Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0736 and BT0737 are separated by 206 nucleotides BT0737 and BT0738 are separated by 386 nucleotides
BT0736: BT0736 - putative transport protein (NCBI ptt file), at 912,166 to 913,860
BT0736
BT0737: BT0737 - formate--tetrahydrofolate ligase (NCBI ptt file), at 914,067 to 915,734
BT0737
BT0738: BT0738 - serine hydroxymethyltransferase (NCBI ptt file), at 916,121 to 917,401
BT0738
Position (kb) 914
915
916 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 913.376 kb on + strand, within BT0736 at 913.510 kb on + strand, within BT0736 at 913.510 kb on + strand, within BT0736 at 913.537 kb on + strand, within BT0736 at 913.586 kb on - strand, within BT0736 at 913.634 kb on - strand, within BT0736 at 913.714 kb on + strand at 913.802 kb on - strand at 913.848 kb on + strand at 913.862 kb on + strand at 913.862 kb on + strand at 913.877 kb on + strand at 913.956 kb on + strand at 913.963 kb on + strand at 914.081 kb on - strand at 914.284 kb on + strand, within BT0737 at 914.284 kb on + strand, within BT0737 at 914.427 kb on + strand, within BT0737 at 914.742 kb on - strand, within BT0737 at 914.745 kb on + strand, within BT0737 at 914.944 kb on - strand, within BT0737 at 914.996 kb on + strand, within BT0737 at 915.146 kb on + strand, within BT0737 at 915.159 kb on - strand, within BT0737 at 915.270 kb on + strand, within BT0737 at 915.338 kb on + strand, within BT0737 at 915.381 kb on - strand, within BT0737 at 915.537 kb on + strand, within BT0737 at 915.538 kb on - strand, within BT0737 at 915.602 kb on + strand at 915.605 kb on - strand at 915.659 kb on + strand at 915.660 kb on - strand at 915.784 kb on - strand at 915.834 kb on - strand at 915.838 kb on - strand at 915.849 kb on + strand at 915.880 kb on + strand at 915.911 kb on - strand at 915.913 kb on - strand at 915.966 kb on + strand at 916.032 kb on + strand at 916.080 kb on - strand at 916.173 kb on - strand at 916.282 kb on + strand, within BT0738 at 916.387 kb on + strand, within BT0738 at 916.388 kb on - strand, within BT0738 at 916.388 kb on - strand, within BT0738 at 916.580 kb on + strand, within BT0738 at 916.581 kb on - strand, within BT0738 at 916.605 kb on - strand, within BT0738
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 913,376 + BT0736 0.71 +1.3 913,510 + BT0736 0.79 +1.6 913,510 + BT0736 0.79 +1.2 913,537 + BT0736 0.81 -1.1 913,586 - BT0736 0.84 +0.2 913,634 - BT0736 0.87 -3.7 913,714 + +0.6 913,802 - -0.1 913,848 + +2.6 913,862 + +1.6 913,862 + +0.4 913,877 + +1.9 913,956 + -0.2 913,963 + -0.1 914,081 - -2.4 914,284 + BT0737 0.13 -1.4 914,284 + BT0737 0.13 +0.6 914,427 + BT0737 0.22 -1.4 914,742 - BT0737 0.40 -1.4 914,745 + BT0737 0.41 -1.4 914,944 - BT0737 0.53 -1.8 914,996 + BT0737 0.56 -3.2 915,146 + BT0737 0.65 -2.9 915,159 - BT0737 0.65 -3.6 915,270 + BT0737 0.72 -2.4 915,338 + BT0737 0.76 -4.0 915,381 - BT0737 0.79 +1.2 915,537 + BT0737 0.88 -1.6 915,538 - BT0737 0.88 -1.4 915,602 + -2.0 915,605 - -4.7 915,659 + -3.1 915,660 - -0.4 915,784 - -1.4 915,834 - -2.4 915,838 - -0.4 915,849 + -2.4 915,880 + -3.2 915,911 - -3.2 915,913 - +1.6 915,966 + -0.4 916,032 + +1.6 916,080 - -0.4 916,173 - +0.3 916,282 + BT0738 0.13 +0.2 916,387 + BT0738 0.21 +3.2 916,388 - BT0738 0.21 -1.4 916,388 - BT0738 0.21 -0.8 916,580 + BT0738 0.36 -0.4 916,581 - BT0738 0.36 -1.1 916,605 - BT0738 0.38 -0.2
Or see this region's nucleotide sequence