Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4817 and Pf6N2E2_4818 are separated by 28 nucleotides Pf6N2E2_4818 and Pf6N2E2_4819 are separated by 139 nucleotides Pf6N2E2_4819 and Pf6N2E2_4820 are separated by 78 nucleotides Pf6N2E2_4820 and Pf6N2E2_4821 overlap by 4 nucleotides
Pf6N2E2_4817 - Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1), at 5,570,273 to 5,571,520
_4817
Pf6N2E2_4818 - Spermidine Putrescine ABC transporter permease component potC (TC_3.A.1.11.1), at 5,571,549 to 5,572,358
_4818
Pf6N2E2_4819 - Alcohol dehydrogenase (EC 1.1.1.1), at 5,572,498 to 5,573,646
_4819
Pf6N2E2_4820 - Ribulose-phosphate 3-epimerase (EC 5.1.3.1), at 5,573,725 to 5,574,399
_4820
Pf6N2E2_4821 - Phosphoglycolate phosphatase (EC 3.1.3.18), at 5,574,396 to 5,575,214
_4821
Position (kb) 5572
5573
5574 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5571.546 kb on + strand at 5571.546 kb on + strand at 5571.546 kb on + strand at 5571.546 kb on + strand at 5571.546 kb on + strand at 5571.546 kb on + strand at 5571.546 kb on + strand at 5571.546 kb on + strand at 5571.546 kb on + strand at 5571.546 kb on + strand at 5571.547 kb on - strand at 5571.547 kb on - strand at 5571.547 kb on - strand at 5571.547 kb on - strand at 5571.547 kb on - strand at 5571.547 kb on - strand at 5571.547 kb on - strand at 5571.576 kb on + strand at 5571.576 kb on + strand at 5571.576 kb on + strand at 5571.576 kb on + strand at 5571.577 kb on - strand at 5571.577 kb on - strand at 5571.577 kb on - strand at 5571.675 kb on + strand, within Pf6N2E2_4818 at 5571.705 kb on + strand, within Pf6N2E2_4818 at 5571.705 kb on + strand, within Pf6N2E2_4818 at 5571.706 kb on - strand, within Pf6N2E2_4818 at 5571.912 kb on + strand, within Pf6N2E2_4818 at 5571.913 kb on - strand, within Pf6N2E2_4818 at 5571.913 kb on - strand, within Pf6N2E2_4818 at 5571.913 kb on - strand, within Pf6N2E2_4818 at 5571.913 kb on - strand, within Pf6N2E2_4818 at 5571.985 kb on + strand, within Pf6N2E2_4818 at 5571.985 kb on + strand, within Pf6N2E2_4818 at 5571.986 kb on - strand, within Pf6N2E2_4818 at 5571.986 kb on - strand, within Pf6N2E2_4818 at 5572.343 kb on + strand at 5572.343 kb on + strand at 5572.344 kb on - strand at 5572.347 kb on - strand at 5572.363 kb on + strand at 5572.363 kb on + strand at 5572.390 kb on - strand at 5572.457 kb on + strand at 5572.457 kb on + strand at 5572.483 kb on + strand at 5572.484 kb on - strand at 5572.484 kb on - strand at 5572.528 kb on - strand at 5572.675 kb on + strand, within Pf6N2E2_4819 at 5572.675 kb on + strand, within Pf6N2E2_4819 at 5572.676 kb on - strand, within Pf6N2E2_4819 at 5572.676 kb on - strand, within Pf6N2E2_4819 at 5572.819 kb on + strand, within Pf6N2E2_4819 at 5572.819 kb on + strand, within Pf6N2E2_4819 at 5572.820 kb on - strand, within Pf6N2E2_4819 at 5572.820 kb on - strand, within Pf6N2E2_4819 at 5572.820 kb on - strand, within Pf6N2E2_4819 at 5572.820 kb on - strand, within Pf6N2E2_4819 at 5572.820 kb on - strand, within Pf6N2E2_4819 at 5572.820 kb on - strand, within Pf6N2E2_4819 at 5572.848 kb on + strand, within Pf6N2E2_4819 at 5572.848 kb on + strand, within Pf6N2E2_4819 at 5572.848 kb on + strand, within Pf6N2E2_4819 at 5572.849 kb on - strand, within Pf6N2E2_4819 at 5572.990 kb on + strand, within Pf6N2E2_4819 at 5572.990 kb on + strand, within Pf6N2E2_4819 at 5572.990 kb on + strand, within Pf6N2E2_4819 at 5572.990 kb on + strand, within Pf6N2E2_4819 at 5572.990 kb on + strand, within Pf6N2E2_4819 at 5572.990 kb on + strand, within Pf6N2E2_4819 at 5572.991 kb on - strand, within Pf6N2E2_4819 at 5572.991 kb on - strand, within Pf6N2E2_4819 at 5572.991 kb on - strand, within Pf6N2E2_4819 at 5572.991 kb on - strand, within Pf6N2E2_4819 at 5573.098 kb on + strand, within Pf6N2E2_4819 at 5573.098 kb on + strand, within Pf6N2E2_4819 at 5573.099 kb on - strand, within Pf6N2E2_4819 at 5573.182 kb on + strand, within Pf6N2E2_4819 at 5573.182 kb on + strand, within Pf6N2E2_4819 at 5573.182 kb on + strand, within Pf6N2E2_4819 at 5573.183 kb on - strand, within Pf6N2E2_4819 at 5573.183 kb on - strand, within Pf6N2E2_4819 at 5573.183 kb on - strand, within Pf6N2E2_4819 at 5573.337 kb on + strand, within Pf6N2E2_4819 at 5573.337 kb on + strand, within Pf6N2E2_4819 at 5573.337 kb on + strand, within Pf6N2E2_4819 at 5573.337 kb on + strand, within Pf6N2E2_4819 at 5573.337 kb on + strand, within Pf6N2E2_4819 at 5573.337 kb on + strand, within Pf6N2E2_4819 at 5573.337 kb on + strand, within Pf6N2E2_4819 at 5573.337 kb on + strand, within Pf6N2E2_4819 at 5573.337 kb on + strand, within Pf6N2E2_4819 at 5573.338 kb on - strand, within Pf6N2E2_4819 at 5573.338 kb on - strand, within Pf6N2E2_4819 at 5573.338 kb on - strand, within Pf6N2E2_4819 at 5573.338 kb on - strand, within Pf6N2E2_4819 at 5573.338 kb on - strand, within Pf6N2E2_4819 at 5573.338 kb on - strand, within Pf6N2E2_4819 at 5573.338 kb on - strand, within Pf6N2E2_4819 at 5573.338 kb on - strand, within Pf6N2E2_4819 at 5573.338 kb on - strand, within Pf6N2E2_4819 at 5573.350 kb on + strand, within Pf6N2E2_4819 at 5573.350 kb on + strand, within Pf6N2E2_4819 at 5573.351 kb on - strand, within Pf6N2E2_4819 at 5573.351 kb on - strand, within Pf6N2E2_4819 at 5573.475 kb on + strand, within Pf6N2E2_4819 at 5573.475 kb on + strand, within Pf6N2E2_4819 at 5573.476 kb on - strand, within Pf6N2E2_4819 at 5573.476 kb on - strand, within Pf6N2E2_4819 at 5573.476 kb on - strand, within Pf6N2E2_4819 at 5573.476 kb on - strand, within Pf6N2E2_4819 at 5573.476 kb on - strand, within Pf6N2E2_4819 at 5573.499 kb on - strand, within Pf6N2E2_4819 at 5573.570 kb on + strand at 5573.630 kb on - strand at 5573.641 kb on + strand at 5573.642 kb on - strand at 5573.681 kb on + strand at 5573.681 kb on + strand at 5573.683 kb on + strand at 5573.683 kb on + strand at 5573.683 kb on + strand at 5573.683 kb on + strand at 5573.683 kb on + strand at 5573.683 kb on + strand at 5573.683 kb on + strand at 5573.683 kb on + strand at 5573.683 kb on + strand at 5573.684 kb on - strand at 5573.684 kb on - strand at 5573.684 kb on - strand at 5573.684 kb on - strand at 5573.684 kb on - strand at 5574.550 kb on + strand, within Pf6N2E2_4821
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 5,571,546 + -0.7 5,571,546 + -1.1 5,571,546 + -2.7 5,571,546 + -1.8 5,571,546 + -1.6 5,571,546 + +0.0 5,571,546 + -0.4 5,571,546 + -1.3 5,571,546 + -0.3 5,571,546 + -1.5 5,571,547 - -1.3 5,571,547 - -0.7 5,571,547 - +0.2 5,571,547 - -2.2 5,571,547 - -2.5 5,571,547 - -1.2 5,571,547 - -1.4 5,571,576 + -1.3 5,571,576 + -0.8 5,571,576 + -1.2 5,571,576 + -2.2 5,571,577 - -1.2 5,571,577 - -1.5 5,571,577 - -2.3 5,571,675 + Pf6N2E2_4818 0.16 -1.0 5,571,705 + Pf6N2E2_4818 0.19 -3.4 5,571,705 + Pf6N2E2_4818 0.19 -0.4 5,571,706 - Pf6N2E2_4818 0.19 -2.5 5,571,912 + Pf6N2E2_4818 0.45 -1.4 5,571,913 - Pf6N2E2_4818 0.45 -0.6 5,571,913 - Pf6N2E2_4818 0.45 -0.9 5,571,913 - Pf6N2E2_4818 0.45 -0.6 5,571,913 - Pf6N2E2_4818 0.45 -1.0 5,571,985 + Pf6N2E2_4818 0.54 -1.3 5,571,985 + Pf6N2E2_4818 0.54 +0.0 5,571,986 - Pf6N2E2_4818 0.54 -0.9 5,571,986 - Pf6N2E2_4818 0.54 -1.2 5,572,343 + +0.6 5,572,343 + +0.4 5,572,344 - +1.0 5,572,347 - +0.1 5,572,363 + -0.5 5,572,363 + -0.4 5,572,390 - +0.0 5,572,457 + -0.5 5,572,457 + +1.1 5,572,483 + +0.5 5,572,484 - +0.5 5,572,484 - +0.6 5,572,528 - +0.3 5,572,675 + Pf6N2E2_4819 0.15 -0.3 5,572,675 + Pf6N2E2_4819 0.15 -0.1 5,572,676 - Pf6N2E2_4819 0.15 -0.6 5,572,676 - Pf6N2E2_4819 0.15 -0.3 5,572,819 + Pf6N2E2_4819 0.28 +0.5 5,572,819 + Pf6N2E2_4819 0.28 -0.2 5,572,820 - Pf6N2E2_4819 0.28 -0.7 5,572,820 - Pf6N2E2_4819 0.28 +0.3 5,572,820 - Pf6N2E2_4819 0.28 +0.7 5,572,820 - Pf6N2E2_4819 0.28 +0.4 5,572,820 - Pf6N2E2_4819 0.28 +0.0 5,572,820 - Pf6N2E2_4819 0.28 +0.1 5,572,848 + Pf6N2E2_4819 0.30 -0.3 5,572,848 + Pf6N2E2_4819 0.30 +0.6 5,572,848 + Pf6N2E2_4819 0.30 -0.0 5,572,849 - Pf6N2E2_4819 0.31 +0.0 5,572,990 + Pf6N2E2_4819 0.43 +0.4 5,572,990 + Pf6N2E2_4819 0.43 +0.5 5,572,990 + Pf6N2E2_4819 0.43 -0.0 5,572,990 + Pf6N2E2_4819 0.43 +0.4 5,572,990 + Pf6N2E2_4819 0.43 -1.7 5,572,990 + Pf6N2E2_4819 0.43 +0.6 5,572,991 - Pf6N2E2_4819 0.43 +1.6 5,572,991 - Pf6N2E2_4819 0.43 -0.5 5,572,991 - Pf6N2E2_4819 0.43 -0.2 5,572,991 - Pf6N2E2_4819 0.43 +0.8 5,573,098 + Pf6N2E2_4819 0.52 +0.6 5,573,098 + Pf6N2E2_4819 0.52 +0.4 5,573,099 - Pf6N2E2_4819 0.52 -0.2 5,573,182 + Pf6N2E2_4819 0.60 -0.4 5,573,182 + Pf6N2E2_4819 0.60 -2.2 5,573,182 + Pf6N2E2_4819 0.60 -1.1 5,573,183 - Pf6N2E2_4819 0.60 -0.2 5,573,183 - Pf6N2E2_4819 0.60 -0.1 5,573,183 - Pf6N2E2_4819 0.60 -0.9 5,573,337 + Pf6N2E2_4819 0.73 -0.3 5,573,337 + Pf6N2E2_4819 0.73 +0.7 5,573,337 + Pf6N2E2_4819 0.73 +0.7 5,573,337 + Pf6N2E2_4819 0.73 -1.5 5,573,337 + Pf6N2E2_4819 0.73 -0.0 5,573,337 + Pf6N2E2_4819 0.73 +0.8 5,573,337 + Pf6N2E2_4819 0.73 -1.3 5,573,337 + Pf6N2E2_4819 0.73 +0.1 5,573,337 + Pf6N2E2_4819 0.73 +0.6 5,573,338 - Pf6N2E2_4819 0.73 -1.4 5,573,338 - Pf6N2E2_4819 0.73 -1.5 5,573,338 - Pf6N2E2_4819 0.73 -0.4 5,573,338 - Pf6N2E2_4819 0.73 -0.0 5,573,338 - Pf6N2E2_4819 0.73 -0.8 5,573,338 - Pf6N2E2_4819 0.73 +0.4 5,573,338 - Pf6N2E2_4819 0.73 -0.2 5,573,338 - Pf6N2E2_4819 0.73 -0.0 5,573,338 - Pf6N2E2_4819 0.73 -0.5 5,573,350 + Pf6N2E2_4819 0.74 -0.5 5,573,350 + Pf6N2E2_4819 0.74 -0.3 5,573,351 - Pf6N2E2_4819 0.74 -0.6 5,573,351 - Pf6N2E2_4819 0.74 -0.1 5,573,475 + Pf6N2E2_4819 0.85 +0.4 5,573,475 + Pf6N2E2_4819 0.85 -0.5 5,573,476 - Pf6N2E2_4819 0.85 +0.5 5,573,476 - Pf6N2E2_4819 0.85 +0.1 5,573,476 - Pf6N2E2_4819 0.85 -0.1 5,573,476 - Pf6N2E2_4819 0.85 +0.9 5,573,476 - Pf6N2E2_4819 0.85 -0.0 5,573,499 - Pf6N2E2_4819 0.87 +1.0 5,573,570 + +1.6 5,573,630 - +0.1 5,573,641 + -0.2 5,573,642 - +0.1 5,573,681 + +0.4 5,573,681 + +1.3 5,573,683 + -0.0 5,573,683 + +1.3 5,573,683 + +1.4 5,573,683 + +0.4 5,573,683 + +0.4 5,573,683 + +0.3 5,573,683 + -0.8 5,573,683 + -1.5 5,573,683 + -0.3 5,573,684 - +0.7 5,573,684 - +1.0 5,573,684 - -0.3 5,573,684 - +0.8 5,573,684 - -1.0 5,574,550 + Pf6N2E2_4821 0.19 +1.8
Or see this region's nucleotide sequence