Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0721 and BT0722 are separated by 98 nucleotides BT0722 and BT0723 overlap by 4 nucleotides BT0723 and BT0724 are separated by 13 nucleotides BT0724 and BT0725 are separated by 303 nucleotides
BT0721: BT0721 - DNA repair and recombination protein, putative helicase (NCBI ptt file), at 894,960 to 897,506
BT0721
BT0722: BT0722 - conserved hypothetical protein, putative surface protein, function unknown (NCBI ptt file), at 897,605 to 898,225
BT0722
BT0723: BT0723 - hypothetical protein (NCBI ptt file), at 898,222 to 898,500
BT0723
BT0724: BT0724 - hypothetical protein (NCBI ptt file), at 898,514 to 898,726
BT0724
BT0725: BT0725 - dTDP-4-dehydrorhamnose reductase (NCBI ptt file), at 899,030 to 899,938
BT0725
Position (kb) 898
899 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 897.365 kb on - strand at 897.383 kb on - strand at 897.588 kb on + strand at 897.606 kb on + strand at 898.121 kb on + strand, within BT0722 at 898.125 kb on - strand at 898.128 kb on + strand, within BT0722 at 898.142 kb on - strand, within BT0722 at 898.387 kb on + strand, within BT0723 at 898.430 kb on - strand, within BT0723 at 898.482 kb on + strand at 898.483 kb on - strand at 898.506 kb on - strand at 898.570 kb on - strand, within BT0724 at 898.752 kb on + strand at 898.890 kb on - strand at 898.909 kb on + strand at 898.914 kb on + strand at 898.987 kb on + strand at 898.988 kb on - strand at 899.014 kb on + strand at 899.103 kb on - strand at 899.310 kb on - strand, within BT0725 at 899.452 kb on - strand, within BT0725
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 897,365 - +0.1 897,383 - -1.0 897,588 + -1.4 897,606 + +1.2 898,121 + BT0722 0.83 -1.4 898,125 - -0.4 898,128 + BT0722 0.84 -2.9 898,142 - BT0722 0.86 -2.4 898,387 + BT0723 0.59 -3.7 898,430 - BT0723 0.75 -2.0 898,482 + -2.0 898,483 - -2.0 898,506 - -1.2 898,570 - BT0724 0.26 +0.3 898,752 + -0.4 898,890 - +3.2 898,909 + -0.6 898,914 + -1.2 898,987 + -0.7 898,988 - -0.4 899,014 + -0.1 899,103 - -1.7 899,310 - BT0725 0.31 -1.2 899,452 - BT0725 0.46 +2.2
Or see this region's nucleotide sequence