Experiment: L-Leucine (C) 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt Pf6N2E2_4657 and Pf6N2E2_4658 are separated by 31 nucleotides Pf6N2E2_4658 and Pf6N2E2_4659 are separated by 56 nucleotides Pf6N2E2_4659 and Pf6N2E2_4660 are separated by 162 nucleotides Pf6N2E2_4660 and Pf6N2E2_4661 are separated by 409 nucleotides Pf6N2E2_4661 and Pf6N2E2_4662 are separated by 186 nucleotides  
        
        Pf6N2E2_4657 - Phosphoglycerate kinase (EC 2.7.2.3), at 5,387,650 to 5,388,813 
         
        _4657 
         
        
        Pf6N2E2_4658 - FIG00955006: hypothetical protein, at 5,388,845 to 5,389,069 
         
        _4658 
         
        
        Pf6N2E2_4659 - Lipoprotein, putative, at 5,389,126 to 5,389,458 
         
        _4659 
         
        
        Pf6N2E2_4660 - Fructose-bisphosphate aldolase class II (EC 4.1.2.13), at 5,389,621 to 5,390,685 
         
        _4660 
         
        
        Pf6N2E2_4661 - KluA regulatory protein, at 5,391,095 to 5,391,373 
         
        _4661 
         
        
        Pf6N2E2_4662 - hypothetical protein, at 5,391,560 to 5,391,880 
         
        _4662 
         Position (kb)  
5389 
 
5390 
 
5391 Strain fitness (log2 ratio)  
-3 
 
-2 
 
-1 
 
0 
 
1 
 
2 
 
3 at 5388.957 kb on - strand, within Pf6N2E2_4658 at 5389.028 kb on + strand, within Pf6N2E2_4658 at 5389.028 kb on + strand, within Pf6N2E2_4658 at 5389.028 kb on + strand, within Pf6N2E2_4658 at 5389.028 kb on + strand, within Pf6N2E2_4658 at 5389.028 kb on + strand, within Pf6N2E2_4658 at 5389.028 kb on + strand, within Pf6N2E2_4658 at 5389.028 kb on + strand, within Pf6N2E2_4658 at 5389.029 kb on - strand, within Pf6N2E2_4658 at 5389.029 kb on - strand, within Pf6N2E2_4658 at 5389.083 kb on + strand at 5389.083 kb on + strand at 5389.084 kb on - strand at 5389.084 kb on - strand at 5389.084 kb on - strand at 5389.084 kb on - strand at 5389.157 kb on + strand at 5389.157 kb on + strand at 5389.157 kb on + strand at 5389.190 kb on + strand, within Pf6N2E2_4659 at 5389.191 kb on - strand, within Pf6N2E2_4659 at 5389.357 kb on + strand, within Pf6N2E2_4659 at 5389.408 kb on + strand, within Pf6N2E2_4659 at 5389.408 kb on + strand, within Pf6N2E2_4659 at 5389.408 kb on + strand, within Pf6N2E2_4659 at 5389.408 kb on + strand, within Pf6N2E2_4659 at 5389.408 kb on + strand, within Pf6N2E2_4659 at 5389.408 kb on + strand, within Pf6N2E2_4659 at 5389.408 kb on + strand, within Pf6N2E2_4659 at 5389.490 kb on + strand at 5389.492 kb on + strand at 5390.683 kb on + strand at 5390.683 kb on + strand at 5390.683 kb on + strand at 5390.684 kb on - strand at 5390.746 kb on + strand at 5390.746 kb on + strand at 5390.746 kb on + strand at 5390.747 kb on - strand at 5390.747 kb on - strand at 5390.747 kb on - strand at 5390.747 kb on - strand at 5390.747 kb on - strand at 5390.747 kb on - strand at 5390.764 kb on - strand at 5390.764 kb on - strand at 5390.772 kb on + strand at 5390.783 kb on - strand at 5390.896 kb on + strand at 5390.897 kb on - strand at 5390.897 kb on - strand at 5390.897 kb on - strand at 5390.907 kb on - strand at 5390.922 kb on + strand at 5390.922 kb on + strand at 5390.923 kb on - strand at 5390.923 kb on - strand at 5390.923 kb on - strand at 5390.923 kb on - strand at 5390.923 kb on - strand at 5390.961 kb on + strand at 5390.961 kb on + strand at 5390.961 kb on + strand at 5390.962 kb on - strand at 5390.974 kb on + strand at 5390.974 kb on + strand at 5390.974 kb on + strand at 5390.975 kb on - strand at 5390.975 kb on - strand at 5390.975 kb on - strand at 5390.975 kb on - strand at 5390.993 kb on + strand at 5390.993 kb on + strand at 5390.994 kb on - strand at 5390.994 kb on - strand at 5390.994 kb on - strand at 5391.034 kb on + strand at 5391.034 kb on + strand at 5391.034 kb on + strand at 5391.034 kb on + strand at 5391.034 kb on + strand at 5391.034 kb on + strand at 5391.034 kb on + strand at 5391.034 kb on + strand at 5391.034 kb on + strand at 5391.034 kb on + strand at 5391.034 kb on + strand at 5391.034 kb on + strand at 5391.034 kb on + strand at 5391.034 kb on + strand at 5391.034 kb on + strand at 5391.035 kb on - strand at 5391.035 kb on - strand at 5391.035 kb on - strand at 5391.035 kb on - strand at 5391.035 kb on - strand at 5391.035 kb on - strand at 5391.035 kb on - strand at 5391.035 kb on - strand at 5391.035 kb on - strand at 5391.035 kb on - strand at 5391.035 kb on - strand at 5391.035 kb on - strand at 5391.035 kb on - strand at 5391.035 kb on - strand at 5391.035 kb on - strand at 5391.038 kb on + strand at 5391.038 kb on + strand at 5391.038 kb on + strand at 5391.038 kb on + strand at 5391.038 kb on + strand at 5391.038 kb on + strand at 5391.038 kb on + strand at 5391.038 kb on + strand at 5391.038 kb on + strand at 5391.038 kb on + strand at 5391.038 kb on + strand at 5391.038 kb on + strand at 5391.038 kb on + strand at 5391.038 kb on + strand at 5391.038 kb on + strand at 5391.039 kb on - strand at 5391.039 kb on - strand at 5391.039 kb on - strand at 5391.039 kb on - strand at 5391.039 kb on - strand at 5391.039 kb on - strand at 5391.039 kb on - strand at 5391.039 kb on - strand at 5391.039 kb on - strand at 5391.039 kb on - strand at 5391.039 kb on - strand at 5391.039 kb on - strand at 5391.039 kb on - strand at 5391.039 kb on - strand at 5391.039 kb on - strand at 5391.039 kb on - strand at 5391.039 kb on - strand at 5391.039 kb on - strand at 5391.039 kb on - strand at 5391.039 kb on - strand at 5391.039 kb on - strand at 5391.039 kb on - strand at 5391.039 kb on - strand at 5391.039 kb on - strand at 5391.039 kb on - strand at 5391.039 kb on - strand at 5391.040 kb on + strand at 5391.040 kb on + strand at 5391.041 kb on - strand at 5391.041 kb on - strand at 5391.041 kb on - strand at 5391.041 kb on - strand at 5391.041 kb on - strand at 5391.041 kb on - strand at 5391.042 kb on + strand at 5391.042 kb on + strand at 5391.042 kb on + strand at 5391.042 kb on + strand at 5391.042 kb on + strand at 5391.043 kb on - strand at 5391.051 kb on + strand at 5391.051 kb on + strand at 5391.051 kb on + strand at 5391.052 kb on - strand at 5391.052 kb on - strand at 5391.088 kb on + strand at 5391.089 kb on - strand at 5391.089 kb on - strand at 5391.089 kb on - strand at 5391.089 kb on - strand at 5391.096 kb on + strand at 5391.097 kb on - strand at 5391.097 kb on - strand at 5391.192 kb on + strand, within Pf6N2E2_4661 at 5391.203 kb on + strand, within Pf6N2E2_4661 at 5391.212 kb on + strand, within Pf6N2E2_4661 at 5391.291 kb on + strand, within Pf6N2E2_4661 at 5391.291 kb on + strand, within Pf6N2E2_4661 at 5391.291 kb on + strand, within Pf6N2E2_4661 at 5391.292 kb on - strand, within Pf6N2E2_4661 at 5391.292 kb on - strand, within Pf6N2E2_4661 at 5391.292 kb on - strand, within Pf6N2E2_4661 at 5391.367 kb on + strand at 5391.367 kb on + strand at 5391.374 kb on - strand at 5391.394 kb on + strand at 5391.395 kb on - strand at 5391.395 kb on - strand at 5391.401 kb on + strand at 5391.424 kb on + strand at 5391.424 kb on + strand at 5391.424 kb on + strand at 5391.424 kb on + strand at 5391.424 kb on + strand at 5391.424 kb on + strand at 5391.424 kb on + strand at 5391.425 kb on - strand at 5391.425 kb on - strand at 5391.425 kb on - strand at 5391.459 kb on - strand at 5391.469 kb on + strand at 5391.562 kb on - strand  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   L-Leucine (C)             remove   5,388,957   -    Pf6N2E2_4658   0.50  +0.5   5,389,028   +    Pf6N2E2_4658   0.81  +0.7   5,389,028   +    Pf6N2E2_4658   0.81  +1.2   5,389,028   +    Pf6N2E2_4658   0.81  +0.5   5,389,028   +    Pf6N2E2_4658   0.81  +1.2   5,389,028   +    Pf6N2E2_4658   0.81  -0.7   5,389,028   +    Pf6N2E2_4658   0.81  -0.1   5,389,028   +    Pf6N2E2_4658   0.81  -0.2   5,389,029   -    Pf6N2E2_4658   0.82  +1.4   5,389,029   -    Pf6N2E2_4658   0.82  -0.5   5,389,083   +        +0.0   5,389,083   +        +0.4   5,389,084   -        -0.6   5,389,084   -        +0.1   5,389,084   -        -0.3   5,389,084   -        +0.1   5,389,157   +        +0.7   5,389,157   +        +0.1   5,389,157   +        -0.7   5,389,190   +    Pf6N2E2_4659   0.19  +0.5   5,389,191   -    Pf6N2E2_4659   0.20  -0.2   5,389,357   +    Pf6N2E2_4659   0.69  -0.8   5,389,408   +    Pf6N2E2_4659   0.85  -0.6   5,389,408   +    Pf6N2E2_4659   0.85  -1.6   5,389,408   +    Pf6N2E2_4659   0.85  +1.2   5,389,408   +    Pf6N2E2_4659   0.85  +0.5   5,389,408   +    Pf6N2E2_4659   0.85  +0.3   5,389,408   +    Pf6N2E2_4659   0.85  -0.1   5,389,408   +    Pf6N2E2_4659   0.85  -1.3   5,389,490   +        +0.6   5,389,492   +        +1.1   5,390,683   +        -2.0   5,390,683   +        -1.8   5,390,683   +        -0.6   5,390,684   -        -1.1   5,390,746   +        +0.7   5,390,746   +        +1.1   5,390,746   +        +0.3   5,390,747   -        -0.2   5,390,747   -        +0.1   5,390,747   -        +1.7   5,390,747   -        -2.8   5,390,747   -        +0.1   5,390,747   -        -0.5   5,390,764   -        -0.0   5,390,764   -        +0.0   5,390,772   +        -0.4   5,390,783   -        -0.7   5,390,896   +        -1.7   5,390,897   -        -1.1   5,390,897   -        +0.2   5,390,897   -        -1.3   5,390,907   -        -0.7   5,390,922   +        -0.8   5,390,922   +        +0.1   5,390,923   -        +0.6   5,390,923   -        -1.5   5,390,923   -        +1.5   5,390,923   -        -0.6   5,390,923   -        +1.2   5,390,961   +        +0.7   5,390,961   +        -0.1   5,390,961   +        +0.0   5,390,962   -        +0.1   5,390,974   +        +0.4   5,390,974   +        +1.1   5,390,974   +        -0.6   5,390,975   -        +0.2   5,390,975   -        -2.1   5,390,975   -        +0.4   5,390,975   -        -1.9   5,390,993   +        +0.6   5,390,993   +        +1.0   5,390,994   -        +0.2   5,390,994   -        -0.0   5,390,994   -        -0.6   5,391,034   +        +0.8   5,391,034   +        +0.2   5,391,034   +        +0.2   5,391,034   +        +1.0   5,391,034   +        +1.5   5,391,034   +        -0.2   5,391,034   +        +3.0   5,391,034   +        -0.6   5,391,034   +        +1.5   5,391,034   +        +0.7   5,391,034   +        +0.7   5,391,034   +        +0.2   5,391,034   +        +0.1   5,391,034   +        -0.4   5,391,034   +        -0.2   5,391,035   -        +0.1   5,391,035   -        -0.3   5,391,035   -        -0.0   5,391,035   -        +0.2   5,391,035   -        +0.0   5,391,035   -        +0.7   5,391,035   -        -0.4   5,391,035   -        +0.3   5,391,035   -        +0.1   5,391,035   -        +0.3   5,391,035   -        -1.2   5,391,035   -        +0.3   5,391,035   -        +0.1   5,391,035   -        +0.6   5,391,035   -        -1.2   5,391,038   +        +0.1   5,391,038   +        -1.1   5,391,038   +        -0.4   5,391,038   +        -0.4   5,391,038   +        -0.5   5,391,038   +        +0.0   5,391,038   +        -0.8   5,391,038   +        -0.5   5,391,038   +        -0.0   5,391,038   +        +0.7   5,391,038   +        +1.5   5,391,038   +        +1.0   5,391,038   +        +0.9   5,391,038   +        -0.6   5,391,038   +        +0.6   5,391,039   -        -0.3   5,391,039   -        -0.4   5,391,039   -        -0.0   5,391,039   -        +0.7   5,391,039   -        +0.2   5,391,039   -        +0.4   5,391,039   -        -1.1   5,391,039   -        +0.6   5,391,039   -        -0.9   5,391,039   -        -0.8   5,391,039   -        -0.4   5,391,039   -        -0.0   5,391,039   -        -0.5   5,391,039   -        -0.3   5,391,039   -        +0.7   5,391,039   -        +0.5   5,391,039   -        +1.4   5,391,039   -        +0.7   5,391,039   -        +0.6   5,391,039   -        +0.1   5,391,039   -        -0.3   5,391,039   -        +0.9   5,391,039   -        +0.1   5,391,039   -        -2.1   5,391,039   -        +0.1   5,391,039   -        +0.8   5,391,040   +        +1.5   5,391,040   +        +0.9   5,391,041   -        +0.0   5,391,041   -        +0.7   5,391,041   -        -0.6   5,391,041   -        -0.1   5,391,041   -        +0.5   5,391,041   -        -0.5   5,391,042   +        +0.1   5,391,042   +        -0.7   5,391,042   +        +1.4   5,391,042   +        -0.1   5,391,042   +        +0.5   5,391,043   -        +0.4   5,391,051   +        -0.1   5,391,051   +        +0.9   5,391,051   +        -1.7   5,391,052   -        -0.6   5,391,052   -        -1.0   5,391,088   +        +1.0   5,391,089   -        +0.1   5,391,089   -        -0.8   5,391,089   -        -0.2   5,391,089   -        -0.7   5,391,096   +        +1.9   5,391,097   -        +0.6   5,391,097   -        -0.6   5,391,192   +    Pf6N2E2_4661   0.35  +0.6   5,391,203   +    Pf6N2E2_4661   0.39  -0.1   5,391,212   +    Pf6N2E2_4661   0.42  +0.9   5,391,291   +    Pf6N2E2_4661   0.70  -0.1   5,391,291   +    Pf6N2E2_4661   0.70  -1.6   5,391,291   +    Pf6N2E2_4661   0.70  -0.4   5,391,292   -    Pf6N2E2_4661   0.71  +0.9   5,391,292   -    Pf6N2E2_4661   0.71  -0.6   5,391,292   -    Pf6N2E2_4661   0.71  +0.1   5,391,367   +        +0.5   5,391,367   +        -0.3   5,391,374   -        -1.0   5,391,394   +        +1.2   5,391,395   -        -0.4   5,391,395   -        +0.2   5,391,401   +        +0.0   5,391,424   +        +1.9   5,391,424   +        -0.3   5,391,424   +        -0.9   5,391,424   +        -0.1   5,391,424   +        +0.2   5,391,424   +        +0.3   5,391,424   +        -0.5   5,391,425   -        +0.2   5,391,425   -        +0.9   5,391,425   -        +0.9   5,391,459   -        -0.9   5,391,469   +        -0.4   5,391,562   -        -0.2 
 
Or see this region's nucleotide sequence