Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0017 and BT0018 are separated by 182 nucleotides BT0018 and BT0019 are separated by 11 nucleotides BT0019 and BT0020 are separated by 6 nucleotides BT0020 and BT0021 are separated by 82 nucleotides
BT0017: BT0017 - hypothetical protein (NCBI ptt file), at 15,525 to 16,472
BT0017
BT0018: BT0018 - hypothetical protein (NCBI ptt file), at 16,655 to 17,371
BT0018
BT0019: BT0019 - hypothetical protein (NCBI ptt file), at 17,383 to 17,703
BT0019
BT0020: BT0020 - hypothetical protein (NCBI ptt file), at 17,710 to 18,168
BT0020
BT0021: BT0021 - hypothetical protein (NCBI ptt file), at 18,251 to 18,919
BT0021
Position (kb)
17
18 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 16.462 kb on + strand at 16.521 kb on - strand at 16.521 kb on - strand at 16.521 kb on - strand at 16.699 kb on - strand at 16.914 kb on + strand, within BT0018 at 16.971 kb on + strand, within BT0018 at 17.241 kb on + strand, within BT0018 at 17.287 kb on - strand, within BT0018 at 17.287 kb on - strand, within BT0018 at 17.398 kb on - strand at 17.554 kb on + strand, within BT0019 at 17.554 kb on + strand, within BT0019 at 17.555 kb on - strand, within BT0019 at 17.558 kb on - strand, within BT0019 at 17.583 kb on - strand, within BT0019 at 17.668 kb on + strand, within BT0019 at 17.692 kb on + strand at 17.693 kb on - strand at 17.738 kb on - strand at 17.751 kb on + strand at 17.775 kb on - strand, within BT0020 at 17.837 kb on - strand, within BT0020 at 17.839 kb on + strand, within BT0020 at 17.840 kb on - strand, within BT0020 at 17.840 kb on - strand, within BT0020 at 17.914 kb on + strand, within BT0020 at 17.926 kb on + strand, within BT0020 at 17.926 kb on + strand, within BT0020 at 17.927 kb on - strand, within BT0020 at 18.096 kb on - strand, within BT0020 at 18.125 kb on - strand at 18.188 kb on - strand at 18.209 kb on + strand at 18.210 kb on - strand at 18.233 kb on - strand at 18.233 kb on - strand at 18.235 kb on - strand at 18.235 kb on - strand at 18.237 kb on - strand at 18.358 kb on - strand, within BT0021 at 18.486 kb on - strand, within BT0021 at 18.607 kb on + strand, within BT0021 at 18.611 kb on - strand, within BT0021 at 18.638 kb on + strand, within BT0021 at 18.641 kb on + strand, within BT0021
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 16,462 + -1.7 16,521 - -0.9 16,521 - -1.2 16,521 - +0.2 16,699 - +2.1 16,914 + BT0018 0.36 +0.8 16,971 + BT0018 0.44 -1.6 17,241 + BT0018 0.82 -1.7 17,287 - BT0018 0.88 -1.4 17,287 - BT0018 0.88 -2.4 17,398 - -0.2 17,554 + BT0019 0.53 -2.2 17,554 + BT0019 0.53 -1.2 17,555 - BT0019 0.54 -4.9 17,558 - BT0019 0.55 -0.2 17,583 - BT0019 0.62 -0.9 17,668 + BT0019 0.89 -2.6 17,692 + -0.2 17,693 - -1.5 17,738 - +0.1 17,751 + -1.2 17,775 - BT0020 0.14 -3.2 17,837 - BT0020 0.28 +2.8 17,839 + BT0020 0.28 -0.4 17,840 - BT0020 0.28 -0.6 17,840 - BT0020 0.28 -1.5 17,914 + BT0020 0.44 +0.8 17,926 + BT0020 0.47 -3.2 17,926 + BT0020 0.47 +0.7 17,927 - BT0020 0.47 +0.8 18,096 - BT0020 0.84 -0.5 18,125 - -0.2 18,188 - -1.6 18,209 + +0.6 18,210 - +2.0 18,233 - -0.5 18,233 - +0.8 18,235 - -1.6 18,235 - +2.3 18,237 - +1.4 18,358 - BT0021 0.16 +1.4 18,486 - BT0021 0.35 -0.2 18,607 + BT0021 0.53 -1.4 18,611 - BT0021 0.54 -1.7 18,638 + BT0021 0.58 -3.0 18,641 + BT0021 0.58 -2.6
Or see this region's nucleotide sequence