Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0661 and BT0662 are separated by 359 nucleotides BT0662 and BT0663 are separated by 16 nucleotides BT0663 and BT0664 are separated by 29 nucleotides
BT0661: BT0661 - hypothetical protein (NCBI ptt file), at 814,865 to 816,091
BT0661
BT0662: BT0662 - hypothetical protein (NCBI ptt file), at 816,451 to 818,247
BT0662
BT0663: BT0663 - hypothetical protein (NCBI ptt file), at 818,264 to 818,710
BT0663
BT0664: BT0664 - hypothetical protein (NCBI ptt file), at 818,740 to 819,888
BT0664
Position (kb) 816
817
818
819 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 815.556 kb on + strand, within BT0661 at 815.570 kb on + strand, within BT0661 at 815.581 kb on - strand, within BT0661 at 815.637 kb on - strand, within BT0661 at 815.690 kb on - strand, within BT0661 at 815.916 kb on + strand, within BT0661 at 815.917 kb on - strand, within BT0661 at 815.924 kb on + strand, within BT0661 at 815.978 kb on - strand at 816.033 kb on - strand at 816.064 kb on + strand at 816.363 kb on - strand at 816.365 kb on - strand at 816.366 kb on + strand at 816.366 kb on + strand at 816.367 kb on - strand at 816.417 kb on - strand at 816.417 kb on - strand at 816.541 kb on - strand at 816.541 kb on - strand at 816.544 kb on - strand at 816.588 kb on - strand at 816.625 kb on - strand at 816.688 kb on + strand, within BT0662 at 816.700 kb on + strand, within BT0662 at 816.701 kb on - strand, within BT0662 at 816.707 kb on - strand, within BT0662 at 816.745 kb on - strand, within BT0662 at 816.745 kb on - strand, within BT0662 at 816.766 kb on + strand, within BT0662 at 816.800 kb on + strand, within BT0662 at 816.843 kb on + strand, within BT0662 at 816.843 kb on + strand, within BT0662 at 816.853 kb on - strand, within BT0662 at 816.891 kb on + strand, within BT0662 at 816.954 kb on - strand, within BT0662 at 816.954 kb on - strand, within BT0662 at 816.997 kb on - strand, within BT0662 at 817.095 kb on + strand at 817.188 kb on + strand, within BT0662 at 817.201 kb on - strand, within BT0662 at 817.206 kb on + strand, within BT0662 at 817.292 kb on + strand, within BT0662 at 817.314 kb on - strand, within BT0662 at 817.345 kb on + strand, within BT0662 at 817.524 kb on + strand, within BT0662 at 817.535 kb on - strand, within BT0662 at 817.732 kb on - strand, within BT0662 at 817.783 kb on + strand, within BT0662 at 817.788 kb on + strand, within BT0662 at 817.789 kb on - strand, within BT0662 at 817.847 kb on + strand, within BT0662 at 817.873 kb on - strand, within BT0662 at 817.932 kb on - strand, within BT0662 at 817.941 kb on - strand, within BT0662 at 817.978 kb on + strand, within BT0662 at 818.151 kb on + strand at 818.152 kb on - strand at 818.230 kb on - strand at 818.238 kb on + strand at 818.301 kb on + strand at 818.313 kb on + strand, within BT0663 at 818.399 kb on - strand, within BT0663 at 818.461 kb on + strand, within BT0663 at 818.462 kb on - strand, within BT0663 at 818.462 kb on - strand, within BT0663 at 818.489 kb on - strand, within BT0663 at 818.605 kb on + strand, within BT0663 at 818.606 kb on + strand, within BT0663 at 818.709 kb on - strand at 818.719 kb on - strand at 818.723 kb on - strand at 818.790 kb on - strand at 818.842 kb on - strand at 818.937 kb on - strand, within BT0664 at 819.061 kb on - strand, within BT0664
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 815,556 + BT0661 0.56 +4.0 815,570 + BT0661 0.57 -1.2 815,581 - BT0661 0.58 +0.4 815,637 - BT0661 0.63 -2.9 815,690 - BT0661 0.67 -0.3 815,916 + BT0661 0.86 +1.3 815,917 - BT0661 0.86 -1.9 815,924 + BT0661 0.86 -0.1 815,978 - +1.0 816,033 - +0.3 816,064 + +2.3 816,363 - -2.6 816,365 - -0.8 816,366 + -2.2 816,366 + -1.3 816,367 - -0.6 816,417 - -1.3 816,417 - -2.3 816,541 - -0.3 816,541 - +2.9 816,544 - -0.9 816,588 - -0.3 816,625 - +2.7 816,688 + BT0662 0.13 -1.3 816,700 + BT0662 0.14 -0.3 816,701 - BT0662 0.14 +0.7 816,707 - BT0662 0.14 +1.3 816,745 - BT0662 0.16 -1.9 816,745 - BT0662 0.16 +0.7 816,766 + BT0662 0.18 -0.9 816,800 + BT0662 0.19 -0.3 816,843 + BT0662 0.22 +0.3 816,843 + BT0662 0.22 +0.7 816,853 - BT0662 0.22 -0.3 816,891 + BT0662 0.24 +0.7 816,954 - BT0662 0.28 -0.3 816,954 - BT0662 0.28 -2.9 816,997 - BT0662 0.30 +2.3 817,095 + -1.9 817,188 + BT0662 0.41 +1.7 817,201 - BT0662 0.42 +0.0 817,206 + BT0662 0.42 +0.7 817,292 + BT0662 0.47 -1.3 817,314 - BT0662 0.48 +1.3 817,345 + BT0662 0.50 -2.1 817,524 + BT0662 0.60 +0.7 817,535 - BT0662 0.60 -2.4 817,732 - BT0662 0.71 +0.4 817,783 + BT0662 0.74 +0.2 817,788 + BT0662 0.74 +0.5 817,789 - BT0662 0.74 -2.1 817,847 + BT0662 0.78 -0.3 817,873 - BT0662 0.79 +0.1 817,932 - BT0662 0.82 +0.2 817,941 - BT0662 0.83 +0.4 817,978 + BT0662 0.85 +0.3 818,151 + +0.1 818,152 - +0.7 818,230 - -1.7 818,238 + -1.3 818,301 + -0.7 818,313 + BT0663 0.11 +3.0 818,399 - BT0663 0.30 -0.6 818,461 + BT0663 0.44 -1.6 818,462 - BT0663 0.44 +1.9 818,462 - BT0663 0.44 +0.6 818,489 - BT0663 0.50 -1.1 818,605 + BT0663 0.76 -1.3 818,606 + BT0663 0.77 +2.7 818,709 - +1.3 818,719 - +1.7 818,723 - -1.3 818,790 - +1.0 818,842 - -1.3 818,937 - BT0664 0.17 -0.7 819,061 - BT0664 0.28 +1.3
Or see this region's nucleotide sequence