Experiment: MRS + carbenicillin Time48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS20005 and BBR_RS20010 are separated by 325 nucleotides BBR_RS20010 and BBR_RS20015 are separated by 191 nucleotides
BBR_RS20005: BBR_RS20005 - Sugar-binding protein of ABC transporter system, at 2,286,888 to 2,288,066
_RS20005
BBR_RS20010: BBR_RS20010 - transposase, at 2,288,392 to 2,288,805
_RS20010
BBR_RS20015: BBR_RS20015 - IS21 family transposase, at 2,288,997 to 2,290,478
_RS20015
Position (kb)
2288
2289 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2287.407 kb on - strand, within BBR_RS20005 at 2287.417 kb on + strand, within BBR_RS20005 at 2287.452 kb on - strand, within BBR_RS20005 at 2287.454 kb on + strand, within BBR_RS20005 at 2287.500 kb on + strand, within BBR_RS20005 at 2287.501 kb on - strand, within BBR_RS20005 at 2287.502 kb on + strand, within BBR_RS20005 at 2287.502 kb on + strand, within BBR_RS20005 at 2287.508 kb on - strand, within BBR_RS20005 at 2287.508 kb on - strand, within BBR_RS20005 at 2287.509 kb on - strand, within BBR_RS20005 at 2287.509 kb on - strand, within BBR_RS20005 at 2287.538 kb on + strand, within BBR_RS20005 at 2287.542 kb on - strand, within BBR_RS20005 at 2287.547 kb on + strand, within BBR_RS20005 at 2287.548 kb on + strand, within BBR_RS20005 at 2287.548 kb on + strand, within BBR_RS20005 at 2287.554 kb on + strand, within BBR_RS20005 at 2287.562 kb on - strand, within BBR_RS20005 at 2287.562 kb on - strand, within BBR_RS20005 at 2287.613 kb on + strand, within BBR_RS20005 at 2287.685 kb on + strand, within BBR_RS20005 at 2287.707 kb on + strand, within BBR_RS20005 at 2287.714 kb on + strand, within BBR_RS20005 at 2287.722 kb on - strand, within BBR_RS20005 at 2287.759 kb on + strand, within BBR_RS20005 at 2287.761 kb on + strand, within BBR_RS20005 at 2287.775 kb on - strand, within BBR_RS20005 at 2287.775 kb on - strand, within BBR_RS20005 at 2287.775 kb on - strand, within BBR_RS20005 at 2287.782 kb on + strand, within BBR_RS20005 at 2287.785 kb on - strand, within BBR_RS20005 at 2287.785 kb on - strand, within BBR_RS20005 at 2287.790 kb on - strand, within BBR_RS20005 at 2287.795 kb on - strand, within BBR_RS20005 at 2287.798 kb on + strand, within BBR_RS20005 at 2287.798 kb on + strand, within BBR_RS20005 at 2287.806 kb on - strand, within BBR_RS20005 at 2287.806 kb on - strand, within BBR_RS20005 at 2287.806 kb on - strand, within BBR_RS20005 at 2287.806 kb on - strand, within BBR_RS20005 at 2287.808 kb on - strand, within BBR_RS20005 at 2287.808 kb on - strand, within BBR_RS20005 at 2287.808 kb on - strand, within BBR_RS20005 at 2287.808 kb on - strand, within BBR_RS20005 at 2287.808 kb on - strand, within BBR_RS20005 at 2287.808 kb on - strand, within BBR_RS20005 at 2287.813 kb on + strand, within BBR_RS20005 at 2287.813 kb on + strand, within BBR_RS20005 at 2287.821 kb on + strand, within BBR_RS20005 at 2287.829 kb on - strand, within BBR_RS20005 at 2287.839 kb on + strand, within BBR_RS20005 at 2287.847 kb on - strand, within BBR_RS20005 at 2287.853 kb on + strand, within BBR_RS20005 at 2287.885 kb on + strand, within BBR_RS20005 at 2287.887 kb on + strand, within BBR_RS20005 at 2287.890 kb on + strand, within BBR_RS20005 at 2287.890 kb on + strand, within BBR_RS20005 at 2287.891 kb on + strand, within BBR_RS20005 at 2287.898 kb on - strand, within BBR_RS20005 at 2287.903 kb on - strand, within BBR_RS20005 at 2287.922 kb on - strand, within BBR_RS20005 at 2287.922 kb on - strand, within BBR_RS20005 at 2287.925 kb on + strand, within BBR_RS20005 at 2287.933 kb on - strand, within BBR_RS20005 at 2287.935 kb on + strand, within BBR_RS20005 at 2287.997 kb on + strand at 2288.006 kb on + strand at 2288.012 kb on - strand at 2288.016 kb on + strand at 2288.019 kb on - strand at 2288.042 kb on + strand at 2288.079 kb on + strand at 2288.089 kb on + strand at 2288.089 kb on + strand at 2288.089 kb on + strand at 2288.089 kb on + strand at 2288.089 kb on + strand at 2288.089 kb on + strand at 2288.097 kb on - strand at 2288.097 kb on - strand at 2288.097 kb on - strand at 2288.123 kb on + strand at 2288.145 kb on + strand at 2288.145 kb on + strand at 2288.240 kb on + strand at 2288.277 kb on + strand at 2288.285 kb on + strand at 2288.324 kb on + strand at 2288.324 kb on + strand at 2288.347 kb on + strand at 2288.348 kb on - strand at 2288.348 kb on - strand at 2288.348 kb on - strand at 2288.348 kb on - strand at 2288.363 kb on + strand at 2288.363 kb on + strand at 2288.381 kb on - strand at 2288.381 kb on - strand at 2288.391 kb on - strand at 2288.484 kb on - strand, within BBR_RS20010 at 2288.484 kb on - strand, within BBR_RS20010 at 2288.484 kb on - strand, within BBR_RS20010 at 2288.484 kb on - strand, within BBR_RS20010 at 2288.484 kb on - strand, within BBR_RS20010 at 2288.484 kb on - strand, within BBR_RS20010 at 2288.484 kb on - strand, within BBR_RS20010 at 2288.506 kb on - strand, within BBR_RS20010 at 2288.507 kb on - strand, within BBR_RS20010 at 2288.519 kb on + strand, within BBR_RS20010 at 2288.527 kb on - strand, within BBR_RS20010 at 2288.528 kb on - strand, within BBR_RS20010 at 2288.536 kb on + strand, within BBR_RS20010 at 2288.547 kb on + strand, within BBR_RS20010 at 2288.550 kb on - strand, within BBR_RS20010 at 2288.563 kb on - strand, within BBR_RS20010 at 2288.563 kb on - strand, within BBR_RS20010 at 2288.564 kb on - strand, within BBR_RS20010 at 2288.587 kb on - strand, within BBR_RS20010 at 2288.587 kb on - strand, within BBR_RS20010 at 2288.626 kb on - strand, within BBR_RS20010 at 2288.639 kb on + strand, within BBR_RS20010 at 2288.640 kb on + strand, within BBR_RS20010 at 2288.657 kb on + strand, within BBR_RS20010 at 2288.657 kb on + strand, within BBR_RS20010 at 2288.657 kb on + strand, within BBR_RS20010 at 2288.657 kb on + strand, within BBR_RS20010 at 2288.665 kb on - strand, within BBR_RS20010 at 2288.692 kb on + strand, within BBR_RS20010 at 2288.702 kb on + strand, within BBR_RS20010 at 2288.720 kb on + strand, within BBR_RS20010 at 2288.720 kb on + strand, within BBR_RS20010 at 2288.720 kb on - strand, within BBR_RS20010 at 2288.728 kb on - strand, within BBR_RS20010 at 2288.732 kb on + strand, within BBR_RS20010 at 2288.734 kb on + strand, within BBR_RS20010 at 2288.744 kb on + strand, within BBR_RS20010 at 2288.749 kb on + strand, within BBR_RS20010 at 2288.755 kb on + strand, within BBR_RS20010 at 2288.757 kb on - strand, within BBR_RS20010 at 2288.757 kb on - strand, within BBR_RS20010 at 2288.757 kb on - strand, within BBR_RS20010 at 2288.757 kb on - strand, within BBR_RS20010 at 2288.778 kb on - strand at 2288.778 kb on - strand at 2288.778 kb on - strand at 2288.778 kb on - strand at 2288.795 kb on + strand at 2288.795 kb on + strand at 2288.795 kb on + strand at 2288.795 kb on + strand at 2288.795 kb on + strand at 2288.797 kb on + strand at 2288.797 kb on + strand at 2288.800 kb on + strand at 2288.800 kb on + strand at 2288.800 kb on + strand at 2288.803 kb on - strand at 2288.803 kb on - strand at 2288.803 kb on - strand at 2288.803 kb on - strand at 2288.803 kb on - strand at 2288.808 kb on - strand at 2288.808 kb on - strand at 2288.847 kb on - strand at 2288.847 kb on - strand at 2288.861 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MRS + carbenicillin Time48hr remove 2,287,407 - BBR_RS20005 0.44 -1.6 2,287,417 + BBR_RS20005 0.45 +1.0 2,287,452 - BBR_RS20005 0.48 +2.2 2,287,454 + BBR_RS20005 0.48 -1.2 2,287,500 + BBR_RS20005 0.52 -0.6 2,287,501 - BBR_RS20005 0.52 -0.0 2,287,502 + BBR_RS20005 0.52 -2.3 2,287,502 + BBR_RS20005 0.52 -2.1 2,287,508 - BBR_RS20005 0.53 +0.9 2,287,508 - BBR_RS20005 0.53 +1.9 2,287,509 - BBR_RS20005 0.53 -2.1 2,287,509 - BBR_RS20005 0.53 +0.7 2,287,538 + BBR_RS20005 0.55 +0.3 2,287,542 - BBR_RS20005 0.55 -0.7 2,287,547 + BBR_RS20005 0.56 -1.1 2,287,548 + BBR_RS20005 0.56 +4.2 2,287,548 + BBR_RS20005 0.56 -2.3 2,287,554 + BBR_RS20005 0.56 -3.1 2,287,562 - BBR_RS20005 0.57 +1.9 2,287,562 - BBR_RS20005 0.57 +1.7 2,287,613 + BBR_RS20005 0.61 -0.3 2,287,685 + BBR_RS20005 0.68 +3.2 2,287,707 + BBR_RS20005 0.69 -0.5 2,287,714 + BBR_RS20005 0.70 -2.7 2,287,722 - BBR_RS20005 0.71 +0.9 2,287,759 + BBR_RS20005 0.74 -2.8 2,287,761 + BBR_RS20005 0.74 +1.4 2,287,775 - BBR_RS20005 0.75 +0.1 2,287,775 - BBR_RS20005 0.75 -0.1 2,287,775 - BBR_RS20005 0.75 -0.6 2,287,782 + BBR_RS20005 0.76 +0.1 2,287,785 - BBR_RS20005 0.76 -0.9 2,287,785 - BBR_RS20005 0.76 -1.9 2,287,790 - BBR_RS20005 0.77 +0.9 2,287,795 - BBR_RS20005 0.77 -1.4 2,287,798 + BBR_RS20005 0.77 +0.3 2,287,798 + BBR_RS20005 0.77 -1.2 2,287,806 - BBR_RS20005 0.78 +0.9 2,287,806 - BBR_RS20005 0.78 -3.1 2,287,806 - BBR_RS20005 0.78 +0.4 2,287,806 - BBR_RS20005 0.78 -1.7 2,287,808 - BBR_RS20005 0.78 -1.7 2,287,808 - BBR_RS20005 0.78 +0.9 2,287,808 - BBR_RS20005 0.78 -0.1 2,287,808 - BBR_RS20005 0.78 +2.5 2,287,808 - BBR_RS20005 0.78 -0.0 2,287,808 - BBR_RS20005 0.78 -1.4 2,287,813 + BBR_RS20005 0.78 +2.1 2,287,813 + BBR_RS20005 0.78 -0.1 2,287,821 + BBR_RS20005 0.79 -1.9 2,287,829 - BBR_RS20005 0.80 -0.3 2,287,839 + BBR_RS20005 0.81 +0.3 2,287,847 - BBR_RS20005 0.81 -2.4 2,287,853 + BBR_RS20005 0.82 -0.2 2,287,885 + BBR_RS20005 0.85 -1.4 2,287,887 + BBR_RS20005 0.85 +0.9 2,287,890 + BBR_RS20005 0.85 -2.6 2,287,890 + BBR_RS20005 0.85 +0.3 2,287,891 + BBR_RS20005 0.85 +0.9 2,287,898 - BBR_RS20005 0.86 -0.7 2,287,903 - BBR_RS20005 0.86 +0.4 2,287,922 - BBR_RS20005 0.88 +0.7 2,287,922 - BBR_RS20005 0.88 +0.9 2,287,925 + BBR_RS20005 0.88 +1.2 2,287,933 - BBR_RS20005 0.89 -0.1 2,287,935 + BBR_RS20005 0.89 +0.9 2,287,997 + +0.3 2,288,006 + -1.9 2,288,012 - +1.9 2,288,016 + -2.0 2,288,019 - -0.3 2,288,042 + -4.2 2,288,079 + -1.0 2,288,089 + -1.1 2,288,089 + +0.1 2,288,089 + -0.6 2,288,089 + -0.1 2,288,089 + -0.1 2,288,089 + -1.1 2,288,097 - +0.9 2,288,097 - +1.9 2,288,097 - -1.4 2,288,123 + +0.3 2,288,145 + +0.9 2,288,145 + -0.0 2,288,240 + +0.2 2,288,277 + +0.9 2,288,285 + -0.4 2,288,324 + +0.1 2,288,324 + +0.9 2,288,347 + -0.7 2,288,348 - -1.4 2,288,348 - -0.5 2,288,348 - -0.1 2,288,348 - +1.4 2,288,363 + -4.1 2,288,363 + +0.9 2,288,381 - +1.6 2,288,381 - +2.9 2,288,391 - -3.4 2,288,484 - BBR_RS20010 0.22 -0.1 2,288,484 - BBR_RS20010 0.22 -1.4 2,288,484 - BBR_RS20010 0.22 -0.5 2,288,484 - BBR_RS20010 0.22 -0.1 2,288,484 - BBR_RS20010 0.22 -0.7 2,288,484 - BBR_RS20010 0.22 +0.9 2,288,484 - BBR_RS20010 0.22 -2.1 2,288,506 - BBR_RS20010 0.28 -2.3 2,288,507 - BBR_RS20010 0.28 -0.7 2,288,519 + BBR_RS20010 0.31 +0.7 2,288,527 - BBR_RS20010 0.33 +2.9 2,288,528 - BBR_RS20010 0.33 -2.2 2,288,536 + BBR_RS20010 0.35 -3.8 2,288,547 + BBR_RS20010 0.37 -2.0 2,288,550 - BBR_RS20010 0.38 -2.7 2,288,563 - BBR_RS20010 0.41 -0.7 2,288,563 - BBR_RS20010 0.41 -2.4 2,288,564 - BBR_RS20010 0.42 -0.1 2,288,587 - BBR_RS20010 0.47 +1.9 2,288,587 - BBR_RS20010 0.47 -1.1 2,288,626 - BBR_RS20010 0.57 +0.9 2,288,639 + BBR_RS20010 0.60 +1.7 2,288,640 + BBR_RS20010 0.60 +0.3 2,288,657 + BBR_RS20010 0.64 +2.9 2,288,657 + BBR_RS20010 0.64 -0.6 2,288,657 + BBR_RS20010 0.64 +0.5 2,288,657 + BBR_RS20010 0.64 -0.9 2,288,665 - BBR_RS20010 0.66 +0.9 2,288,692 + BBR_RS20010 0.72 -2.3 2,288,702 + BBR_RS20010 0.75 +0.9 2,288,720 + BBR_RS20010 0.79 -0.1 2,288,720 + BBR_RS20010 0.79 +0.9 2,288,720 - BBR_RS20010 0.79 -1.3 2,288,728 - BBR_RS20010 0.81 -2.0 2,288,732 + BBR_RS20010 0.82 -0.5 2,288,734 + BBR_RS20010 0.83 -1.1 2,288,744 + BBR_RS20010 0.85 +3.7 2,288,749 + BBR_RS20010 0.86 -1.7 2,288,755 + BBR_RS20010 0.88 -2.3 2,288,757 - BBR_RS20010 0.88 -3.0 2,288,757 - BBR_RS20010 0.88 +1.9 2,288,757 - BBR_RS20010 0.88 +0.9 2,288,757 - BBR_RS20010 0.88 +1.2 2,288,778 - +0.2 2,288,778 - +1.9 2,288,778 - +3.5 2,288,778 - -0.4 2,288,795 + -0.1 2,288,795 + +0.1 2,288,795 + +0.7 2,288,795 + -1.3 2,288,795 + +1.6 2,288,797 + +1.9 2,288,797 + +0.9 2,288,800 + -2.3 2,288,800 + +1.8 2,288,800 + -0.1 2,288,803 - -1.1 2,288,803 - -2.4 2,288,803 - -1.7 2,288,803 - -0.3 2,288,803 - -1.0 2,288,808 - -0.9 2,288,808 - +0.9 2,288,847 - +0.3 2,288,847 - -1.1 2,288,861 - -1.1
Or see this region's nucleotide sequence