Experiment: MRS + carbenicillin Time48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16160 and BBR_RS16165 are separated by 12 nucleotides BBR_RS16165 and BBR_RS16170 are separated by 214 nucleotides BBR_RS16170 and BBR_RS16175 are separated by 105 nucleotides BBR_RS16175 and BBR_RS16180 are separated by 174 nucleotides
BBR_RS16160: BBR_RS16160 - hypothetical protein, at 1,374,071 to 1,375,756
_RS16160
BBR_RS16165: BBR_RS16165 - M23 family peptidase, at 1,375,769 to 1,376,392
_RS16165
BBR_RS16170: BBR_RS16170 - hypothetical protein, at 1,376,607 to 1,376,831
_RS16170
BBR_RS16175: BBR_RS16175 - DNA integration/recombination/inversion protein, at 1,376,937 to 1,377,320
_RS16175
BBR_RS16180: BBR_RS16180 - IS21 family transposase, at 1,377,495 to 1,378,988
_RS16180
Position (kb)
1376
1377 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1375.867 kb on - strand, within BBR_RS16165 at 1375.869 kb on + strand, within BBR_RS16165 at 1375.869 kb on + strand, within BBR_RS16165 at 1375.869 kb on + strand, within BBR_RS16165 at 1375.884 kb on - strand, within BBR_RS16165 at 1375.907 kb on - strand, within BBR_RS16165 at 1375.917 kb on - strand, within BBR_RS16165 at 1375.975 kb on - strand, within BBR_RS16165 at 1375.980 kb on - strand, within BBR_RS16165 at 1376.002 kb on - strand, within BBR_RS16165 at 1376.061 kb on + strand, within BBR_RS16165 at 1376.069 kb on - strand, within BBR_RS16165 at 1376.137 kb on - strand, within BBR_RS16165 at 1376.166 kb on - strand, within BBR_RS16165 at 1376.289 kb on - strand, within BBR_RS16165 at 1376.331 kb on - strand at 1376.396 kb on - strand at 1376.410 kb on + strand at 1376.413 kb on - strand at 1376.486 kb on + strand at 1376.769 kb on + strand, within BBR_RS16170 at 1376.771 kb on + strand, within BBR_RS16170 at 1376.831 kb on + strand at 1376.839 kb on - strand at 1376.889 kb on + strand at 1376.925 kb on + strand at 1376.930 kb on - strand at 1376.945 kb on + strand at 1376.972 kb on - strand at 1377.054 kb on - strand, within BBR_RS16175 at 1377.061 kb on - strand, within BBR_RS16175 at 1377.061 kb on - strand, within BBR_RS16175 at 1377.085 kb on - strand, within BBR_RS16175 at 1377.174 kb on - strand, within BBR_RS16175 at 1377.181 kb on - strand, within BBR_RS16175 at 1377.207 kb on - strand, within BBR_RS16175 at 1377.248 kb on + strand, within BBR_RS16175 at 1377.287 kb on + strand at 1377.287 kb on + strand at 1377.287 kb on + strand at 1377.287 kb on + strand at 1377.287 kb on + strand at 1377.293 kb on - strand at 1377.295 kb on - strand at 1377.295 kb on - strand at 1377.370 kb on + strand at 1377.378 kb on + strand at 1377.386 kb on - strand at 1377.393 kb on + strand at 1377.401 kb on - strand at 1377.401 kb on - strand at 1377.411 kb on - strand at 1377.420 kb on + strand at 1377.522 kb on + strand at 1377.541 kb on + strand at 1377.551 kb on + strand at 1377.551 kb on + strand at 1377.557 kb on - strand at 1377.564 kb on - strand at 1377.587 kb on - strand at 1377.628 kb on + strand at 1377.664 kb on + strand, within BBR_RS16180 at 1377.706 kb on - strand, within BBR_RS16180 at 1377.719 kb on + strand, within BBR_RS16180 at 1377.809 kb on - strand, within BBR_RS16180 at 1377.823 kb on + strand, within BBR_RS16180
Per-strain Table
Position Strand Gene LocusTag Fraction MRS + carbenicillin Time48hr remove 1,375,867 - BBR_RS16165 0.16 -1.3 1,375,869 + BBR_RS16165 0.16 -2.1 1,375,869 + BBR_RS16165 0.16 +1.2 1,375,869 + BBR_RS16165 0.16 +1.0 1,375,884 - BBR_RS16165 0.18 +0.7 1,375,907 - BBR_RS16165 0.22 +1.6 1,375,917 - BBR_RS16165 0.24 -1.8 1,375,975 - BBR_RS16165 0.33 -1.8 1,375,980 - BBR_RS16165 0.34 +0.4 1,376,002 - BBR_RS16165 0.37 +1.6 1,376,061 + BBR_RS16165 0.47 +1.0 1,376,069 - BBR_RS16165 0.48 +1.6 1,376,137 - BBR_RS16165 0.59 -1.3 1,376,166 - BBR_RS16165 0.64 -1.2 1,376,289 - BBR_RS16165 0.83 -1.6 1,376,331 - +1.0 1,376,396 - +0.0 1,376,410 + -0.4 1,376,413 - +1.7 1,376,486 + -2.8 1,376,769 + BBR_RS16170 0.72 -0.6 1,376,771 + BBR_RS16170 0.73 -2.3 1,376,831 + +1.4 1,376,839 - -1.3 1,376,889 + -1.0 1,376,925 + +1.0 1,376,930 - +0.0 1,376,945 + +1.0 1,376,972 - +2.6 1,377,054 - BBR_RS16175 0.30 +1.0 1,377,061 - BBR_RS16175 0.32 +0.0 1,377,061 - BBR_RS16175 0.32 +1.0 1,377,085 - BBR_RS16175 0.39 +1.3 1,377,174 - BBR_RS16175 0.62 +0.0 1,377,181 - BBR_RS16175 0.64 +1.0 1,377,207 - BBR_RS16175 0.70 -1.0 1,377,248 + BBR_RS16175 0.81 +0.0 1,377,287 + -0.3 1,377,287 + -1.4 1,377,287 + -1.4 1,377,287 + -1.6 1,377,287 + -1.7 1,377,293 - -1.0 1,377,295 - +2.0 1,377,295 - +1.0 1,377,370 + -0.8 1,377,378 + -1.3 1,377,386 - +3.0 1,377,393 + -1.2 1,377,401 - -0.2 1,377,401 - +0.0 1,377,411 - +0.0 1,377,420 + -0.4 1,377,522 + -1.0 1,377,541 + -1.7 1,377,551 + -1.7 1,377,551 + +0.0 1,377,557 - +1.0 1,377,564 - +0.0 1,377,587 - +0.0 1,377,628 + +1.0 1,377,664 + BBR_RS16180 0.11 -1.2 1,377,706 - BBR_RS16180 0.14 +0.0 1,377,719 + BBR_RS16180 0.15 +2.0 1,377,809 - BBR_RS16180 0.21 +1.5 1,377,823 + BBR_RS16180 0.22 +0.8
Or see this region's nucleotide sequence