + BT1032 0.23 +0.1 1,300,618 - BT1032 0.23 -1.4 1,300,756 - BT1032 0.29 -3.0 1,300,762 + BT1032 0.29 -1.4 1,300,764 + BT1032 0.30 -1.0 1,300,780 + BT1032 0.30 -0.0
Or see this region's nucleotide sequence